You’re using a public version of DrugPatentWatch with 5 free searches available | Register to unlock more free searches. CREATE FREE ACCOUNT

Last Updated: March 28, 2024

Claims for Patent: 5,320,962


✉ Email this page to a colleague

« Back to Dashboard


Summary for Patent: 5,320,962
Title: DNA encoding the human A1 adenosine receptor
Abstract:Genomic and complimentary DNA encoding the human A.sub.1 adenosine receptor are disclosed, along with vectors and host cells containing the same, oligonucleotides and antisense oligonucleotides directed to the same (particularly antisense oligonucleotides directed to an intron/exon junction), and methods of using the foregoing.
Inventor(s): Stiles; Gary L. (Chapel Hill, NC), Ren; Hongzu (Durham, NC), Olah; Mark (Durham, NC)
Assignee: Duke University (Durham, NC)
Application Number:07/918,314
Patent Claims:1. Isolated DNA encoding an A.sub.1 adenosine receptor selected from the group consisting of:

(a) isolated human genomic DNA which encodes the human A.sub.1 adenosine receptor given herein as SEQ ID NO:6 and which contains the DNA sequences given herein as SEQ ID NO:1 and SEQ ID NO:3;

(b) isolated human genomic DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60.degree. C., and which encodes a human A.sub.1 adenosine receptor; and

(c) isolated DNA differing from the isolated DNAs of (a) and (b) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes an A.sub.1 adenosine receptor encoded by isolated DNA of (a) or (b) above.

2. A recombinant DNA comprising vector DNA and a DNA according to claim 1.

3. A recombinant DNA according to claim 2, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

4. A recombinant DNA according to claim 2, wherein said vector DNA comprises a baculovirus vector.

5. A host cell containing a recombinant DNA of claim 2 and capable of expressing the encoded protein.

6. A host cell according to claim 5, wherein said host cell is a mammalian cell.

7. A host cell according to claim 5, wherein said host cell is selected from the group consisting of baby hamster kidney cells, mouse cells, human embryo cells, and chinese hamster ovary cells.

8. A host cell according to claim 5, wherein said host cell is an insect cell.

9. Isolated DNA encoding an A.sub.1 adenosine receptor selected from the group consisting of:

(a) isolated DNA which encodes a human A.sub.1 adenosine receptor comprising DNA having the sequence given herein as SEQ ID NO:5;

(b) isolated DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60.degree. C., and which encodes a human A.sub.1 adenosine receptor; and

(c) isolated DNA differing from the isolated DNAs of (a) and (b) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes a human A.sub.1 adenosine receptor encoded by isolated DNA of (a) or (b) above.

10. A recombinant DNA comprising vector DNA and a DNA according to claim 9.

11. A recombinant DNA according to claim 10, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

12. A recombinant DNA according to claim 10, wherein said vector DNA comprises a baculovirus vector.

13. A host cell containing a recombinant DNA of claim 10 and capable of expressing the encoded protein.

14. A host cell according to claim 13, wherein said host cell is a mammalian cell.

15. A host cell according to claim 13, wherein said host cell is selected from the group consisting of baby hamster kidney cells, mouse cells, human embryo cells, and chinese hamster ovary cells.

16. A host cell according to claim 13, wherein said host cell is an insect cell.

17. An oligonucleotide capable of binding to an intron of the A.sub.1 adenosine receptor gene, said oligonucleotide selected from the group consisting of:

(a) (i) isolated DNA comprising the first intron of the human genomic DNA encoding the human A.sub.1 adenosine receptor according to SEQ ID NO:6, (ii) isolated DNA comprising the second intron of the human genomic DNA encoding the human A.sub.1 adenosine receptor according to SEQ ID NO:6, (iii) isolated DNA comprising the third intron of the human genomic DNA encoding the human A.sub.1 adenosine receptor according to SEQ ID NO:6, (iv) isolated DNA comprising the fourth intron of the human genomic DNA encoding the human A.sub.1 adenosine receptor according to SEQ ID NO:6, and (v) isolated DNA comprising the fifth intron of the human genomic DNA encoding the human A.sub.1 adenosine receptor according to SEQ ID NO:6; and

(b) isolated DNA which hybridizes to the isolated DNAs of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60.degree. C.

18. An oligonucleotide according to claim 17 labelled with a detectable group.

19. A recombinant DNA comprising vector DNA and an oligonucleotide according to claim 17.

20. A recombinant DNA according to claim 19, wherein said DNA is capable of exchanging said oligonucleotide with a homologous nucleotide in a suitable cell by homologous recombination.

21. Isolated human genomic DNA which encodes the human A.sub.1 adenosine receptor given herein as SEQ ID NO:6 and which contains the DNA sequences given herein as SEQ ID NO:1 and SEQ ID NO;3.

22. A recombinant DNA comprising vector DNA and DNA according to claim 21.

23. A recombinant DNA according to claim 22, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

24. A recombinant DNA according to claim 22, wherein said vector DNA comprises a baculovirus vector.

25. A host cell containing a recombinant DNA of claim 22 and capable of expressing the encoded protein.

26. Isolated DNA which encodes a human A.sub.1 adenosine receptor having the sequence given herein as SEQ ID NO:5.

27. A recombinant DNA comprising vector DNA and a DNA according to claim 26.

28. A recombinant DNA according to claim 27, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

29. A recombinant DNA according to claim 27, wherein said vector DNA comprises a baculovirus vector.

30. A host cell containing a recombinant DNA of claim 27 and capable of expressing the encoded protein.

Details for Patent 5,320,962

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2039-02-26
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

Make Better Decisions: Try a trial or see plans & pricing

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.