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Last Updated: April 19, 2024

Claims for Patent: 5,494,799

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Summary for Patent: 5,494,799

Title:	In vitro assay for detecting cell-mediated immune responses
Abstract:	An in vitro method and kit for the detection of a cell-mediated immune response to a specific antigen, comprising incubating a whole blood sample with the specific antigen and detecting the presence of gamma interferon released by sensitized lymphocytes in the whole blood sample as an indication of a cell-mediated immune response to the specific antigen.
Inventor(s):	Wood; Paul R. (Lower Templestowe, AU), Corner; Leigh A. (Romsey, AU)
Assignee:	Commonwealth Scientific & Industrial Research Organisation (Campbell, AU)
Application Number:	08/230,373
Patent Claims:	1. A diagnostic kit for the detection of a cell-mediated immune response to a specific antigen in a human or animal, comprising: a. a source of the specific antigen; b. means for incubating said specific antigen with a whole blood sample from a human or animal; and c. means for detecting the presence of gamma interferon (.gamma.IFN) released by sensitized lymphocytes in the whole blood sample to indicate a cell-mediated immune response to the specific antigen. 2. A kit according to claim 1, wherein said means for detecting the presence of gamma interferon comprises means for performing an immunoassay for gamma interferon. 3. A kit according to claim 2, wherein said immunoassay means comprises an enzyme-linked immunosorbent assay (ELISA) or a radio-immunoassay (RIA) for gamma interferon. 4. A kit according to claim 1 wherein said specific antigen is an antigen of Mycobacterium bovis, Mycobacterium paratuberculosis, or Mycobacterium tuberculosis. 5. A kit according to claim 1, for the detection of M. bovis infections in cattle, wherein specific antigen is the M. bovis antigen, tuberculin purified protein derivative (PPD). 6. A kit according to claim, wherein said specific antigen is an antigen of Mycobacterium bovis, Mycobacterium paratuberculosis, or Mycobacterium tuberculosis. 7. A kit according to claim 3, wherein said specific antigen is an antigen of Mycobacterium bovis, Mycobacterium paratuberculosis, or Mycobacterium tuberculosis. 8. A diagnostic kit for the detection of a cell-mediated immune response to a specific antigen in an human or animal which comprises: a) a source of specific antigens selected from the group consisting of Mycobacterium bovis, Mycobacterium paratuberculosis, and Mycobacterium tuberculosis; b) means for incubating said specific antigen with a whole blood sample from human or animal; and d) means for detecting the presence of a gamma interferon released by sensit lymphocytes in the whole blood sample to indicated a cell-mediated immune response to the specific antigen. 9. A diagnostic kit for the detection of a cell-mediated immune response to a specific antigen in cattle which comprises: a) a source of specific antigen which is the M. bovis antigen, tuberculin purified protein derivative (PPD); b) means for incubating said specific antigen with a whole blood sample from cattle; and c) means for detecting the presence of gamma interferon released by sensitized lymphocytes in the whole blood sample to indicate a cell-mediated immune response to the specific antigen.

Details for Patent 5,494,799

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Par Pharmaceutical Companies, Inc.	APLISOL	tuberculin, purified protein derivative	Injection	103782	04/20/1998	⤷ Try a Trial	2006-03-06
Sanofi Pasteur Limited	TUBERSOL	tuberculin, purified protein derivative	Injection	103941	02/24/2000	⤷ Try a Trial	2006-03-06
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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