Claims for Patent: 6,084,153

Summary for Patent: 6,084,153

Title:	Plants having enhanced nitrogen assimilation/metabolism
Abstract:	A genetic construct is taught which contains a nitrogen assimilation and/or metabolism gene and a promoter for the gene which is inducible under conditions where it would be beneficial to take-up, store or use nitrogen. The promoter can be, for example, induced by the presence of nitrate or other form of nitrogen and will be induced by application of a nitrogenous fertilizer. The stress inducible promoter btg-26 is also taught. Promoter btg-26 is isolated from the Brassica turgor gene--26 and exhibits osmotic-stress induced expression.
Inventor(s):	Good; Allen G. (Edmonton, CA), Stroeher; Virginia L. (Calgary, CA), Muench; Douglas G. (Pullman, WA)
Assignee:	The Governors of the University of Alberta (Edmonton, CA)
Application Number:	08/599,968
Patent Claims:	1. An isolated Brassica turgor gene-26 promoter element consisting of nucleotides 1-365 of SEQ ID NO:1. 2. An isolated Brassica turgor gene-26 promoter element consisting of a fragment of nucleotides 1-365 of SEQ ID NO:1 which is inducible under osmotic stress conditions. 3. A genetic construct comprising a Brassica turgor gene-26 promoter element of claims 1 or 2 operably associated with a heterologous gene. 4. A genetic construct comprising a Brassica turgor gene-26 promoter element of claims 1 or 2 operably associated with a nitrogen assimilation/metabolism enzyme coding sequence. 5. The genetic construct of claim 4, wherein the nitrogen assimilation/metabolism enzyme is selected from the group consisting of: glutamine synthetase, asparagine synthetase, glutamate 2:oxogluturate aminotransferase, asparaginase, glutamate dehydrogenase, aspartate aminotransferase and alanine aminotransferase or active modified forms thereof. 6. A method for producing a plant cell containing a promoter which is inducible under osmotic stress conditions, comprising: transforming a plant cell with a genetic construct of claim 3 such that a plant cell containing a promoter which is inducible under osmotic stress conditions is produced. 7. A method for producing a plant regenerated from plant cells containing a promoter which is inducible under osmotic stress conditions, comprising regenerating a plant cell containing a promoter which is inducible under osmotic stress conditions, thereby producing a plant, wherein the plant cell was transformed with a genetic construct of claim 3. 8. A method for producing a plant cell containing a promoter which is inducible under osmotic stress conditions, comprising: transforming a plant cell with a genetic construct of claim 4 such that a plant cell containing a promoter which is inducible under osmotic stress conditions is produced. 9. A method for producing a plant regenerated from plant cells containing a promoter which is inducible under osmotic stress conditions, comprising regenerating a plant cell containing a promoter which is inducible under osmotic stress conditions, thereby producing a plant, wherein the plant cell was transformed with a genetic construct of claim 4. 10. A method for producing a plant cell containing a promoter which is inducible under osmotic stress conditions, comprising: transforming a plant cell with a genetic construct of claim 5 such that a plant cell containing a promoter which is inducible under osmotic stress conditions is produced. 11. A method for producing a plant regenerated from plant cells containing a promoter which is inducible under osmotic stress conditions, comprising regenerating a plant cell containing a promoter which is inducible under osmotic stress conditions, thereby producing a plant, wherein the plant cell was transformed with a genetic construct of claim 5.

Details for Patent 6,084,153

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2039-03-29