Claims for Patent: 5,958,724

Summary for Patent: 5,958,724

Title:	Process for producing heme proteins
Abstract:	The present invention relates to methods for producing heterologous heme proteins extracellularly comprising transforming a filamentous fungus with a vector comprising a DNA sequence encoding the heterologous heme protein and a DNA sequence encoding a preregion permitting secretion of the expressed heme protein, and culturing the transformed filamentous fungus in a suitable culture medium to produce the heme protein.
Inventor(s):	Dalb.o slashed.ge; Henrik (Virum, DK), Jensen; Ejner Bech (Virum, DK), Welinder; Karen Gjesing (Virum, DK)
Assignee:	Novo Nordisk A/S (Bagsvaerd, DK)
Application Number:	08/858,933
Patent Claims:	1. A process for the extracellular production of a heterologous heme protein in a strain of Aspergillus sp., the process comprising: (a) transforming a suitable strain of an Aspergillus sp. with a recombinant DNA vector which comprises a DNA sequence encoding a heterologous heme protein, and a DNA sequence encoding a preregion permitting secretion of the expressed heme protein, and (b) culturing the transformed strain of Aspergillus sp. in a suitable culture medium under conditions conducive to the production of the heme protein. 2. A process according to claim 1, wherein the heme protein produced by the Aspergillus sp. in step (b) is subsequently recovered from the culture. 3. A process according to claim 1, wherein the Aspergillus sp. is Aspergillus oryzae. 4. A process according to claim 1, wherein the preregion is derived from a gene encoding an Aspergillus sp amylase. 5. A process according to claim 1, wherein the preregion is derived from the gene encoding A. oryzae TAKA amylase, A. niger neutral .alpha.-amylase, A. niger acid-stable .alpha.-amylase, A. niger glucoamylase, or the peroxidase produced by Coprinus cinereus or macrorhizus. 6. A process according to any of claims 1, wherein the heme protein is an oxidoreductase. 7. A process according to claim 6, wherein the oxidoreductase is a peroxidase or a haloperoxidase. 8. A process according to claim 7 wherein the DNA sequence encoding the peroxidase is derived from a Coprinus sp., in particular Coprinus macrorhizus or cinereus. 9. A process according to claim 1, wherein the culture medium comprises hemin or a heme-containing material. 10. A process according to claim 9, wherein said hemin is present in the medium at a concentration of between 1 and 1000 .mu.g/ml. 11. A process according to claim 9, wherein said heme-containing material is selected from the group consisting of hemoglobin and red blood cells.

Details for Patent 5,958,724

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	PANHEMATIN	hemin for injection	For Injection	101246	07/20/1983		2011-03-22