Claims for Patent: 5,316,942

Summary for Patent: 5,316,942

Title:	Process for the production of low-cost soluble high-molecular weight collagen
Abstract:	The invention includes a method that produces a low cost aqueous solution of high molecular weight solubilized collagen by the steps of: (a) providing an aqueous ground slurry of insoluble collagen; (b) adjusting the water or solid content of the wet ground slurry whereby the insoluble collagen is at a concentration that promotes substantially maximum solubilized collagen concentration and molecular weight in a final product; (c) adjusting the pH of the slurry from Step b to obtain activity for a proteolytic enzyme added in Step d; (d) adding the proteolytic enzyme to the pH adjusted slurry and reacting at a temperature, T, and for a time, t, effective for forming high molecualr weight solubilized collagen from the insoluble collagen particles; (e) controlling the reaction conditions for obtaining a high concentration of soluble collagen and a high molecular weight of the solubilized collagen by simultaneously measuring the concentration of solubilized collagen and the molecular weight of the solubilized collagen, whereby the reaction is complete when the molecular weight and the concentration are substantially maximized; and (f) withdrawing the aqueous solution of high molecular weight solubilized collagen as product. Proteolytic enzyme recycle steps are disclosed that can be used to further reduce costs.
Inventor(s):	Fink; David J. (Shaker Heights, OH)
Assignee:	Battelle Memorial Institute (Columbus, OH)
Application Number:	08/078,932
Patent Claims:	1. A method for producing an aqueous solution of high molecular weight solubilized collagen comprising: a. providing an aqueous ground slurry of insoluble collagen; b. adjusting the water or solid content of said wet ground slurry whereby said insoluble collagen is at a concentration that promotes substantially maximum solubilized collagen concentration and molecular weight in a final product; c. adjusting the pH of said slurry from Step b to obtain activity for a proteolytic enzyme added in Step d; d. adding said proteolytic enzyme to said pH adjusted slurry and reacting at a temperature, T, and for a time, t, effective for forming a solution of high molecular weight solubilized collagen from said insoluble collagen particles; e. controlling said reaction to obtain a high concentration of soluble collagen and a high molecular weight of said solubilized collagen by simultaneously measuring the concentration of solubilized collagen and the molecular weight of said solubilized collagen, whereby said reaction is complete when said molecular weight and said concentration are substantially maximized; f. adding additional water and insoluble collagen to said solution containing high molecular weight solubilized collagen in Step d and mixing; g. separating at least some of the solution containing high molecular weight solubilized collagen from the insoluble collagen and returning the insoluble collagen to Step b, whereby at least a portion of the proteolytic enzyme is recycled, and the separated solution containing high molecular weight solubilized collagen is withdrawn as product. 2. The method of claim 1, further comprising repeating Steps f and g one or more times. 3. The method of claim 1, comprising stopping said reaction by (1) adjusting said pH to that where said proteolytic enzyme is substantially inactive; and/or (2) reducing the temperature to that where said proteolytic enzyme is substantially inactive. 4. The method of claim 1, comprising controlling said reaction by measuring solution viscosity at two different shear rates whereby said reaction is complete when a ratio of, (viscosity at low shear)/(viscosity at high shear) is substantially maximized. 5. The method of claim 1, further comprising said viscosity ratio is at least 75% of maximum. 6. The method of claim 1, further comprising in Step b, adjusting said liquid or solids content of said wet ground slurry so that said solids are adjusted to a concentration of about 0.1 to about 1.0 wt %. 7. The method of claim 1, whereby said temperature, T, comprises about 5.degree. C. to about 35.degree. C. 8. The method of claim 1, whereby said temperature, T, comprises about 15.degree. C. to about 30.degree. C. 9. The method of claim 1, whereby, in Step b, said solids concentration is between about 0.3 to 0.35 wt %; and in Step e. controlling said reaction at a temperature of about 20.degree. to about 30.degree. C., and for time of 24 to 48 hours. 10. The method of claim 1, further comprising: selecting the proteolytic enzyme from the group consisting of porcine mucosal pepsin, bromelain, chymopapain, chymotrypsin, collagenase, ficin, papain, peptidase, proteinase A, proteinase K, trypsin, and combinations of such enzymes. 11. The method of claim 10, further comprising: adjusting the pH to about 1.5-3.0 and the temperature to between about 26.degree. to about 28.degree. C., when porcine mucosal pepsin is selected. 12. The method of claim 1, further comprising: whereby at least 80 wt % of said insoluble collagen is converted to soluble collagen and the number average molecular weight is above 300,000 daltons. 13. The method of claim 12, further comprising: whereby at least 80 wt % of said insoluble collagen is converted to soluble collagen and the number average molecular weight is above 600,000 daltons. 14. The method of claim 1, further comprising: whereby at least 90 wt % of said insoluble collagen is converted to soluble collagen and the number average molecular weight is above 1,000,000 daltons.

Details for Patent 5,316,942

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Discure Medical, Llc	CHYMODIACTIN	chymopapain	For Injection	018663	11/10/1982		2039-03-29
Discure Medical, Llc	CHYMODIACTIN	chymopapain	For Injection	018663	08/21/1984		2039-03-29
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2039-03-29