Claims for Patent: 5,273,888

Summary for Patent: 5,273,888

Title:	Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
Abstract:	A chemical test kit for determining the presence of occult blood in a stool specimen is disclosed which includes an improved monitor system for verifying the effectiveness of the chemicals used in the test. A method of testing for occult blood is also disclosed which method includes verifying the effectiveness of the chemicals utilized in the test.
Inventor(s):	Guadagno; Philip A. (Vidor, TX)
Assignee:	Helena Laboratories Corporation (Beaumont, TX)
Application Number:	07/187,674
Patent Claims:	1. In a test kit for determining the presence of blood in a specimen, the test kit including a sample test area having a chromogen associated therewith; and said test kit further including an oxygen generator, said oxygen generator for liberating oxygen in the presence of a catalyst exhibiting peroxidase-like activity, the liberated oxygen for causing the chromogen to undergo a chromogenic reaction; the improvement comprising: a first reagent test area; a monitor for testing the effectiveness of the chromogen and for verifying that the chromogen has retained the ability to undergo a chromogenic reaction, said monitor having sufficient oxidation potential to effect oxidation of the chromogen when the monitor and chromogen are in liquid contact, said monitor being sufficiently inert relative to the chromogen such that the chromogen does not undergo a chromogenic reaction when the monitor and chromogen are in substantially non-liquid contact, said monitor being hemoglobin-free and hemin-free; and a second reagent test area, spaced apart from said first reagent test area, said second reagent test area including a chromogen and a vegetable peroxidase, said second reagent test area, said second reagent test area for determining the effectiveness of a peroxidase inactivating solution. 2. The test kit of claim 1, wherein said chromogen is guaiac. 3. The test kit of claim 1, wherein said oxygen generator includes a peroxide. 4. The test kit of claim 1, wherein said monitor is selected from the group consisting of hypochlorites, peroxidases, and metal salts of lead, copper, calcium, iron and nickel. 5. The test kit of claim 1, wherein said sample test area is formed as a slide means. 6. The test kit of claim 1, wherein said monitor has an oxidation potential sufficient to catalyze a chromogenic reaction in said chromogen when said oxygen donor, said chromogen and said monitor are in liquid contact by liberation of oxygen from said oxygen donor. 7. The test kit of claim 1 wherein said oxygen generator is hydrogen peroxide. 8. The test kit of claim 1 wherein said oxygen generator is in solution. 9. The test kit of claim 2 wherein said first and second test areas are formed as part of a slide. 10. The test kit of claim 3 wherein said monitor is selected from the group consisting of hypochlorites, peroxidases, and metal salts of lead, copper, calcium, iron and nickel. 11. The test kit of claim 1 wherein the monitor and chromogen are formed on a slide.

Details for Patent 5,273,888

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	PANHEMATIN	hemin for injection	For Injection	101246	07/20/1983		2010-12-28