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Last Updated: March 28, 2024

Claims for Patent: 6,368,845


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Summary for Patent: 6,368,845
Title: Polypeptides having L-asparaginase activity
Abstract:Disclosed are polypeptides which originate from mammal, having L-asparaginase activity. The polypeptides are easily prepared by applying recombinant DNA techniques to DNAs encoding the polypeptides and they exert satisfactory effects in the treatment and/or the prevention for diseases caused by tumor cells dependent on L-asparagine, and cause no substantial serious side effects even when administered to humans in relatively-high dose.
Inventor(s): Ario; Takeshi (Okayama, JP), Taniai; Madoka (Okayama, JP), Yamamoto; Kozo (Okayama, JP), Kurimoto; Masashi (Okayama, JP)
Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo (JP)
Application Number:09/635,705
Patent Claims:1. An isolated DNA molecule encoding a polypeptide having L-asparaginase activity, wherein said DNA molecule consists of either:

(a) a nucleotide sequence consisting of a part of SEQ ID NO:14 with the remainder of SEQ ID NO:14 substituted with the corresponding part of SEQ ID NO:10; or

(b) a fragment of the nucleotide sequence of (a), which fragment contains a part of SEQ ID NO:14 and a part of SEQ ID NO:10, and which encodes a polypeptide having L-asparaginase activity.

2. A self-replicable vector, comprising an isolated DNA molecule encoding the polypeptide having L-asparaginase activity according to claim 1.

3. A host cell transformed by the self-replicable vector according to claim 2.

4. The host cell according to claim 3, wherein said host cell is selected from the group consisting of prokaryotic and eukaryotic cells.

5. The host cell according to claim 3, wherein said host cell is Escherichia coli.

6. The host cell according to claim 3, wherein said host cell is a mouse cell.

7. The self-replicable vector according to claim 2, which is a plasmid vector.

8. The self-replicable vector according to claim 2, further comprising one or more promoters selected from the group consisting of metallothionein and Tac promoters.

9. A host cell transformed with a DNA molecule according to claim 1.

10. The host cell according to claim 9, wherein said host cell is a member selected from the group consisting of prokaryotic and eukaryotic cells.

11. The host cell according to claim 9, wherein said host cell is Escherichia coli.

12. The host cell according to claim 9, wherein said host cell is a mouse cell.

13. A process for preparing a polypeptide having L-asparaginase activity, comprising:

expressing a DNA molecule encoding the polypeptide according to claim 1; and

collecting the expressed polypeptide.

14. The process according to claim 13, wherein the expressing step comprises culturing a host cell transformed with the DNA molecule encoding the polypeptide.

15. The process according to claim 13, wherein the collecting step comprises one or more techniques selected from the group consisting of salting out, dialysis, filtration, concentration, gel filtration chromatography, ion-exchange chromatography, affinity chromatography, hydrophobic chromatography, isoelectric focusing and gel electrophoresis.

16. An isolated DNA molecule encoding a polypeptide having L-asparaginase activity, wherein the DNA molecule consists of a fragment of the nucleotide sequence of SEQ ID NO:17, wherein said fragment is a 5'-terminal part of SEQ ID NO:17 consisting of over 1,011 nucleotides and encodes a polypeptide having L-asparaginase activity.

17. A self-replicable vector, comprising an isolated DNA molecule encoding the polypeptide having L-asparaginase activity according to claim 16.

18. A host cell transformed by the self-replicable vector according to claim 17.

19. The host cell according to claim 18, wherein said host cell is a member selected from the group consisting of prokaryotic and eukaryotic cells.

20. The host cell according to claim 18, wherein said host cell is Escherichia coli.

21. The host cell according to claim 18, wherein said host cell is a mouse cell.

22. The self-replicable vector according to claim 17, which is a plasmid vector.

23. The self-replicable vector according to claim 17, further comprising one or more promoters selected from the group consisting of metallothionein and Tac promoters.

24. A host cell transformed with a DNA molecule according to claim 16.

25. The host cell according to claim 24, wherein said host cell is a member selected from the group consisting of prokaryotic and eukaryotic cells.

26. The host cell according to claim 24, wherein said host cell is Escherichia coli.

27. The host cell according to claim 24, wherein said host cell is a mouse cell.

28. A process for preparing a polypeptide having L-asparaginase activity, comprising:

expressing a DNA molecule encoding the polypeptide according to claim 16; and

collecting the expressed polypeptide.

29. The process according to claim 28, wherein the expressing step comprises culturing a host cell transformed with the DNA molecule encoding the polypeptide.

30. The process according to the claim 28, wherein the collecting step comprises one or more techniques selected from the group consisting of salting out, dialysis, filtration, concentration, gel filtration chromatography, affinity chromatography, hydrophobic chromatography, isoelectric focusing and gel electrophoresis.

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