Claims for Patent: 6,274,367

Summary for Patent: 6,274,367

Title:	DNA coding for mammalian L-asparaginase
Abstract:	Disclosed is a DNA coding for mammalian L-asparaginase. Transformants introduced with the DNA effectively produce desired amounts of mammalian L-asparaginase such as those from humans, guinea pigs, and mice. The DNA is also useful as a probe for screening a DNA coding for desired mammalian L-asparaginase.
Inventor(s):	Ario; Takeshi (Okayama, JP), Taniai; Madoka (Okayama, JP), Torigoe; Kakuji (Okayama, JP), Kurimoto; Masashi (Okayama, JP)
Assignee:	Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo (Okayama, JP)
Application Number:	09/309,592
Patent Claims:	1. An isolated DNA encoding for mammalian L-asparaginase, which comprises nucleotide sequences encoding for either or both the amino acid sequences of SEQ ID NOs:1 and 2, and which is hybridizable to any of the nucleotide sequences of SEQ ID NOs:3 to 5 at 42.degree. C. in a solution of 5 .times.SSPE, 5.times.Denhardts solution, 0.5 w/v% SDS, and 100 .mu.g/ml denatured salmon sperm DNA, wherein said mammalian L-asparaginase has an amino acid sequence having at least 70% of its residues identical to the corresponding residues of the amino acid sequence of SEQ ID NO:7. 2. The isolated DNA according to claim 1, which comprises a nucleotide sequence encoding an amino acid sequence selected from the group consisting of SEQ ID NOs:6 to 8. 3. The isolated DNA according to claim 1, wherein, based on the degeneracy of the genetic code, one or more nucleotides in a nucleotide sequence selected from the group consisting of SEQ ID NOs:3, 4, 5, 9 and 10 are replaced with different nucleotides without altering the encoded amino acid sequences. 4. The isolated DNA according to claim 1, which encodes a human, guinea pig or mouse L-asparaginase. 5. A recombinant vector comprising the isolated DNA of claim 1. 6. A host cell transformed with the recombinant vector of claim 5. 7. A method for producing a mammalian L-asparaginase, comprising the steps of: transforming a host cell with a DNA in accordance with claim 1 in a manner such that said transformed host cell can produce a mammalian L-asparaginase intracellularly or extracelluarly when cultured; culturing said transformed host cell to produce said mammalian L-asparaginase; and recovering said produced mammalian L-asparaginase.

Details for Patent 6,274,367

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2015-02-08