You’re using a public version of DrugPatentWatch with 5 free searches available | Register to unlock more free searches. CREATE FREE ACCOUNT

Last Updated: April 25, 2024

Claims for Patent: 6,274,703

✉ Email this page to a colleague

« Back to Dashboard

Summary for Patent: 6,274,703

Title:	Method of cell surface activation and inhibition
Abstract:	Disclosed are a peptide and its use in a method of screening test compounds as potential inhibitors of matrix metalloproteinases. The peptide consists of residues Trp 574 to Asp 656 in the TIMP-2 binding site of the C-terminal domain of gelatinase-A as shown by SEQ ID NO:19. The method comprises determining the inhibitory effect of a test compound in a competitive inhibition assay with said peptide in which a Ki/Kd=>1 is deemed an inhibitory effect of said test compound, and in which Ki is the inhibitor constant of said test compound and Kd is the dissociation constant of said peptide.
Inventor(s):	Goldberg; Gregory I. (St. Louis, MO)
Assignee:	Washington University (St. Louis, MO)
Application Number:	09/138,721
Patent Claims:	1. A target area for screening test compounds as inhibitors of matrix metalloproteinases consisting of a peptide of residues Trp.sup.574 to Asp.sup.656 in the tissue inhibitor of 72 kDa type IV collagenase binding site of the C-terminal domain of gelatinase-A as shown in SEQ ID NO:19. 2. A method of screening a test compound for inhibition of matrix metalloproteineases comprising: a) contacting said test compound and said metalloproteinease, and b) determining the inhibitory effect of said test compound in a competitive inhibition assay in which displacement of .sup.125 I-labeled peptide bound to the tissue inhibitor of 72 kDa type IV collagenase by unlabeled test compound and unlabeled peptide is measured and the K.sub.i /K.sub.d ratio is determined; wherein K.sub.i /K.sub.d.gtoreq.1 indicates inhibition, K.sub.i is the inhibitory constant of said test compound, K.sub.d is the dissociation constant of said peptide, and wherein said peptide consists of residues Trp.sup.574 to Asp.sup.656 in the tissue inhibitor of 72 kDa type IV collagenase binding site of the C-terminal domain of gelatinase A as shown in SEQ ID NO. 19.

Details for Patent 6,274,703

Glossary

Subscribe to access the full database, or Try a Trial
Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965	⤷ Try a Trial	2016-09-17
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

Make Better Decisions: Try a trial or see plans & pricing

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.