Claims for Patent: 5,670,369

Summary for Patent: 5,670,369

Title:	Method for the production of soluble collagen
Abstract:	The invention includes an improved method for producing an aqueous solution of soluble collagen. The method comprises the steps of: (A) providing an aqueous ground slurry of insoluble collagen containing insoluble collagen at a first concentration and at a pH effective to obtain activity for a proteolytic enzyme added in step (B); (B) adding a proteolytic enzyme to said slurry; (C) reacting said slurry and enzyme at a temperature, T.sub.1, and for a time t.sub.1 effective for forming a slurry containing at least some soluble collagen; (D) diluting at least a portion of the slurry formed in step (C) with water to form a diluted slurry containing insoluble collagen at a second concentration; and (E) reacting said diluted slurry obtained in step (D) at a temperature, T.sub.2 and for a time t.sub.2, effective to produce a solution containing an increased amount of soluble collagen. The improved process of the present invention permits the preparation of soluble collagen utilizing reduced amounts of enzyme and water, and requires less time at low concentration for completion of the reaction.
Inventor(s):	Fink; David J. (Shaker Hts., OH), Virnelson; Kevin M. (Mayfield Hts., OH)
Assignee:	Ranpak Corporation (Concord, OH)
Application Number:	08/670,338
Patent Claims:	1. An improved method for producing an aqueous solution of soluble collagen comprising the steps of: (A) providing an aqueous ground slurry of insoluble collagen containing from about 2.0% to about 5.0% by weight of insoluble collagen at a pH effective to obtain activity for a proteolytic enzyme added in step (B); (B) adding a proteolytic enzyme to said slurry; (C) reacting said slurry and enzyme at a temperature, T.sub.1 and for a time t.sub.1 effective for forming a slurry containing at least some soluble collagen; (D) diluting at least a portion of the slurry formed in step (C) with water to form a diluted slurry containing from about 0.1% to about 1% by weight of insoluble collagen; and (E) reacting said diluted slurry obtained in step (D) at a temperature, T.sub.2 and for a time t.sub.2 effective to produce a solution containing an increased amount of soluble collagen. 2. The method of claim 1 wherein the aqueous ground slurry provided in (A) contains from about 2.0% to about 3.5% by weight of insoluble collagen. 3. The method of claim 1 wherein the aqueous ground slurry provided in (A) contains from about 3.0% to about 3.5% by weight of insoluble collagen. 4. The method of claim 1 wherein the slurry formed in step (D) contains from about 0.20% to about 0.40% by weight of insoluble collagen. 5. The method of claim 1 wherein temperatures T.sub.1 in step (C) and T.sub.2 in step (E) are each independently from about 5.degree. C. to about 35.degree. C. 6. The method of claim 1 wherein t.sub.1 is from about 12 hours to about 20 days. 7. The method of claim 1 wherein t.sub.1 is from about 12 to about 24 hours. 8. The method of claim 1 wherein the pH of the diluted slurry obtained in step (D) is adjusted to maintain activity for the proteolytic enzyme present in the diluted slurry. 9. The method of claim 8 wherein the pH is adjusted to between about 1.5 and about 3.0. 10. The method of claim 1 wherein the pH of the slurry provided in step (A) is between about 1.5 and 3.0. 11. The method of claim 1 wherein the proteolytic enzyme added in step (B) is an enzyme selected from the group consisting of porcine mucosal pepsin, bromelain, chymopapain, chymotrypsin, collagenase, ficin, papain, peptidase, proteinase A, proteinase K, trypsin, microbial proteases, and combinations of such enzymes. 12. The method of claim 1 wherein the reaction in (E) is terminated by rendering the proteolytic enzyme inactive by adjusting the pH of the diluted slurry; and/or reducing the temperature of said diluted slurry. 13. The method of claim 1 further comprising monitoring said reaction in step (E) and continuing the reaction until soluble collagen of a desired number average molecular weight is obtained. 14. The method of claim 1 further comprising monitoring said reaction in step (E) by measuring the solution viscosity of the diluted slurry at two different shear rates whereby said reaction is complete when a ratio of (viscosity at low shear)/(viscosity at high shear) is substantially maximized. 15. The method of claim 14 wherein said viscosity ratio is at least about 75% of the maximum attained. 16. The method of claim 1 further comprising monitoring said reaction in step (E) by measuring the concentration and the number average molecular weight ( Mn) of the soluble collagen whereby said reaction is considered complete when said concentration and said Mn are maximized. 17. The method of claim 1 wherein the soluble collagen obtained has a number average molecular weight of at least about 300,000 daltons. 18. An aqueous solution of solubilized collagen prepared in accordance with the method of claim 1. 19. An improved method for producing an aqueous solution of solubilized collagen comprising: (A) providing an aqueous ground slurry containing from about 2.0% to about 3.5% by weight of insoluble collagen at a pH sufficient to obtain activity for a proteolytic enzyme added in step (B); (B) adding said proteolytic enzyme to said slurry and reacting said enzyme with said insoluble collagen at a temperature T.sub.1, and for a time t.sub.1 to produce a slurry containing at least some soluble collagen; (C) diluting at least a portion of the slurry obtained in (B) with water to form a diluted slurry containing the proteolytic enzyme and from about 0.1% to about 1.0% by weight of insoluble collagen; (D) reacting said diluted slurry and enzyme at a temperature T.sub.2, and for a time t.sub.2, effective to produce a solution containing an increased amount of soluble collagen; and (E) withdrawing said solution of soluble collagen as product. 20. The method of claim 19 wherein the reaction in step (D) is controlled to obtain a solution having a high concentration of soluble collagen by measuring the concentration and Mn of soluble collagen, whereby said reaction is considered complete when said Mn is at least about 300,000 daltons and said concentration of said soluble collagen is maximized. 21. The method of claim 19 wherein the aqueous ground slurry provided in step (A) contains from about 3.0% to about 3.5% by weight of insoluble collagen. 22. The method of claim 19 wherein the pH of the aqueous ground slurry provided in step (A) is from about 1.5 to about 3. 23. The method of claim 19 wherein t.sub.1 is from about 12 hours to about 20 days. 24. The method of claim 19 wherein t.sub.2 is from about 6 to about 30 hours. 25. The method of claim 19 wherein the temperatures T.sub.1 and T.sub.2 are each independently within the range of from about 5.degree. C. to about 35.degree. C. 26. The method of claim 19 wherein the pH of the diluted slurry formed in step (C) is adjusted to be within the range of about 1:5 to about 3. 27. The method of claim 19 wherein the proteolytic enzyme added in step (B) is an enzyme selected from the group consisting of porcine mucosal pepsin, bromelain, chymopapain, chymotrypsin, collagenase, ficin, papain, peptidase, proteinase A, proteinase K, trypsin, microbial proteases, and combinations of such enzymes. 28. The method of claim 19 wherein the reaction in step (D) is terminated by rendering the proteolytic enzyme inactive by adjusting the pH of the diluted slurry andr reducing the temperature of the diluted slurry. 29. An aqueous solution of solubilized collagen prepared in accordance with the method of claim 19.

Details for Patent 5,670,369

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Discure Medical, Llc	CHYMODIACTIN	chymopapain	For Injection	018663	11/10/1982		2039-03-29
Discure Medical, Llc	CHYMODIACTIN	chymopapain	For Injection	018663	08/21/1984		2039-03-29
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2039-03-29