Claims for Patent: 6,297,014

Summary for Patent: 6,297,014

Title:	Genetic test to determine non-responsiveness to statin drug treatment
Abstract:	In a method for detecting a genetic predisposition in a human for non-responsiveness to statin drug treatment for coronary artery disease, nucleic acids comprising nucleotide sequences of the human lipoprotein lipase (LPL) gene are amplified and analyzed. Homozygosity for a variant allele in a non-coding or untranslated region of the 3\' end of LPL, for example, LPL HindIII 2/2 or (TTTA).sub.n 4/4 genotypes, is linked to non-responsiveness to treatment with statin drugs, including lovastatin, pravastatin, simvastatin, atorvastatin, fluvastatin, or cerivastatin. Oligonucleotide primer sequences, primer sets, and genetic testing kits allow the practitioner to practice the method and thus better individualize the treatment and improve the care of patients with coronary artery disease.
Inventor(s):	Taylor; Kent D. (Santa Paula, CA), Scheuner; Maren T. (Manhattan Beach, CA), Rotter; Jerome I. (Los Angeles, CA), Yang; Huiying (Cerritos, CA)
Assignee:	Cedars-Sinai Medical Center (Los Angeles, CA)
Application Number:	09/347,114
Patent Claims:	1. A method of detecting a genetic predisposition in a human subject for non-responsiveness to treatment with a statin drug selected from the group consisting of lovastatin, pravastatin, and simvastatin, comprising: a) collecting a tissue sample from a human subject; b) amplifying nucleic acids that include the normal locus of the HindIII recognition site in intron 8 of the human lipoprotein lipase gene (LPL) from said tissue sample to obtain amplification products; and c) analyzing the amplification products for the absence of a HindIII recognition site in intron 8 of the human lipoprotein lipase gene, homozygosity for an absence of said HindIII recognition site indicating a genetic predisposition for non-responsiveness to treatment with a statin drug selected from the group consisting of lovastatin, pravastatin, and simvastatin. 2. The method of claim 1, wherein the tissue sample is a blood sample. 3. The method of claim 1, further comprising restricting the amplification products with a restriction enzyme before analyzing the amplification products. 4. The method of claim 1, wherein the restriction enzyme is HindIII. 5. The method of claim 1, wherein an oligonucleotide primer is used in amplifying said nucleic acids. 6. The method of claim 1, wherein an oligonucleotide primer comprising the sequence GCA TCT GCC TTC AGC TAG ACA TTG (SEQ. ID. NO. 1) is used in amplifying said nucleic acids. 7. The method of claim 1, wherein an oligonucleotide primer comprising the sequence TCT TCC AGA AGG GTG AGA TTC CAA (SEQ. ID. NO. 2) is used in amplifying said nucleic acids. 8. The method of claim 1, wherein an oligonucleotide primer having the sequence GCA TCT GCC TTC AGC TAG ACA TTG (SEQ. ID. NO. 1) or TCT TCC AGA AGG GTG AGA TTC CAA (SEQ. ID. NO. 2) is used in amplifying said nucleic acids. 9. The method of claim 1, wherein a reverse oligonucleotide primer having the sequence 5'-GCA TCT GCC TTC AGC TAG ACA TTG-3' (SEQ. ID. NO. 1) and a forward oligonucleotide primer having the sequence 5'-TCT TCC AGA AGG GTG AGA TTC CAA-3' (SEQ. ID. NO. 2) are used in amplifying said nucleic acids. 10. The method of claim 1, wherein an oligonucleotide primer is used in amplifying said nucleic acids, said primer comprising a sequence selected from the group essentially consisting of (SEQ. ID. NO.:1), (SEQ. ID. NO.:2), (SEQ. ID. NO.:3), (SEQ. ID. NO.:4), (SEQ. ID. NO.:5), (SEQ. ID. NO.:6), (SEQ. ID. NO.:7), (SEQ. ID. NO.:8), (SEQ. ID. NO.:9), (SEQ. ID. NO.:10), (SEQ. ID. NO.:10), (SEQ. ID. NO.:11), (SEQ. ID. NO.:12), (SEQ. ID. NO.:13), (SEQ. ID. NO.:14), (SEQ. ID. NO.:15), (SEQ. ID. NO.:16), (SEQ. ID. NO.:17), (SEQ. ID. NO.:18), (SEQ. ID. NO.:19), (SEQ. ID. NO.:20), (SEQ. ID. NO.:21), (SEQ. ID. NO.:22), (SEQ. ID. NO.:23), (SEQ. ID. NO.:24), (SEQ. ID. NO.:25), (SEQ. ID. NO.:26), (SEQ. ID. NO.:27), (SEQ. ID. NO.:28), (SEQ. ID. NO.:29), (SEQ. ID. NO.:30), (SEQ. ID. NO.:31), (SEQ. ID. NO.:32), (SEQ. ID. NO.:35), (SEQ. ID. NO.:36), (SEQ. ID. NO.:37), (SEQ. ID. NO.:38), (SEQ. ID. NO.:39), (SEQ. ID. NO.:40), (SEQ. ID. NO.:41), (SEQ. ID. NO.:42), (SEQ. ID. NO.:43), (SEQ. ID. NO.:44), (SEQ. ID. NO.:45),(SEQ. ID. NO.:46), (SEQ. ID. NO.:47), (SEQ. ID. NO.:48), (SEQ. ID. NO.:49), (SEQ. ID. NO.:50), (SEQ. ID. NO.:51), (SEQ. ID. NO.:52), (SEQ. ID. NO.:53), (SEQ. ID. NO.:54), (SEQ. ID. NO.:55), (SEQ. ID. NO.:56), (SEQ. ID. NO.:57), (SEQ. ID. NO.:58), (SEQ. ID. NO.:59), (SEQ. ID. NO.:60), (SEQ. ID. NO.:61), (SEQ. ID. NO.:62), (SEQ. ID. NO.:63), (SEQ. ID. NO.:64), (SEQ. ID. NO.:65), (SEQ. ID. NO.:66), (SEQ. ID. NO.:67), (SEQ. ID. NO.:68), (SEQ. ID. NO.:69), (SEQ. ID. NO.:70), (SEQ. ID. NO.:71), (SEQ. ID. NO.:72), (SEQ. ID. NO.:73), (SEQ. ID. NO.:74), (SEQ. ID. NO.:75), (SEQ. ID. NO.:76), (SEQ. ID. NO.:77), (SEQ. ID. NO.:78), or (SEQ. ID. NO.:79), or comprising a sequence overlapping the sequence of any of these with respect to its position on the Nickerson reference sequence. 11. The method of claim 5, wherein said oligonucleotide primer is labeled with a fluorescent dye. 12. The method of claim 11, wherein said dye is SYBR Green I, YO-PRO-1, thiazole orange, Hex, pico green, edans, fluorescein, FAM, or TET. 13. A method of detecting a genetic predisposition for non-responsiveness to lovastatin, pravastatin, or simvastatin treatment in a human subject with coronary artery disease, comprising: a) collecting a tissue sample from a human subject; b) amplifying nucleic acids comprising the HindIII restriction site in intron 8 of the human lipoprotein lipase gene (LPL) from said tissue sample to obtain amplification products; and c) analyzing the amplification products for the absence of a HindIII recognition site in intron 8 of the human lipoprotein lipase gene, homozygosity for an absence of said HindIII recognition site indicating a genetic predisposition in said human subject for non-responsiveness to lovastatin, pravastatin, or simvastatin treatment for coronary artery disease. 14. The method of claim 13, wherein the tissue sample is a blood sample. 15. The method of claim 13, further comprising restricting the amplification products with a restriction enzyme before analyzing the amplification products. 16. The method of claim 13, wherein the restriction enzyme is HindIII. 17. The method of claim 13, wherein an oligonucleotide primer is used in amplifying said nucleic acids. 18. The method of claim 13, comprising the sequence GCA TCT GCC TTC AGC TAG ACA TTG (SEQ. ID. NO. 1) is used in amplifying said nucleic acids. 19. The method of claim 13, wherein an oligonucleotide primer comprising the sequence TCT TCC AGA AGG GTG AGA TTC CAA (SEQ. ID. NO. 2) is used in amplifying said nucleic acids. 20. The method of claim 13, wherein an oligonucleotide primer having the sequence GCA TCT GCC TTC AGC TAG ACA TTG (SEQ. ID. NO. 1) or TCT TCC AGA AGG GTG AGA TTC CAA (SEQ. ID. NO. 2) is used in amplifying said nucleic acids. 21. The method of claim 13, wherein a reverse oligonucleotide primer having the sequence 5'-GCA TCT GCC TTC AGC TAG ACA TTG-3' (SEQ. ID. NO. 1) and a forward oligonucleotide primer having the sequence 5'-TCT TCC AGA AGG GTG AGA TTC CAA-3' (SEQ. ID. NO. 2) are used in amplifying said nucleic acids. 22. The method of claim 13, wherein an oligonucleotide primer is used in amplifying said nucleic acids, said primer comprising a nucleotide sequence of (SEQ. ID. NO.:1), (SEQ. ID. NO.:2), (SEQ. ID. NO.:3), (SEQ. ID. NO.:4), (SEQ. ID. NO.:5), (SEQ. ID. NO.:6), (SEQ. ID. NO.:7), (SEQ. ID. NO.:8), (SEQ. ID. NO.:9), (SEQ. ID. NO.:10), (SEQ. ID. NO.:11), (SEQ. ID. NO.:12), (SEQ. ID. NO.:13), (SEQ. ID. NO.:14), (SEQ. ID. NO.:15), (SEQ. ID. NO.:16), (SEQ. ID. NO.:17), (SEQ. ID. NO.:18), (SEQ. ID. NO.:19), (SEQ. ID. NO.:20), (SEQ. ID. NO.:21), (SEQ. ID. NO.22), (SEQ. ID. NO.:23), (SEQ. ID. NO.:24), (SEQ. ID. NO.:25), (SEQ. ID. NO.:26), (SEQ. ID. NO.27), (SEQ. ID. NO.:28), (SEQ. ID. NO.:29), (SEQ. ID. NO.:30), (SEQ. ID. NO.:31), (SEQ. ID. NO.:32), (SEQ. ID. NO.:35), (SEQ. ID. NO.:36), (SEQ. ID. NO.:37), (SEQ. ID. NO.:38), (SEQ. ID. NO.:39), (SEQ. ID. NO.:40), (SEQ. ID. NO.:41), (SEQ. ID. NO.:42), (SEQ. ID. NO.:43), (SEQ. ID. NO.:44), (SEQ. ID. NO.:45),(SEQ. ID. NO.:46), (SEQ. ID. NO.:47), (SEQ. ID. NO.:48), (SEQ. ID. NO.:49), (SEQ. ID. NO.:50), (SEQ. ID. NO.:51), (SEQ. ID. NO.:52), (SEQ. ID. NO.:53), (SEQ. ID. NO.:54), (SEQ. ID. NO.:55), (SEQ. ID. NO.:56), (SEQ. ID. NO.:57), (SEQ. ID. NO.:58), (SEQ. ID. NO.:59), (SEQ. ID. NO.:60), (SEQ. ID. NO.:61), (SEQ. ID. NO.:62), (SEQ. ID. NO.:63), (SEQ. ID. NO.:64), (SEQ. ID. NO.:65), (SEQ. ID. NO.:66), (SEQ. ID. NO.:67), (SEQ. ID. NO.:68), (SEQ. ID. NO.:69), (SEQ. ID. NO.:70), (SEQ. ID. NO.:71), (SEQ. ID. NO.:72), (SEQ. ID. NO.:73), (SEQ. ID. NO.:74), (SEQ. ID. NO.:75), (SEQ. ID. NO.:76), (SEQ. ID. NO.:77), (SEQ. ID. NO.:78), or (SEQ. ID. NO.:79), or comprising a sequence overlapping the sequence of any of these with respect to its position on the Nickerson reference sequence. 23. The method of claim 13, wherein said oligonucleotide primer is labeled with a fluorescent dye. 24. The method of claim 23, wherein said dye is SYBR Green I, YO-PRO-1, thiazole orange, Hex, pico green, edans, fluorescein, FAM, or TET. 25. The method of claim 13, wherein said human subject is a coronary artery bypass graft patient. 26. A method of detecting genetic predisposition in a CABG patient for non-responsiveness to treatment with a statin drug selected from the group consisting of lovastatin, pravastatin, and simvastatin, comprising: a) collecting a tissue sample from a CABG patient; b) amplifying nucleic acids comprising the locus of the HindIII recognition site in intron 8 of the human lipoprotein lipase (LPL) gene from said blood sample to obtain amplification products; and c) analyzing the amplification products for the absence of the HindIII recognition site in intron 8 of the human lipoprotein lipase gene, homozygosity for an absence of said HindIII recognition site indicating a genetic predisposition in said CABG patient for non-responsiveness to treatment for coronary artery disease with a statin drug selected from the group consisting of lovastatin, pravastatin, and simvastatin. 27. The method of claim 26, wherein said tissue sample is a blood sample. 28. The method of claim 26, further comprising restricting the amplification products with a restriction enzyme before analyzing the amplification products. 29. The method of claim 28, wherein the restriction enzyme is HindIII. 30. The method of claim 26, wherein an oligonucleotide primer is used in amplifying said nucleic acids. 31. The method of claim 26, comprising the sequence GCA TCT GCC TTC AGC TAG ACA TTG (SEQ. ID. NO. 1) is used in amplifying said nucleic acids. 32. The method of claim 26, wherein an oligonucleotide primer comprising the sequence TCT TCC AGA AGG GTG AGA TTC CAA (SEQ. ID. NO. 2) is used in amplifying said nucleic acids. 33. The method of claim 26, wherein an oligonucleotide primer having the sequence GCA TCT GCC TTC AGC TAG ACA TTG (SEQ. ID. NO. 1) or TCT TCC AGA AGG GTG AGA TTC CAA (SEQ. ID. NO. 2) is used in amplifying said nucleic acids. 34. The method of claim 26, wherein a reverse oligonucleotide primer having the sequence 5'-GCA TCT GCC TTC AGC TAG ACA TTG-3' (SEQ. ID. NO. 1) and a forward oligonucleotide primer having the sequence 5'-TCT TCC AGA AGG GTG AGA TTC CAA-3' (SEQ. ID. NO. 2) are used in amplifying said nucleic acids. 35. The method of claim 26, wherein an oligonucleotide primer is used in amplifying said nucleic acids, said primer comprising a sequence of (SEQ. ID. NO.:1), (SEQ. ID. NO.:2), (SEQ. ID. NO.:3), (SEQ. ID. NO.:4), (SEQ. ID. NO.:5), (SEQ. ID. NO.:6), (SEQ. ID. NO.:7), (SEQ. ID. NO.:8), (SEQ. ID. NO.:9), (SEQ. ID. NO.:10), (SEQ. ID. NO.:11), (SEQ. ID. NO.:12), (SEQ. ID. NO.:13), (SEQ. ID. NO.:14), (SEQ. ID. NO.:15), (SEQ. ID. NO.:16), (SEQ. ID. NO.:17), (SEQ. ID. NO.:18), (SEQ. ID. NO.:19), (SEQ. ID. NO.:20), (SEQ. ID. NO.:21), (SEQ. ID. NO.:22), (SEQ. ID. NO.:23), (SEQ. ID. NO.:24), (SEQ. ID. NO.:25), (SEQ. ID. NO.:26), (SEQ. ID. NO.:27), (SEQ. ID. NO.:28), (SEQ. ID. NO.:29), (SEQ. ID. NO.:30), (SEQ. ID. NO.:31), (SEQ. ID. NO.:32), (SEQ. ID. NO.:35), (SEQ. ID. NO.:36), (SEQ. ID. NO.:37), (SEQ. ID. NO.:38), (SEQ. ID. NO.:39), (SEQ. ID. NO.:40), (SEQ. ID. NO.:41), (SEQ. ID. NO.:42), (SEQ. ID. NO.:43), (SEQ. ID. NO.:44), (SEQ. ID. NO.:45),(SEQ. ID. NO.:46), (SEQ. ID. NO.:47), (SEQ. ID. NO.:48), (SEQ. ID. NO.:49), (SEQ. ID. NO.:50), (SEQ. ID. NO.:51), (SEQ. ID. NO.:52), (SEQ. ID. NO.:53), (SEQ. ID. NO.:54), (SEQ. ID. NO.:55), (SEQ. ID. NO.:56), (SEQ. ID. NO.:57), (SEQ. ID. NO.:58), (SEQ. ID. NO.:59), (SEQ. ID. NO.:60), (SEQ. ID. NO.:61), (SEQ. ID. NO.:62), (SEQ. ID. NO.:63), (SEQ. ID. NO.:64), (SEQ. ID. NO.:65), (SEQ. ID. NO.:66), (SEQ. ID. NO.:67), (SEQ. ID. NO.:68), (SEQ. ID. NO.:69), (SEQ. ID. NO.:70), (SEQ. ID. NO.:71), (SEQ. ID. NO.:72), (SEQ. ID. NO.:73), (SEQ. ID. NO.:74), (SEQ. ID. NO.:75), (SEQ. ID. NO.:76), (SEQ. ID. NO.:77), (SEQ. ID. NO.:78), or (SEQ. ID. NO.:79), or comprising a sequence overlapping the sequence of any of these with respect to its position on the Nickerson reference sequence. 36. The method of claim 30, wherein said oligonucleotide primer is labeled with a fluorescent dye. 37. The method of claim 36, wherein said dye is SYBR Green I, YO-PRO-1, thiazole orange, Hex, pico green, edans, fluorescein, FAM, or TET. 38. A method of detecting genetic predisposition in a CABG for non-responsiveness to treatment with lovastatin, pravastatin, or simvastatin, comprising: a) collecting a tissue sample from a CABG patient, b) amplifying nucleic acids comprising the normal locus of the HindIII recognition site in intron 8 of the human lipoprotein lipase (LPL) gene from said blood sample to obtain amplification products, by using a reverse oligonucleotide primer having the sequence 5'-GCA TCT GCC TTC AGC TAG ACA TTG-3' (SEQ. ID. NO. 1) and a forward oligonucleotide primer having the sequence 5'-TCT TCC AGA AGG GTG AGA TTC CAA-3' (SEQ. ID. NO. 2); and c) analyzing the amplification products for the absence of the HindIII recognition site in intron 8 of the human lipoprotein lipase gene, homozygosity for an absence of said HindIII recognition site indicating a genetic predisposition in said CABG patient for non-responsiveness to treatment for coronary artery disease with lovastatin, pravastatin, or simvastatin. 39. The method of claim 38, wherein said tissue sample is a blood sample. 40. The method of claim 38, wherein said oligonucleotide primer is labeled with a fluorescent dye. 41. The method of claim 40, wherein said dye is SYBR Green I, YO-PRO-1, thiazole orange, Hex, pico green, edans, fluorescein, FAM, or TET. 42. A method of detecting in a human subject a genetic predisposition for non-responsiveness to treatment for coronary artery disease with a statin drug selected from the group consisting of lovastatin, pravastatin, or simvastatin, said method comprising: a) collecting a tissue sample from a human subject; b) amplifying nucleic acids comprising the normal locus of the HindIII recognition site in intron 8 of the human lipoprotein lipase (LPL) gene from said blood sample to obtain amplification products, by using a reverse oligonucleotide primer having the sequence 5'-GCA TCT GCC TTC AGC TAG ACA TTG-3' (SEQ. ID. NO. 1) and a forward oligonucleotide primer having the sequence 5'-TCT TCC AGA AGG GTG AGA TTC CAA-3' (SEQ. ID. NO. 2); c) restricting said amplification products with HindIII; and d) analyzing the restriction fragments for the absence of the HindIII recognition site in intron 8 of the human lipoprotein lipase gene, wherein homozygosity for an absence of said HindIII recognition site indicates a genetic predisposition for non-responsiveness to treatment for coronary artery disease with a statin drug selected from the group consisting of lovastatin, pravastatin, or simvastatin. 43. The method of claim 42, wherein the tissue sample is a blood sample. 44. The method of claim 42, wherein said oligonucleotide primer is labeled with a fluorescent dye. 45. The method of claim 42, wherein said dye is SYBR Green I, YO-PRO-1, thiazole orange, Hex, pico green, edans, fluorescein, FAM, or TET. 46. A method of detecting a genetic predisposition in a human subject for non-responsiveness to treatment with a statin drug selected from the group consisting of lovastatin, pravastatin, or simvastatin, said method comprising: a) collecting a tissue sample from a human subject; b) amplifying nucleic acids comprising the normal locus of the (TTTA).sub.n tetranucleotide repeat sequence in intron 6 of the human lipoprotein lipase gene (LPL) from said tissue sample to obtain amplification products; and c) analyzing the amplification products for (TTTA).sub.n tetranucleotide repeat alleles present in said nucleic acids, homozygosity for a (TTTA).sub.n 4 allele indicating a genetic predisposition for non-responsiveness to treatment for coronary artery disease with a statin drug selected from the group consisting of lovastatin, pravastatin, or simvastatin. 47. The method of claim 46, wherein the tissue sample is a blood sample. 48. The method of claim 46, wherein an oligonucleotide primer is used in amplifying said nucleic acids. 49. The method of claim 46, comprising the sequence 5'-CCT GGG TAA CTG AGC GAG ACT GTG TC-3' (SEQ. ID. NO.:33) is used in amplifying said nucleic acids. 50. The method of claim 46, wherein an oligonucleotide primer comprising the sequence 5'- ATC TGA CCA AGG ATA GTG GGA TAT A-3' (SEQ. ID. NO.:34) is used in amplifying said nucleic acids. 51. The method of claim 46, wherein an oligonucleotide primer having the sequence 5'-CCT GGG TAA CTG AGC GAG ACT GTG TC-3' (SEQ. ID. NO.:33) or 5'-ATC TGA CCA AGG ATA GTG GGA TAT A-3' (SEQ. ID. NO.:34) is used in amplifying said nucleic acids. 52. The method of claim 46, wherein a reverse oligonucleotide primer having the sequence 5'-CCT GGG TAA CTG AGC GAG ACT GTG TC-3' (SEQ. ID. NO.:33) and a forward oligonucleotide primer having the sequence 5'-ATC TGA CCA AGG ATA GTG GGA TAT A-3' (SEQ. ID. NO.:34) are used in amplifying said nucleic acids. 53. The method of claim 46, wherein amplifying said nucleic acids is done using an oligonucleotide primer comprising a nucleotide sequence of (SEQ. ID. NO.:33), (SEQ. ID. NO.:34), (SEQ. ID. NO.:82), (SEQ. ID. NO.:83), (SEQ. ID. NO.:84), (SEQ. ID. NO.:85), (SEQ. ID. NO.:86), (SEQ. ID. NO.:87), (SEQ. ID. NO.:88), (SEQ. ID. NO.:89), (SEQ. ID. NO.:90), (SEQ. ID. NO.:91), or (SEQ. ID. NO.:92), or comprising a sequence overlapping the sequence of any of these with respect to its position on the Nickerson reference sequence. 54. The method of claim 53, wherein said oligonucleotide primer is labeled with a fluorescent dye. 55. The method of claim 54, wherein said dye is SYBR Green I, YO-PRO-1, thiazole orange, Hex, pico green, edans, fluorescein, FAM, or TET.

Details for Patent 6,297,014

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-02-26