Claims for Patent: 5,767,379

Summary for Patent: 5,767,379

Title:	Commercial production of avidin in plants
Abstract:	A method for commercial production of avidin entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the avidin-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of avidin expression and that can be used as a site of integration for high level expression of other genes of interest.
Inventor(s):	Baszczynski; Chris (Urbandale, IA), Hood; Elizabeth (Clive, IA), Maddock; Sheila (Johnston, IA), Meyer; Terry EuClaire (Urbandale, IA), Register, III; James C. (Ames, IA), Witcher; Derrick (Urbandale, IA), Howard; John A. (Wes Des Moines, IA)
Assignee:	Howard; John (Des Moines, IA)
Application Number:	08/554,586
Patent Claims:	1. A transgenic plant stably transformed with a DNA molecule that comprises (i) a plant-compatible promoter, (ii) a signal sequence, and, (iii) a heterologous, avidin-encoding sequence that are operably linked, such that at least 0.1% of total extracted protein is avidin. 2. A transgenic plant according to claim 1, wherein said plant is a corn plant. 3. A transgenic plant according to claim 2, wherein said plant is of strain 70415, germplasm of which strain has been deposited under ATCC Accession No. 97328. 4. A transgenic plant according to claim 1, wherein said avidin-encoding sequence incorporates plant-preferred codons. 5. A transgenic plant according to claim 1, wherein said signal sequence is a peptide export sequence. 6. A transgenic plant according to claim 5, wherein said peptide export sequence is a barley alpha amylase peptide export sequence. 7. A transgenic plant according to claim 1, wherein said DNA molecule further comprises an intron sequence. 8. A transgenic plant according to claim 7, wherein said intron is an ubiquitin intron. 9. A transgenic plant according to claim 1, wherein said plant compatible promoters is the ubiquitin promoter. 10. A transgenic plant according to claim 1, wherein said total extracted protein is about 1% avidin. 11. Seed that is the product of a plant according to claim 1. 12. Seed that is the product of a plant according to claim 10.

Details for Patent 5,767,379

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-02-26