Claims for Patent: 5,646,042

Summary for Patent: 5,646,042

Title:	C-myb targeted ribozymes
Abstract:	Enzymatic nucleic acid molecules which cleave c-myb RNA or other RNAs associated with restenosis or cancer.
Inventor(s):	Stinchcomb; Dan T. (Boulder, CO), Draper; Kenneth (Boulder, CO), McSwiggen; James (Boulder, CO), Jarvis; Thale (Boulder, CO)
Assignee:	Ribozyme Pharmaceuticals, Inc. (Boulder, CO)
Application Number:	08/373,124
Patent Claims:	1. An enzymatic RNA molecule which specifically cleaves c-myb RNA. 2. An enzymatic RNA molecule of claim 1, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ. ID. NOS. 1-78, wherein said enzymatic RNA molecule is in a hepatitis delta virus motif. 3. The enzymatic RNA molecule of claim 1, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ. ID. NOS. 2, 4-6, 9-16, 18-20, 22-45, 47-98, wherein said enzymatic RNA molecule is in a hammerhead motif. 4. The enzymatic RNA molecule of claim 1, wherein said enzymatic RNA molecule is in a hammerhead motif. 5. The enzymatic RNA molecule of claim 1, wherein said enzymatic RNA molecule is in a hairpin, hepatitis delta virus, VS nucleic acid, group I intron, or RNAseP nucleic acid motif. 6. The enzymatic RNA molecule of claim 5, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ. ID. NOS. 99, 100 or 130-148, wherein said enzymatic RNA molecule is in a hairpin motif. 7. The enzymatic RNA molecule of claim 5 consisting of any sequence selected from the group of SEQ. ID NOS. 121, 122, 149-167. 8. The enzymatic RNA molecule of any of claims 1, 2, 3, 4, 5, 6 or 7, wherein said enzymatic RNA molecule comprises between 12 and 100 bases complementary to said mRNA. 9. The enzymatic RNA molecule of any of claims 1, 2, 3, 4, 5, 6 or 7, wherein said enzymatic RNA molecule comprises between 14 and 24 bases complementary to said mRNA. 10. An enzymatic RNA molecule consisting of any sequence selected from the group of SEQ. ID NOS. 101-120, 123-129, 149-167, and odd numbers from 171-1225. 11. The enzymatic RNA molecule of any of claims 3 or 4 or 10, wherein said enzymatic RNA molecule comprises at least five ribose residues, and wherein said enzymatic RNA molecule comprises phosphorothioate linkages at at least three 5' terminal nucleotides, and wherein said enzymatic RNA molecule comprises a 2'-C-allyl modification at position No. 4 of said enzymatic RNA molecule, and wherein said enzymatic RNA molecule comprises at least ten 2'-O-methyl modifications. 12. The enzymatic RNA molecule of any of claims 3 or 4 or 10, wherein said enzymatic RNA molecule comprises at least five ribose residues, and wherein said enzymatic RNA molecule comprises phosphorothioate linkages at at least three 5' terminal nucleotides, and wherein said enzymatic RNA molecule comprises a 2'-amino modification at position No. 4 and/or at position No. 7 of said nucleic acid, wherein said enzymatic RNA molecule comprises at least ten 2'-O-methyl modifications. 13. The enzymatic RNA molecule of any of claims 3 or 4 or 10, wherein said enzymatic RNA molecule comprises at least five ribose residues, and wherein said enzymatic RNA molecule comprises phosphorothioate linkages at at least three 5' terminal nucleotides, and wherein said enzymatic RNA molecule comprises abasic substitution at position No. 4 and/or at position No. 7 of said enzymatic RNA molecule, wherein said enzymatic RNA molecule comprises at least ten 2'-O-methyl modifications. 14. The enzymatic RNA molecule of any of claims 3 or 4 or 10, wherein said enzymatic RNA molecule comprises at least five ribose residues, and wherein said enzymatic RNA molecule comprises phosphorothioate linkages at at least three 5' terminal nucleotides, and wherein said enzymatic RNA molecule comprises 6-methyl uridine substitutions at position No. 4 and/or at position No. 7 of the said nucleic acid molecule, wherein said enzymatic RNA molecule comprises at least ten 2'-O-methyl modifications. 15. The enzymatic RNA molecule of any of claims 3, 4 or 10, wherein said nucleic acid molecule comprises a stem II region of length greater than or equal to two base-pairs. 16. The enzymatic RNA molecule in a hairpin motif of any of claims 5, 6 or 7, wherein said enzymatic RNA molecule comprises a stem IV region of length greater than or equal to two base-pairs. 17. The enzymatic RNA molecule of any of claims 5, 6 or 7, wherein said enzymatic RNA molecule comprises a stem II region of length between three and seven base-pairs. 18. The enzymatic nucleic acid of any one of claims 1, 2, 3, 4, 5, 6 or 7, wherein said nucleic acid comprises a 3'-3' linked inverted ribose moeity at the 3' end of said nucleic acid molecule. 19. The enzymatic RNA molecule of any one of claims 1, 2, 3, 4, 5, 6 or 7, wherein said enzymatic RNA molecule comprises a 3'-3' linked inverted thymidine moeity at the 3' end of said nucleic acid molecule. 20. The enzymatic RNA molecule of any one of claims 1, 2, 3, 4, 5, 6 or 7, wherein said enzymatic RNA molecule comprises a 2'-3' linked inverted ribose or thymidine moeity at the 3' end of said enzymatic RNA molecule. 21. The enzymatic RNA molecule of any one of claims 1, 2, 3, 4, 5, 6 or 7, wherein said enzymatic RNA molecule comprises a 5'-end modification. 22. The enzymatic RNA molecule of claim 11, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted ribose moeity at the 3' end of said enzymatic RNA molecule. 23. The enzymatic RNA molecule of any one of claim 11, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted thymidine moeity at the 3' end of said molecule. 24. The enzymatic RNA molecule of claim 11, wherein said enzymatic RNA molecule further comprises a 2'-3' linked inverted ribose or thymidine moeity at the 3' end of said enzymatic RNA molecule. 25. The enzymatic RNA molecule of claim 11, wherein said enzymatic RNA molecule further comprises a 5'-end modification. 26. The enzymatic RNA molecule of claim 12, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted ribose moeity at the 3' end of said enzymatic RNA molecule. 27. The enzymatic RNA molecule of claim 12, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted thymidine moeity at the 3' end of said enzymatic RNA molecule. 28. The enzymatic RNA molecule of claim 12, wherein said enzymatic RNA molecule further comprises a 2'-3' linked inverted ribose or thymidine moeity at the 3' end of said enzymatic RNA molecule. 29. The enzymatic RNA molecule of claim 12, wherein said enzymatic RNA molecule further comprises a 5'-end modification. 30. The enzymatic RNA molecule of claim 13, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted ribose moeity at the 3' end of said enzymatic RNA molecule. 31. The enzymatic RNA molecule of any one of claim 13, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted thymidine moeity at the 3' end of said enzymatic RNA molecule. 32. The enzymatic RNA molecule of claim 13, wherein said enzymatic RNA molecule further comprises a 2'-3' linked inverted ribose or thymidine moeity at the 3' end of said enzymatic RNA molecule. 33. The enzymatic RNA molecule of claim 13, wherein said enzymatic RNA molecule further comprises a 5'-end modification. 34. The enzymatic RNA molecule of claim 14, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted ribose moeity at the 3' end of said enzymatic RNA molecule. 35. The enzymatic RNA molecule of any one of claim 14, wherein said enzymatic RNA molecule further comprises a 3'-3' linked inverted thymidine moeity at the 3' end of said enzymatic RNA molecule. 36. The enzymatic RNA molecule of claim 14, wherein said enzymatic RNA molecule further comprises a 2'-3' linked inverted ribose or thymidine moeity at the 3' end of said enzymatic RNA molecule. 37. The enzymatic RNA molecule of claim 14, wherein said enzymatic RNA molecule further comprises a 5'-end modification. 38. The enzymatic RNA molecule of claim 4, wherein the enzymatic RNA molecule molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 232. 39. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 264. 40. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 266. 41. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 272. 42. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 282. 43. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 300. 44. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 320. 45. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 350. 46. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 374. 47. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 384. 48. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 442. 49. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 522. 50. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 538. 51. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 540. 52. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 93. 53. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 572. 54. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 95. 55. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 614. 56. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 622. 57. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 98. 58. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 2 or 4. 59. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 5 or 6. 60. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 9-11. 61. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 13-16. 62. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 18-20. 63. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 22-25. 64. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 26-29. 65. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 30-33. 66. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 34-37. 67. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 38-41. 68. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 42-45. 69. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 47-50. 70. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 51-54. 71. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 55-58. 72. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 59-62. 73. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 63-66. 74. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 67-70. 75. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 71-74. 76. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 75-78. 77. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 99. 78. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NOS. 100. 79. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 130-133. 80. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 134-137. 81. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves the RNA sequence defined as SEQ ID NO. 138. 82. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 139-141. 83. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 142-145. 84. The enzymatic RNA molecule of claim 6, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 146-148. 85. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 170, 172, 174, or 176. 86. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 178, 180, 182, or 184. 87. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 186, 188, 190, or 192. 88. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 194, 196, 198, or 200. 89. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 202, 204, 206, or 208. 90. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 210, 212, 214, or 216. 91. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 218, 220, 222, or 224. 92. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 226, 228, or 230. 93. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 234, 236, 238, or 240. 94. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NO. 242, 244, 246, or 248. 95. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 250, 252, 254, or 256. 96. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 258, 260, or 262. 97. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 268 or 270. 98. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 274, 276, 278, or 280. 99. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 284, 286, or 288. 100. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 290, 292, 294, or 296. 101. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 298, 302, or 304. 102. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 306, 308, 310, or 312. 103. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 314, 316, or 318. 104. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 322, 324, 326, or 328. 105. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 330, 332, 334, or 336. 106. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 338, 340, 342, or 344. 107. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 346, 348, or 352. 108. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 354, 356, 358, or 360. 109. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 362, 364, 366, or 368. 110. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 370, 372, or 376. 111. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 378, 380, or 382. 112. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 386, 388, 390, or 392. 113. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 394, 396, 398, or 400. 114. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 402, 404, 406, or 408. 115. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 410, 412, 414, or 416. 116. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 418, 420, 422, or 424. 117. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 426, 428, 430, or 432. 118. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 434, 436, 438, or 440. 119. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 444, 446, or 448. 120. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 450, 452, 454, or 456. 121. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 458, 460, 462, or 464. 122. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 466, 468, 470, or 472. 123. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 474, 476, 478, or 480. 124. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 482, 484, 486, or 488. 125. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 490, 492, 494, or 496. 126. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 498, 500, 502, or 504. 127. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 506, 508, 510, or 512. 128. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 514, 516, 518, or 520. 129. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 524, 526, or 528. 130. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 530, 532, 534, or 536. 131. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 540, 542, or 544. 132. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 546, 548, 550, or 552. 133. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 554, 556, 558, or 560. 134. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 562, 564, 566, or 568. 135. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 570, 574, or 576. 136. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 578, 580, 582, or 584. 137. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 586, 588, 590, or 592. 138. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 594, 596, 598, or 600. 139. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 602, 604, 606, or 608. 140. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 610, 612, or 616. 141. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 618, 620, or 624. 142. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 626, 628, 630, or 632. 143. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 634, 638, 640, or 642. 144. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 644, 646, 648, or 670. 145. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 672, 674, 676, or 678. 146. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 680, 682, 684, or 686. 147. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 688, 690, 692, or 694. 148. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 696, 698, 700, or 702. 149. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 704, 706, 708, or 710. 150. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 712, 714, 716, or 718. 151. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 720, 722, 724, or 726. 152. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 728, 730, 732, or 734. 153. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 736, 738, 740, or 742. 154. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 744, 746, 748, or 750. 155. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 752, 754, 756, or 758. 156. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 760, 762, 764, or 766. 157. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 778, 780, 782, or 784. 158. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 786, 788, 790, or 792. 159. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 794, 796, 798, or 800. 160. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 802, 804, 806, or 808. 161. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 810, 812, 814, or 816. 162. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 818, 820, 822, or 824. 163. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 826, 828, 830, or 832. 164. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 834, 836, 838, or 840. 165. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 842, 844, 846, or 848. 166. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 850, 852, 854, or 856. 167. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 858, 860, 862, or 864. 168. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 866, 868, 870, or 872. 169. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 874, 876, 878, or 880. 170. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 882, 884, 886, or 888. 171. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 890, 892, 894, or 896. 172. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 898, 900, 902, or 904. 173. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 906, 908, 910, or 912. 174. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 914, 916, 918, or 920. 175. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 922, 924, 926, or 928. 176. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 930, 932, 934, or 936. 177. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 938, 940, 942, or 944. 178. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 946, 948, 950, or 952. 179. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 954, 956, 958, or 960. 180. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 962, 964, 966, or 968. 181. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 970, 972, 974, or 976. 182. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 978, 980, 982, or 984. 183. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 986, 988, 990, or 992. 184. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 994, 996, 998, or 1000. 185. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1002, 1004, 1006, 1008, or 1010. 186. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1012, 1014, 1016, or 1018. 187. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1020, 1022, 1024, or 1026. 188. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1028, 1030, 1032, or 1034. 189. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1036, 1038, 1040, or 1042. 190. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1042, 1044, 1046, or 1048. 191. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1050, 1052, 1054, or 1056. 192. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1058, 1060, 1062, or 1064. 193. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1066, 1068, 1070, or 1072. 194. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1074, 1076, 1078, or 1080. 195. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1082, 1084, 1086, or 1088. 196. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1090, 1092, 1094, or 1096. 197. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1098, 1100, 1102, or 1104. 198. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1106, 1108, 1110, or 1112. 199. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1114, 1116, 1118, or 1120. 200. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1122, 1124, 1126, or 1128. 201. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1130, 1132, 1134, or 1136. 202. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1138, 1140, 1142, or 1144. 203. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1146, 1148, 1150, or 1152. 204. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1154, 1156, 1158, or 1160. 205. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1162, 1164, 1166, or 1168. 206. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1170, 1172, 1174, or 1176. 207. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1178, 1180, 1182, or 1184. 208. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1186, 1188, 1190, or 1192. 209. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1194, 1196, 1198, or 1200. 210. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1202, 1204, 1206, or 1208. 211. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1210, 1212, 1214, or 1216. 212. The enzymatic RNA molecule of claim 4, wherein said enzymatic RNA molecule specifically cleaves any of the RNA sequences defined as SEQ ID NOS. 1218, 1220, 1222, or 1224. 213. The enzymatic RNA molecule of claim 1, wherein the binding arms of said enzymatic RNA molecule contain sequences perfectly complementary to any of the RNA sequences defined as SEQ. ID. NOS. 1-98, 101-120, or 123-129, wherein said enzymatic RNA molecule is in a hammerhead motif. 214. The enzymatic RNA molecule of claim 1, wherein the the binding arms of said enzymatic RNA molecule contain sequences perfectly complementary to any of the RNA sequences defined as SEQ. ID. NOS. 1-8, wherein said enzymatic RNA molecule is in a hepatitis delta virus motif. 215. The enzymatic RNA molecule of claim 5, wherein the binding arms of said enzymatic RNA molecule contain sequences perfectly complementary to any of the RNA sequences defined as SEQ. ID. NOS. 99, 100, or 130-148, wherein said enzymatic RNA molecule is in a hairpin motif. 216. A mammalian cell including an enzymatic RNA molecule of any one of claims 1, 2, 3, 4, 5, 10, 6 or 7 in vitro. 217. The cell of claim 216, wherein said cell is a human cell. 218. An expression vector including a nucleic acid encoding an enzymatic RNA molecule or multiple enzymatic RNA molecules of claims 1, 2, 3, 4, 5, 10, 6 or 7 in a manner which allows expression of that enzymatic RNA molecule(s) within a mammalian cell. 219. A mammalian cell including an expression vector of claim 218 in vitro. 220. The cell of claim 219, wherein said cell is a human cell.

Details for Patent 5,646,042

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2014-07-08
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2014-07-08
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2014-07-08