Claims for Patent: 6,222,104

Summary for Patent: 6,222,104

Title:	Inbred maize line NP948
Abstract:	The present invention is drawn to a novel DNA construct comprising an expression cassette having a constitutive promoter which functions in plant cells operably linked to a maize alcohol dehydrogenase intron, a DNA sequence of a gene encoding-a Cry 1Ab protein, and a termninator functional in plants and optionally further comprising a second cassette including a promoter which functions in plants operably linked to a maize alcohol dehydrogenase intron, a DNA sequence of a gene encoding for phosphinothricin acetyl transferase, and a terminator functional in plants wherein the two cassettes are transcribed in the same direction. Also provided are transgenic plants, particularly maize plants, having such a construct stably incorporated into their genomes.
Inventor(s):	Mettler; Irvin J (Richmond, CA), Krier; Merl (Sun City, AZ), Mies; David (St. Joseph, IL)
Assignee:	Novartis AG (Basle, CH)
Application Number:	09/291,238
Patent Claims:	1. Seed of Maize inbred line NP948 having been deposited under ATCC Accession No.209406, further comprising a nucleic acid construct with a first expression cassette comprising in operable linkage: (i) a CAMV 35S promoter, wherein said promoter is the sequence described in SEQ ID NO: 1; (ii) a maize alcohol dehydrogenase intron, wherein said intron is the sequence described in SEQ ID NO: 2; (iii) a DNA molecule encoding an insecticidal Cry1Ab protein toxin, wherein said DNA molecule encoding an insecticidal Cry1Ab protein toxin is the sequence described in SEQ ID NO: 3; and (iv) a NOS terminator, wherein said terminator is the sequence described in SEQ ID NO: 4. 2. An isolated seed of claim 1. 3. The seed of claim 1, wherein the nucleic acid construct further comprises a second expression cassette comprising in operable linkage: (i) a CaMV 35S promoter, wherein said promoter is the sequence described in SEQ ID NO: 5; (ii) a maize alcohol dehydrogenase intron, wherein said intron is the sequence described in SEQ ID NO: 6; (iii) a DNA molecule encoding a phosphinothricin acetyl transferase, wherein said DNA molecule encoding a phosphinothricin acetyl transferase is the sequence described in SEQ ID NO: 7; and (iv) a NOS terminator, wherein said terminator is the sequence described in SEQ ID NO: 8. 4. A maize plant, and parts thereof, of inbred line NP948, seed of said line having been deposited under ATCC Accession No 209406, said maize plant further comprising a nucleic acid construct with a first expression cassette comprising in operable linkage: (i) a CaMV 35S promoter, wherein said promoter is the sequence described in SEQ ID NO: 1; (ii) a maize alcohol dehydrogenase intron, wherein said intron is the sequence described in SEQ ID NO: 2; (iii) a DNA molecule encoding an insecticidal Cry1Ab protein toxin, wherein said DNA molecule encoding an insecticidal Cry1Ab protein toxin is the sequence described in SEQ ID NO: 3; and (iv) a NOS terminator, wherein said terminator is the sequence described in SEQ ID NO: 4. 5. Pollen of the plant of claim 4. 6. An ovule of the plant of claim 4. 7. A maize plant, or parts thereof, having all the genotypic and phenotypic characteristics of a plant according to claim 4. 8. The plant of claim 4, wherein the nucleic acid construct further comprises a second expression cassette comprising in operable linkage: (i) a CaMV 35S promoter, wherein said promoter is the sequence described in SEQ ID NO: 5; (ii) a maize alcohol dehydrogenase intron, wherein said intron is the sequence described in SEQ ID NO: 6; (iii) a DNA molecule encoding a phosphinothricin acetyl transferase, wherein said DNA molecule encoding a phosphinothricin acetyl transferase is the sequence described in SEQ ID NO: 7; and (iv) a NOS terminator, wherein said terminator is the sequence described in SEQ ID NO: 8. 9. A method for producing maize seed comprising crossing a maize plant according to claim 4 with itself or another maize plant according to claim 4. 10. A method for producing hybrid maize seed comprising crossing a maize plant according to claim 4 with a different maize plant to form hybrid maize seed. 11. Hybrid seed produced by the method of claim 10. 12. Hybrid maize plant, or parts thereof, grown from the seed of claim 11.

Details for Patent 6,222,104

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2014-11-09
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2014-11-09
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2014-11-09