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Last Updated: March 28, 2024

Claims for Patent: 5,681,725


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Summary for Patent: 5,681,725
Title: Process for producing heme proteins
Abstract:Process for producing an extracellular heme protein in increased yields, the process comprising culturing an apoprotein producing microorganism in a fermentation medium containing heme or a heme-containing material under conditions permitting the production of active, recombined heme protein, and recovering the resulting heme protein from the culture medium.
Inventor(s): Jensen; Ejner Bech (Virum, DK)
Assignee: Novo Nordisk A/S (Bagsvaerd, DK)
Application Number:08/284,566
Patent Claims:1. A process for producing an extracellular heme protein in increased yields, the process comprising:

(a) culturing an apoprotein producing microorganism in a fermentation medium containing heme or a heme-containing material under conditions where heme is incorporated into the apoprotein and;

(b) recovering the resulting heme protein from the medium.

2. A process according to claim 1, wherein the microorganism is transformed with a recombinant expression vector comprising a DNA sequence encoding a heme protein.

3. A process according to claim 2, wherein the DNA sequence encodes a heterologous heme protein.

4. A process according to claim 1, wherein the microorganism is a fungus.

5. A process according to claim 4, wherein the fungus is a filamentous fungus.

6. A process according to claim 5, wherein the filamentous fungus is a strain of Aspergillus.

7. A process according to any of claim 1, wherein the heme protein is an oxidoreductase or a catalase.

8. A process according to claim 7, wherein the oxidoreductase is a peroxidase or a haloperoxidase.

9. A process according to claim 8 wherein the peroxidase is derived from a species of Coprinus, e.g. Coprinus macrorhizus or Coprinus cinereus, or Arthrmyces.

10. A process according to any of claim 1, wherein the heme-containing material is hemoglobin or red blood cells.

11. A process according to claim 1, wherein the fermentation medium contains hemin in an amount of 1-1000 mg/l.

12. The process of claim 11 wherein the fermentation medium contains hemin in an amount of 10-100 mg/l.

13. A process according to claim 10, wherein the fermentation medium contains hemoglobin in an amount of 0.5-50 g/l.

14. The process according to claim 10, wherein the fermentation medium contains hemoglobin in the amount 1-25 g/l.

15. A process according to claim 10, wherein the fermentation medium contains red blood cells in an amount of 0.5-50 g/l.

16. A process according to claim 1, wherein the fermentation medium additionally contains a surface active agent.

17. A process according to claim 1, wherein the fermentation medium contains the surface-active agent in an amount of 1-100 ml/l.

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