Claims for Patent: 5,972,352

Summary for Patent: 5,972,352

Title:	Cloning of mite allergens
Abstract:	Recombinant DNA expression vectors or cloning vehicles capable of expressing a polypeptide displaying the antigenicity of an allergen of house dust mites of the genus Dermatophagoides. A synthetic protein or polypeptide displaying the antigenicity of all or a portion of an allergen of house dust mites of the genus Dermatophagoides. Methods of diagnosing and treating such allergies.
Inventor(s):	Thomas; Wayne R. (Nedlands, Western Australia, 6009, AU), Stewart; Geoffrey A. (Leeming, Western Australia, 6153, AU), Turner; Keven J. (Dalkeith, Western Australia, 6009, AU), Simpson; Richard J. (Richmond, Victoria, 3121, AU), Chua; Kaw-Yan (National University of Singapore, 119074, SG)
Assignee:
Application Number:	08/162,722
Patent Claims:	1. An isolated protein allergen of Dermatophagoides pteronyssinus, Der p II, produced recombinantly in a host cell transformed with a nucleic acid comprising the nucleotide sequence shown in FIG. 7. 2. An isolated protein allergen of Dermatophagoides pteronyssinus, Der p II, produced recombinantly in a host cell transformed with a nucleic acid comprising nucleotide bases 72-506 of the nucleotide sequence shown in FIG. 7. 3. A therapeutic composition comprising an isolated protein of claim 1 or claim 2 and a pharmaceutically acceptable carrier. 4. A method of producing an isolated protein of Dermatophagoides pteronyssinus comprising the steps of: a) culturing a host cell transformed with a nucleic acid encoding a protein allergen of Dermatophagoides pteronyssinus, Der p II, comprising the amino acid sequence shown in FIG. 7, in an appropriate medium to produce a mixture of cells and medium containing said protein allergen; and b) purifying said mixture to produce isolated Der p II protein allergen. 5. The method of claim 4 wherein the nucleic acid comprises the nucleotide sequence shown in FIG. 7. 6. The method of claim 4 wherein the nucleic acid comprises nucleotides 72-506 of the nucleotide sequence shown in FIG. 7. 7. A diagnostic reagent comprising the isolated protein allergen of claim 1 or claim 2. 8. An isolated protein allergen of Dermatophagoides pteronyssinus, Der p II, produced recombinantly in a host cell transformed with a nucleic acid encoding the protein allergen, wherein the protein allergen comprises the amino acid sequence shown in FIG. 7. 9. An isolated protein allergen of Dermatophagoides pteronyssinus, Der p II, produced recombinantly in a host cell transformed with a nucleic acid encoding the protein allergen, wherein the protein allergen comprises amino acid residues 1-129 of the amino acid sequence shown in FIG. 7. 10. An isolated protein allergen capable of stimulating T cells specific for a protein allergen of Dermatophagoides pteronyssinus, Der p II, wherein the Der p II protein allergen has the amino acid sequence shown in FIG. 7. 11. A therapeutic composition comprising an isolated protein of any one of claims 8, 9 and 10 and a pharmaceutically acceptable carrier. 12. A diagnostic reagent comprising the isolated protein allergen of any one of claims 8, 9 and 10.

Details for Patent 5,972,352

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Greer Laboratories, Inc.	N/A	insects (whole body), mite dermatophagoides pteronyssinus	Injection	101835	09/15/1958		2016-10-26