Claims for Patent: 5,767,368

Summary for Patent: 5,767,368

Title:	Method for producting a cereal plant with foreign DNA
Abstract:	A method for isolating a cereal plant with foreign DNA by bombarding meristem primordia tissue with particles coated with the foreign DNA in a culture media is described. The foreign DNA in the transformed plants can provide proteins which impart disease and/or insect resistance or other desirable properties.
Inventor(s):	Zhong; Heng (East Lansing, MI), Sticklen; Masomeh B. (Okemos, MI)
Assignee:	Board of Trustees operating Michigan State University (East Lansing, MI)
Application Number:	08/415,426
Patent Claims:	1. A method for producing a maize plant with foreign DNA encoding a herbicide or insecticide resistance which comprises: (a) providing isolated shoot apex meristem primordia of germinated embryos of the maize which have been separated from a coleoptile and exposed by removal of leaf primordia of the plant in a first culture medium comprising a cytokinin selected from the group consisting of N.sub.6 -benzyladenine, 6-furfurylamino-purine and 6-(4-hydroxy-3-methylbut-2-enylamino)purine at a concentration between about 0.001 to 10 .mu.M and optionally an auxin selected from the group consisting of 3,6-dichloro-o-anisic acid, 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, indole-3-butyric acid and .alpha.-naphthaleneacetic acid at a concentration of between about 0.001 to 10 .mu.M with frequent subculturing over time to enable the meristem primordia to be transformed with the foreign DNA; (b) bombarding the subcultured meristem primordia with micron sized particles coated with the foreign DNA to transform the meristem primordia with the DNA; (c) growing the bombarded meristem primordia in a second culture medium containing the auxin and optionally the cytokinin and selecting for transformed meristem primordia using the herbicide; and (d) regenerating the meristem primordia containing the foreign DNA in a culture medium containing the auxin to produce the transformed maize plant which has the herbicide or insect resistance. 2. The method of claim 1 wherein in addition the foreign DNA encodes an enzyme which is used as a visual selection marker. 3. The method of claim 2 wherein the enzyme is .beta.-glucuronidase. 4. The method of claim 1 wherein the first and second culture medium contains casein hydrolysate and N.sub.6 -benzyladenine and optionally 2,4-dichlorophenoxyacetic acid and the meristem primordia in step (a) are cultured in darkness for four weeks and then in the light for four weeks. 5. The method of claim 4 wherein the first culture medium and second culture medium contain MS medium. 6. The method of claim 5 wherein the first culture medium and second culture medium contain 0.5 to 1 g/liter casein hydrolysate and the 2,4-dichlorophenoxyacetic acid. 7. The method of claim 1 wherein the foreign DNA includes a promoter. 8. The method of claim 7 wherein the promoter is rice actin 1 promoter. 9. The method of claim 8 wherein the foreign DNA includes a 5' intron and a 5' non-coding exon. 10. The method of claim 1 wherein the foreign DNA encodes a bar gene for herbicide resistance. 11. The method of claim 1 wherein the foreign DNA encodes a pin 2 gene for insect resistance. 12. The method of claim 1 wherein the foreign DNA encodes a bar gene for resistance to glufosinate ammonium and a pin-2-gene for insect resistance. 13. The method of claim 1 wherein the foreign DNA is contained in plasmid pTW-a. 14. The method of claim 1 wherein the auxin in step (c) is indole-3-butyric acid (IBA). 15. The method of claim 14 wherein the indole-3-butyric acid is present in an amount between 0.001 and 10 .mu.M.

Details for Patent 5,767,368

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-02-26