Claims for Patent: 6,166,302

Summary for Patent: 6,166,302

Title:	Modified Bacillus thuringiensis gene for lepidopteran control in plants
Abstract:	Synthetic DNA sequences which are optimized for expression in plants, particularly maize, and which encode a Bacillus thuringiensis protein that is toxic to specific insects are provided, along with methods for the engineering of any synthetic insecticidal gene in maize.
Inventor(s):	Merlo; Donald J. (Carmel, IN), Folkerts; Otto (Carmel, IN)
Assignee:	Dow AgroSciences LLC (Indianapolis, IN)
Application Number:	08/729,601
Patent Claims:	1. A plant optimized nucleotide sequence that encodes an insecticidal crystal protein (ICP), wherein the plant optimized nucleotide sequence is SEQ ID NO: 1. 2. A purified ICP gene having a nucleotide sequence according to SEQ ID NO:1. 3. The purified ICP gene according to claim 2, wherein the ICP gene is inserted into the vector pDAB 917 and has accession number NRRL B-30042. 4. A synthetic genetic construct that expresses in plant cells comprising in sequence 5' to 3': a promoter sequence that initiates transcription in plant cells; a translational enhancer sequence; a plant optimized nucleotide gene sequence encoding an insecticidal crystal protein (ICP) wherein the gene sequence is SEQ ID NO: 1; and a polyadenylation sequence, wherein said promoter sequence, translational enhancer sequence, plant optimized nucleotide sequence and polyadenylation sequence are operably linked. 5. A synthetic gene construct according to claim 4, wherein the promoter is selected from the group consisting of inducible promoters, constitutive promoters, temporal-regulated promoters, developmentally-regulated promoters, tissue-preferred promoters, and tissue-specific promoters. 6. A synthetic genetic construct according to claim 4, wherein the promoter is CaMV 35S. 7. A synthetic genetic construct according to claim 4, wherein the translational enhancer sequence is a maize intron. 8. A synthetic genetic construct according to claim 7, wherein the maize intron is intron 1 or intron 6 of Adh1.S. 9. A trangenic maize plant wherein cells of the plant have been transformed with a plant optimized nucleotide sequence encoding an insecticidal crystal protein (ICP), wherein the plant optimized nucleotide sequence is SEQ ID NO:1. 10. A plant seed having in its genome an inheritable synthetic gene, wherein the synthetic gene comprises a plant optimized nucleotide sequence encoding an insecticidal crystal protein (ICP), wherein the plant optimized nucleotide sequence is SEQ ID NO: 1.

Details for Patent 6,166,302

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2015-10-13
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2015-10-13
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2015-10-13