Claims for Patent: 5,221,738

Summary for Patent: 5,221,738

Title:	cDNA and genomic DNA encoding the amino acid sequence of human .alpha..sub.2 -plasmin inhibition
Abstract:	cDNA encoding a human .alpha..sub.2 -plasmin inhibitor precursor protein represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; an amino acid sequence of a human .alpha..sub.2 -plasmin inhibitor precursor represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; and genomic DNA encoding a human .alpha..sub.2 -plasmin inhibitor protein which is composed of exons II, III, IV, V, VI, VII, VIII, IX and X in FIG. 4 of the accompanying drawings, said exons being bonded to one another via introns.
Inventor(s):	Sumi; Yoshihiko (Hino, JP), Ichikawa; Yataro (Tokorozawa, JP), Murakami; Masami (Tokorozawa, JP), Aoki; Nobuo (Tokyo, JP)
Assignee:	Teijin Limited (Osaka, JP)
Application Number:	07/803,896
Patent Claims:	1. cDNA encoding a human .alpha..sub.2 -plasmin inhibitor precursor protein which consists of the following amino acid sequence: ##STR2## 2. The cDNA of claim 1 which consists of the following base sequence: ##STR3## 3. cDNA encoding a leader amino acid sequence which consists of the following amino acid sequence: ##STR4## 4. cDNA of claim 3 which consists of the following base sequence: ##STR5## 5. Genomic DNA encoding a human .alpha..sub.2 -plasmin inhibitor protein consisting of exons II, III, IV, V, VI, VII, VIII, IX and X having base sequences as shown below, said exons being connected to one another via introns which have base sequences or sizes as shown below, a first portion of said genomic DNA consisting of from exons II to VII and being contained in the base sequence of pUC19 plasmid, pPI62 bearing ATCC Designation No. 75142, and a second portion of said genomic DNA consisting of from exons VII to X and being contained in the base sequence of charon 4A phage, lambda PI2 bearing ATCC Designation No. 75143: ##STR6## 6. The genomic DNA of claim 5 consisting of the following base sequence, wherein exon I having the following base sequence is linked upstream of exon II via intron I having a size of about 8kb, the first portion of said genomic DNA consisting of from exons I to VII and being contained in the base sequence of pUC19 plasmid, pPI62 bearing ATCC Designation No. 75142, and the second portion of said genomic DNA consisting of from exons VII to X and being contained in the base sequence of charon 4A phage, lambda PI2 bearing ATCC Designation No. 75143: ##STR7## 7. Genomic DNA encoding a leader amino acid sequence which consists of exon II, intron 2, exon III, intron 3 and exon IV having the following base sequences, said genomic DNA being contained in the base sequence of pUC19 plasmid, pPI62 bearing ATCC Designation No. 75142: ##STR8##

Details for Patent 5,221,738

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2010-06-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2010-06-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2010-06-22