Claims for Patent: 5,422,261
✉ Email this page to a colleague
Summary for Patent: 5,422,261
Title: | Composition containing collagenase and chymopapain for hydrolyzing connective tissue to isolate cells |
Abstract: | Proteolytic enzyme compositions and processes for digesting connective tissue are disclosed. The enzyme compositions include collagenase, which is essentially free of toxins and non-collagen specific components, and chymopapain, which is essentially free of toxins. The enzyme compositions are used for dissociating microvessel cells from connective tissue. Recovered microvessel cells are incorporated into artificial vessel grafts. The enzyme compositions preferably contain an aqueous mixture of collagenase having an activity of about 43 nkat/ml to about 51 nkat/ml, and chymopapain having an activity of about 0.22 nkat/ml to about 0.44 nkat/ml. |
Inventor(s): | Lee; Catherine T. (Laguna Hills, CA), Hornacek; Cynthia (Trabuco cyn, CA), Dinh; Tan T. (Garden Grove, CA) |
Assignee: | Baxter International Inc. (Deerfield, IL) |
Application Number: | 08/049,015 |
Patent Claims: | 1. An enzyme composition useful for hydrolyzing connective tissue, said composition comprising:
an aqueous mixture of collagenase having an activity of about 43 nkat/ml to about 51 nkat/ml, and chymopapain having an activity of about 0.22 nkat/ml to about 0.44 nkat/ml, in a physiologically compatible electrolyte solution buffered to a pH of about 7.0 to 7.4, wherein the activity of collagenase in nkat/ml is defined as the number of nanomoles of furylacryloyl Leu-Gly-Pro-Ala synthetic substrate (FALGPA) hydrolyzed per second by 1 ml of collagenase solution and the activity of chymopapain in nkat/ml is defined as the number of nanomoles of Benzoyl-L-arginine-p-nitroanilide synthetic substrate (BAPNA) hydrolyzed per second by 1 ml of chymopapain solution, said collagenase being essentially free of toxins and non-collagen active components and said chymopapain being essentially free of toxins. 2. The enzyme composition of claim 1 wherein said electrolyte solution is a phosphate buffered saline solution. 3. The enzyme composition of claim 1 wherein said electrolyte solution is an aqueous solution of sodium, potassium, magnesium, chloride, acetate, and gluconate ions buffered to a pH of 7.4, and having an osmolarity of 294 mOsmol/L. 4. The enzyme composition of claim 1 further comprising about 0.4 wt % human serum albumin. |
Details for Patent 5,422,261
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Discure Medical, Llc | CHYMODIACTIN | chymopapain | For Injection | 018663 | 11/10/1982 | ⤷ Try a Trial | 2039-03-29 |
Discure Medical, Llc | CHYMODIACTIN | chymopapain | For Injection | 018663 | 08/21/1984 | ⤷ Try a Trial | 2039-03-29 |
Smith & Nephew, Inc. | SANTYL | collagenase | Ointment | 101995 | 06/04/1965 | ⤷ Try a Trial | 2039-03-29 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
Make Better Decisions: Try a trial or see plans & pricing
Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.