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Last Updated: April 6, 2020

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Claims for Patent: 5,593,874

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Summary for Patent: 5,593,874
Title: Enhanced expression in plants
Abstract:This invention provides HSP70 introns that when present in a non-translated leader of a chimeric gene enhance expression in plants.
Inventor(s): Brown; Sherri M. (Chesterfield, MO), Santino; Colleen G. (St. Louis, MO)
Assignee: Monsanto Company (St. Louis, MO)
Application Number:08/333,665
Patent Litigation and PTAB cases: See patent lawsuits and PTAB cases for patent 5,593,874
Patent Claims:1. In a method for the expression of a chimeric plant gene in monocot plants, the improvement which comprises expressing a chimeric plant gene comprising an intron selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 in the non-translated leader 5' of the structural DNA sequence encoding a protein.

2. The method of claim 1 in which the intron is SEQ ID NO:1.

3. The method of claim 1 in which the intron is SEQ ID NO:2.

4. The method of claim 1 in which the intron is SEQ ID NO:3.

5. The method of claim 2 in which the structural DNA sequence encodes a EPSP synthase.

6. The method of claim 2 in which the structural DNA sequence encodes ACC-deaminase.

7. The method of claim 2 in which the structural DNA sequence encodes a GOX protein.

8. The method of claim 2 in which the structural DNA sequence encodes a B.t. crystal toxin protein.

9. The method of claim 3 in which the structural DNA sequence encodes a glgC16 protein.

10. A transgenic monocot plant comprising a DNA construct comprising in sequence:

(a) a promoter that functions in plant cells to cause the production of an RNA sequence;

(b) a non-translated leader comprising an intron sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3;

(c) a structural DNA sequence that causes the production of an RNA sequence that encodes a protein; and

(d) a 3' nontranslated sequence that functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of the RNA sequence; the intron being heterologous with respect to the promoter.

11. The plant of claim 10 in which the intron is the sequence of SEQ ID NO:1.

12. The plant of claim 10 in which the intron is the sequence of SEQ ID NO:2.

13. The plant of claim 10 in which the intron is the sequence of SEQ ID NO:3.

14. The plant of claim 11 in which the plant is maize.

15. The plant of claim 11 in which the plant is wheat.

16. The plant of claim 11 in which the plant is rice.

17. The plant of claim 11 in which the promoter is selected from the group consisting of a CaMV35S promoter and a FMV promoter.

18. The plant of claim 17 wherein the structural DNA sequence encodes for an EPSP synthase.

19. The plant of claim 11 wherein the structural DNA sequence encodes for a CP4 protein.

20. The plant of claim 11 wherein the structural DNA sequence encodes for ACC-deaminase.

21. The plant of claim 11 wherein the structural DNA sequence encodes for a B.t. crystal toxin protein.

22. The plant of claim 17 wherein the structual DNA sequence encodes for glgC16 protein.

23. The plant of claim 11 wherein the structual DNA sequence encodes for a plant viral coat protein.

Details for Patent 5,593,874

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Start Trial Monsanto Company (St. Louis, MO) 2014-01-14 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Start Trial Monsanto Company (St. Louis, MO) 2014-01-14 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Start Trial Monsanto Company (St. Louis, MO) 2014-01-14 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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