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Last Updated: March 28, 2024

Claims for Patent: 6,232,122


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Summary for Patent: 6,232,122
Title: Inhibition of gene expression
Abstract:A method of inhibiting gene expression in is described. The method, which affects enzymatic activity in a plant, involves expressing in a plant, or a cell, cell line, tissue, or an organ thereof, a nucleotide sequence wherein the nucleotide sequence codes for an intron, or an intron fragment, of an SBE gene in a sense orientation; and wherein the nucleotide sequence does not contain a sequence that is sense to an exon sequence normally associated with the intron.
Inventor(s): Poulsen; Peter (Copenhagen, DK)
Assignee: Danisco A/S (Copenhagen, DK)
Application Number:08/983,440
Patent Claims:1. A method of selectively affecting starch branching enzymatic (SBE) activity in a starch producing organism comprising expressing in the starch producing organism a nucleotide sequence wherein the nucleotide sequence codes for an intron, or an intron fragment, of an SBE gene in a sense orientation; and wherein the nucleotide sequence does not contain a sequence that is sense to an exon sequence normally associated with the intron.

2. The method according to claim 1, wherein the starch producing organism comprises a biological sample derived therefrom, selected from the group consisting of a cell, cell line, tissue and organ.

3. The method according to claim 1, wherein the levels of amylopectin are changed.

4. The method according to claim 1, wherein the composition of starch is changed in the starch producing organism.

5. The method according to claim 1, wherein the starch producing organism is a plant.

6. The method according to claim 1, wherein the nucleotide sequence does not contain a sequence that is sense to an exon sequence.

7. The method according to claim 1, wherein the enzymatic activity is reduced.

8. The method according to claim 1, wherein the nucleotide sequence codes for at least a portion of one intron fragment in a sense orientation.

9. The method according to claim 1, wherein the nucleotide sequence codes for at least one entire one intron in a sense orientation.

10. A method of affecting starch branching enzymatic (SBE) activity in a starch producing organism comprising expressing in the starch producing organism a nucleotide sequence wherein the nucleotide sequence codes for an intron, or an intron fragment, of an SBE gene in a sense orientation; wherein the nucleotide sequence does not contain a sequence that is sense to an exon sequence normally associated with the intron; and wherein the nucleotide sequence comprises a sequence as shown as any one of SEQ.ID. No. 1 to SEQ.ID. No. 13 or a variant, derivative or homologue thereof, including combinations thereof.

11. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as any one of SEQ.ID. No. 1 to SEQ.ID. No. 13 or combinations thereof.

12. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as any one of SEQ.ID. No. 1 to SEQ.ID. No. 13.

13. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 1.

14. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 2.

15. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 3.

16. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 4.

17. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 5.

18. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 6.

19. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 7.

20. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 8.

21. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 9.

22. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 10.

23. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 11.

24. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 12.

25. The method of claim 10 wherein the nucleotide sequence comprises a sequence as shown as SEQ.ID. No. 13.

26. The method according to claim 10, wherein the starch producing organism comprises a biological sample derived therefrom, selected from the group consisting of a cell, cell line, tissue or organ.

27. The method according to claim 10, wherein the levels of amylopectin are changed.

28. The method according to claim 10, wherein the composition of starch is changed in the starch producing organism.

29. An isolated nucleic acid molecule encoding an intron or an intron fragment, of an starch branching enzymatic (SBE) gene in a sense orientation comprising a sequence as shown as any one of SEQ.ID. No. 1 to SEQ.ID. No. 13 or a variant, derivative or homologue thereof.

30. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as any one of SEQ.ID. No. 1 to SEQ.ID. No. 13.

31. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 1.

32. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 2.

33. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 3.

34. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 4.

35. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 5.

36. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.I.D. No. 6.

37. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No.7.

38. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 8.

39. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 9.

40. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No.10.

41. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No.11.

42. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No. 12.

43. The isolated nucleic acid molecule of claim 28 wherein comprising a sequence as shown as SEQ.ID. No.13.

44. A method of affecting starch branching enzymatic (SBE) activity in a starch producing organism comprising expressing in the starch producing organism a nucleotide sequence wherein the nucleotide sequence codes, for an intron, or an intron fragment, of an SBE gene in a sense orientation; wherein the nucleotide sequence does not contain a sequence that is sense to an exon sequence normally associated with the intron; and wherein the nucleotide sequence is expressed by a promoter having a sequence shown as SEQ.ID. No.14 or a variant, derivative or homologue thereof.

45. The method of claim 44 wherein the promoter comprises a sequence as shown as SEQ.ID.No. 14.

46. The method according to claim 44, wherein the starch producing organism comprises a biological sample derived therefrom, selected from the group consisting of a cell, cell line, tissue or organ.

47. A promoter having a sequence shown as SEQ.ID. No. 14, or a variant, derivative or homologue thereof.

48. The promoter of claim 47 having a sequence as shown as SEQ.ID.No. 14.

49. An isolated nucleic acid molecule having a sequence shown as SEQ.ID. No. 14, or a variant, derivative or homologue thereof.

50. The isolated nucleic acid molecule of claim 47 having a sequence as shown as SEQ.ID.No. 14.

51. The promoter according to claim 45, in combination with a gene of interest ("GOI").

52. A construct comprising the isolated nucleic acid molecule according to claim 50.

53. A construct comprising the promoter according to claim 45.

54. The construct according to claim 53, further comprising a gene of interest ("GOI").

55. A vector comprising the isolated nucleic acid molecule according to claim 50.

56. A vector comprising the promoter according to claim 45.

57. The vector according to claim 55, further comprising a gene of interest ("GOI").

58. A biological sample, selected from the group consisting of a cell, cell line, tissue or organ, containing the isolated nucleic acid molecule according to claim 50.

59. A biological sample, selected from the group consisting of a cell, cell line, tissue or organ, containing the promoter according to claim 45.

60. The biological sample according to claim 59, further comprising a gene of interest ("GOI").

61. A transgenic starch producing organism comprising or expressing the isolated nucleic acid molecule according to claim 28.

62. A transgenic starch producing organism containing the promoter according to claim 47.

63. The transgenic starch producing organism according to claim 62, further comprising a gene of interest ("GOI").

64. The transgenic starch producing organism according to any one of claims 61, 62, or 63, wherein the organism is a plant.

65. A starch obtained from the method of any one of claims 1, 2, 3, 4, 5, 6, 7 or 8.

66. pBEA11 (NCIMB 40754).

67. An isolated nucleic acid molecule comprising an intron nucleotide sequence that is obtainable from X-SBE 3.2 (NCIMB 40751) or a variant, derivative or homologue thereof.

68. The isolated nucleic acid molecule of claim 48 comprising an intron nucleotide sequence obtainable from X-SBE 3.2 (NCIMB 40751).

69. An isolated nucleic acid molecule comprising an intron nucleotide sequence that is obtainable from A-SBE 3.4 (NCIMB 40752) or a variant, derivative or homologue thereof.

70. The isolated nucleic acid molecule of claim 69 comprising an intron nucleotide sequence obtainable from X-SBE 3.4 (NCIMB 40752).

Details for Patent 6,232,122

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2015-07-14
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2015-07-14
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2015-07-14
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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