Claims for Patent: 5,747,340
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Summary for Patent: 5,747,340
Title: | Targeted gene expression using preproendothelin-1 promoters |
Abstract: | This invention provides a vector for expression of a nucleic acid cassette in bronchial epithelial and vascular endothelial cells comprising a segment of the 5\'-flanking region of the preproendothelin-1 gene, upstream from the transcription start site, the first exon of the preproendothelin-1 gene, and a nucleic acid cassette, wherein the nucleic acid cassette is located within the first exon, in sequential and positional relationship for expression of the nucleic acid cassette. |
Inventor(s): | Harats; Dror (Ramat-Gan, IL), Kurihara; Hiroki (Toyko, JP), Belloni; Paula Nanette (Moss Beach, CA), Sigal; Charles Elliott (San Francisco, CA) |
Assignee: | Syntex (U.S.A.) Inc. (Palo Alto, CA) |
Application Number: | 08/395,742 |
Patent Claims: | 1. A vector for expression of a nucleic acid casette comprising a murine preproendothelin-1 gene 5'-flanking segment, the first exon of the preproendothelin-1 gene, and a nucleic acid
cassette, wherein the nucleic acid cassette is located within the first exon, in sequential and positional relationship for expression of the nucleic acid cassette.
2. A vector of claim 1 further comprising the first intron of the preproendothelin-1 gene, said intron located downstream from the first exon. 3. A vector of claim 2 comprising about 0.8 kb of the first intron. 4. A vector of claim 3 wherein the nucleic acid cassette is positioned between the noncoding and coding regions of said exon, in sequential and positional relationship for expression of the nucleic acid cassette. 5. A vector of claim 1 wherein the segment of the 5'-flanking region comprises about the first 0.45 kb upstream from the transcription start site. 6. A vector of claim 1 wherein the segment of the 5'-flanking region comprises about the first 1.4 kb upstream from the transcription start site. 7. A vector of claim 1 wherein the segment of the 5'-flanking region comprises about the first 5.9 kb upstream from the transcription start site. 8. A vector of claim 1 wherein the segment of the 5'-flanking region comprises about the first 9.1 kb upstream from the transcription start site. 9. A vector of claim 3 comprising 0.45 kb of the 5'-flanking region, the first exon, and about 0.8 kb of the first intron of the preproendothelin-1 gene. 10. A vector of claim 3 comprising 1.4 kb of the 5'-flanking region, the first exon, and about 0.8 kb of the first intron of the preproendothelin-1 gene. 11. A vector of claim 3 comprising 5.9 kb of the 5'-flanking region, the first exon, and about 0.8 kb of the first intron of the preproendothelin-1 gene. 12. A vector of claim 1 wherein the cassette comprises a nucleic acid sequence encoding a protein, a polypeptide, a ribozyme, or an antisense RNA. 13. The isolated genomic murine preproendothelin-1 5'-flanking region. 14. A plasmid comprising the 5'-flanking region of claim 13. 15. A method for expressing a gene of interest in vascular endothelial or bronchial epithelial cells which method comprises transfecting the cells with a vector of claim 1. |
Details for Patent 5,747,340
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | 06/04/1986 | ⤷ Try a Trial | 2015-05-05 |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | ⤷ Try a Trial | 2015-05-05 | |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | Injection | 103132 | ⤷ Try a Trial | 2015-05-05 | |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
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