Claims for Patent: 5,314,822

Summary for Patent: 5,314,822

Title:	Method of clonal growth of Streptococcus pneumoniae
Abstract:	A culture medium containing no complex animal components is useful for production of bacteria and inoculum development from which pharmaceutically important products are to be isolated.
Inventor(s):	Bailey; Fred J. (Hatfield, PA), Herber; Wayne K. (Center Valley, PA)
Assignee:	Merck & Co., Inc. (Rahway, NJ)
Application Number:	07/961,587
Patent Claims:	1. A method for clonal growth of Streptococcus pneumoniae from the single colony stage in a medium containing no bovine derived, ovine derived or crude blood derived products which comprises: (a) preparing an agar plate of said medium streaked with said Streptococcus pneumoniae, and growing said Streptococcus pneumoniae, wherein said agar plate comprises agar, Yeast Extract, Soy Peptone, salts to maintain buffering and osmolarity, Vitamin K if known to be required for the particular strain being isolated, Hemin, Sodium Bicarbonate, L-cysteine HCl, Glucose, a buffer to maintain the pH at between about pH 7.3-7.8, but no bovine derived, ovine derived or crude blood-derived products; and (b) selecting a single colony for expansion by growing in a culture of the same medium used in step (a) absent the agar, and optionally storing the culture as a frozen glycerol stock. 2. The method of claim 1 wherein the medium in step (a) is an SYG agar plate streaked with said Streptococcus pneumoniae, wherein the SYG agar plate contains, on a per liter basis, about 20 g of agar and: wherein said salt solution contains CaCl.sub.2 (0.2 g anhydrous), MgSO4 (0.2 g anhydrous), K.sub.2 HPO.sub.4 (1.0 g), KH.sub.2 PO.sub.4 (1.0 g), NaHCO.sub.3 (10 g), NaCl (2 g).

Details for Patent 5,314,822

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	PANHEMATIN	hemin for injection	For Injection	101246	07/20/1983		2040-01-28