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Last Updated: April 20, 2024

Claims for Patent: 5,952,215

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Summary for Patent: 5,952,215

Title:	Enzyme composition for tissue dissociation
Abstract:	An enzyme composition and its use for isolating cells or cell clusters from tissue is disclosed. The composition includes two purified collagenase enzymes and an endoprotease. The ratio of the total FITC casein activity of the enzyme composition to the Wunsch units of activity of the masses of collagenase I and collagenase II is about 85:1 to about 3,900:1.
Inventor(s):	Dwulet; Francis E. (Greenwood, IN), Smith; Marilyn E. (McCordsville, IN)
Assignee:	Roche Diagnostics Corporation (Indianapolis, IN)
Application Number:	09/184,616
Patent Claims:	1. An enzyme composition prepared by combining defined masses of purified proteases comprising collagenase I and collagenase II from Clostridium histolyticum and an endoprotease, wherein the ratio of the mass of collagenase II to the mass of collagenase I plus the mass of the collagenase II in the composition is about 0.3 to about 0.6 and wherein the ratio of the total FITC casein activity of the enzyme composition to the total Wunsch units of activity of the masses of collagenase I and collagenase II in the composition is about 130:1 to about 1400:1. 2. The enzyme composition of claim 1 wherein the endoprotease is a neutral protease. 3. The enzyme composition of claim 2 wherein the neutral protease is selected from the group consisting of thermolysin, dispase and Clostridium histolyticum neutral protease. 4. The enzyme composition of claim 2 wherein the neutral protease is thermolysin. 5. The enzyme composition of claim 1 wherein the ratio of the mass of collagenase II to the mass of collagenase I plus the mass of the collagenase II in the composition is about 0.35 to about 0.45. 6. The enzyme composition of claim 1 wherein the composition is substantially free of endotoxins. 7. A method of preparing an enzyme composition adapted for isolating living cells from tissue, said method comprising the steps of combining known masses of chromatographically purified collagenase I and collagenase II from Clostridium histolyticum, wherein the ratio of the mass of collagenase II to the mass of collagenase I plus the mass of the collagenase II in the composition is about 0.3 to about 0.6, and adding an amount of an endoprotease sufficient to raise the total FITC casein activity of the enzyme composition to a level such that the ratio of the total FITC casein activity of the enzyme composition to the total Wunsch unit activity of the masses of purified collagenase I and collagenase II in the enzyme composition is about 130:1 to about 1,400:1. 8. The method of claim 7, wherein the endoprotease is a neutral protease. 9. The method of claim 8 wherein the neutral protease is selected from the group consisting of thermolysin, dispase, and Clostridium histolyticum neutral protease. 10. The method of claim 8 wherein the neutral protease is thermolysin. 11. The method of claim 10 wherein the ratio of the mass of collagenase II to the mass of collagenase I plus the mass of the collagenase II in the composition is about 0.35 to about 0.45.

Details for Patent 5,952,215

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965	⤷ Try a Trial	2016-12-06
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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