Claims for Patent: 5,859,340

Summary for Patent: 5,859,340

Title:	Recombinant gene coding for a protein having endochitinase activity
Abstract:	Recombinant gene coding for a protein having endochitinase activity or for a precursor thereof which comprises the sequence of SEQ ID NO:1 below: Gly Gly Asp Leu Gly Ser VaL Ile Ser Asn Ser Met Phe Asp Gln Met Leu Lys His Arg Asn Glu Asn Ser Cys Gln Gly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile Thr Ala Ala Arg Ser Phe Pro Gly, Phe Gly Thr Ser Gly Asp Ile Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe Ala Gln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser Ala Pro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu Arg Gly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser Ser Gln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly Arg Gly Pro Ile Gln Ile Ser His Asn Tyr Asn Tyr Gly Pro Cys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn Pro Asp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys Thr Ala Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys Pro Ser Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro Ser Ala Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe Gly Val Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys Gly Arg Gly Asn Asp Asn Arg Val Gln Asp Arg Ile Gly Phe Tyr Arg Arg Tyr Cys Gly Ile Leu Gly Val Ser Pro Gly Asp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly Asn Gly Leu Leu Val Asp Thr Met. Application: For obtaining plants resisting to pathogenic agents.
Inventor(s):	Dubois; Michel (Buc, FR), Grison; Rene (Escalquens, FR), Leguay; Jean-Jacques (Auzeville Tolosane, FR), Pignard; Annie (Roquettes, FR), Toppan; Alain (Cornebarrieu, FR)
Assignee:	Sanofi (Paris, FR) Elf Aquitaine (Courbevoie, FR)
Application Number:	08/475,427
Patent Claims:	1. An isolated, purified gene that comprises a first polynucleotide encoding a polypeptide that has endochitinase activity and that has the sequence (SEQ ID NO: 1). 2. An isolated, purified gene according to claim 1 wherein said gene further comprises a second polynucleotide encoding the polypeptide of the sequence (SEQ ID NO: 2) immediately upstream of said first polynucleotide. 3. An isolated, purified gene according to claim 1, wherein said gene further comprises a polynucleotide encoding a signal peptide upstream of the sequence (SEQ ID NO: 1). 4. An isolated, purified gene according to claim 2, wherein said gene further comprises a third polynucleotide encoding the polypeptide of the sequence (SEQ ID NO: 3) immediately upstream of said second polynucleotide. 5. An isolated, purified gene according to claim 1, wherein the region of said gene encoding said polypeptide comprises genomic or complementary DNA from the 5' portion of the coding sequence of a tomato endochitinase gene and genomic or complementary DNA from the 3' portion of the coding sequence of a tobacco endochitinase gene. 6. An isolated, purified gene according to claim 5, wherein said region comprises at least one intron. 7. An isolated, purified gene according to claim 5, wherein said region comprises the sequence (SEQ ID NO: 4). 8. An isolated, purified gene according to claim 7, further comprising a sequence containing the 35S promoter of cauliflower mosaic virus. 9. An isolated, purified gene according to claim 7, further comprising a sequence containing the nopaline synthase terminator of Agrobacterium tumefaciens. 10. A plant cell which is transformed by an isolated, purified gene that comprises a first polynucleotide encoding a polypeptide that has endochitinase activity and that has the sequence (SEQ ID NO: 1). 11. A plant cell according to claim 10, which belongs to one of the species Nicotiana tabacum, Helianthus annuus or Brassica napus. 12. A plant or plant part, which contains an isolated, purified gene that comprises a polynucleotide encoding a polypeptide that has endochitinase activity and that has the sequence (SEQ ID NO. 1). 13. A plant or plant part according to claim 12, which belongs to one of the species Nicotiana tabacum, Helianthus annuus or Brassica napus. 14. A plant part according to claim 12, which plant part is a seed. 15. A method for obtaining tobacco or rape plants resistant to chitin-containing pathogens, which comprises (i) transforming tobacco or rare plant cells with an isolated, purified gene which comprises a first polynucleotide encoding a polypeptide of the sequence (SEQ ID NO: 1), said polypeptide having endochitinase activity, (ii) multiplying said transformed cells, and (iii) regenerating said plants. 16. A method according to claim 15, which is applied to one of the species Nicotiana tabacum or Brassica napus. 17. A method for obtaining a polypeptide, said method comprising the steps of (i) culturing plant cells or calluses containing, in a context capable of permitting its expression, an isolated, purified gene that comprises a first polynucleotide encoding a polypeptide that has endochitinase activity and that has the sequence (SEQ ID NO: 1), (ii) lysing said cells or calluses, and (iii) isolating and purifying said polypeptide. 18. A method according to claim 17, wherein said plant cells or calluses are selected from one of the species Nicotiana tabacum, Helianthus annuus or Brassica napus.

Details for Patent 5,859,340

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2016-01-12
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2016-01-12
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2016-01-12