Claims for Patent: 5,109,120

Summary for Patent: 5,109,120

Title:	Reduction of chemically modified proteins
Abstract:	The invention provides chemically modified proteins having a group of the formula: ##STR1## wherein R represents a hydrogen atom or a lower alkyl, m represents an optional positive integer and n represents an integer 1 to 4, the group being bonded to at least one primary amino group of the protein, and a method of producing the same. The chemically modified proteins according to the invention can be produced by reacting a protein with an imidoester of the formula: ##STR2## wherein R, n and m are as defined above, R\' represents a group constituting an imidoester with an adjacent imidoyl group. The chemically modified proteins according to the invention are useful as drugs, among others.
Inventor(s):	Ueno; Hayao (Suita, JP), Fujino; Masahiko (Takarazuka, JP)
Assignee:	Takeda Chemical Industries, Ltd. (Osaka, JP)
Application Number:	07/364,707
Patent Claims:	1. A method of producing a chemically modified protein having imidoyl groups whose number is from one to the number of primary amino groups of the protein of the formula: ##STR8## wherein R represents a hydrogen atom or an alkyl group having 1 to 18 carbon atoms, m represents a positive integer from 7 to 150 and n represents an integer 1 to 4, each group being bonded to one primary amino group of the protein, wherein the molecular weight of the group of the formula (I) is from 350 to 7,000, which comprises reacting a protein with an imidoester of the formula: ##STR9## wherein R, m and n are as previously defined, R' represents a group of the formula: --OR", wherein R" represents a substituted or unsubstituted alkyl groups having 1 to 8 carbon atoms, wherein the substituents are one or two chloro or fluora, or a substituted or unsubstituted aralkyl group selected from the group consisting of benzyl, phenethye and phenylpropyl, wherein the substituents are one or two alkyl groups having 1 to 3 carbon atoms which forms an imidoester with the imidoyl group (I), or with an imidoester salt of the formula: ##STR10## wherein R, R', m and n are as previously defined, x is an integer of 1-3 and HxX is an acid selected from the group consisting of HCl, H2SO4, CF3COOH, HClO.sub.4, CH3COOH, CCl3COOH, and H.sub.3 PO.sub.4. 2. The method according to claim 1, wherein the protein is (1) a cytokine selected from the groups consisting of interferon-.alpha., interferon-.beta., interferon-.gamma. and interleukin-2, (2) a hormone selected from the group consisting of growth hormones and insulin, (3) an enzyme selected from the group consisting of urokinase, superoxide dismutase and asparaginase, or (4) a protein selected from the group consisting of immunoglobulins, islet-activating proteins, inhibitor proteins and neocarzinostatin. 3. The method according to claim 2, wherein the protein is an interferon selected from the group consisting of interferon-.alpha., interferon-.beta. and interferon-.gamma.. 4. The method according to claim 3, wherein the protein is interferon-.gamma. modified with an imidoester of polyethylene glycol monomethyl ether. 5. The method according to claim 2, wherein the protein is interleukin-2 modified with an imidoester of poyethylene glycol monomethyl ether. 6. The method according to claim 2, wherein the protein is superoxide dismutase modified with an imidoester of polyethylene glycol monomethyl ether. 7. The method according to claim 1, wherein the reaction is conducted at pH 7 to 9, and 0.degree. C. to 40.degree. C. for 0.5 to 72 hours. 8. The method according to claim 1, wherein R" is benzyl, phenethyl or phenylpropyl. 9. The method according to claim 1, wherein R is methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl, n-pentyl, iso-pentyl, n-hexyl, iso-hexyl, n-heptyl, iso-heptyl, n-octyl, iso-octyl, n-nonyl, iso-nonyl, n-decyl, iso-decyl, n-undecyl, n-dodecyl, n-tridecyl, n-tetradecyl, n-pentadecyl, n-hexadecyl, n-heptadecyl or n-octdecyl. 10. The method according to claim 1, wherein n is 2 or 3. 11. The method according to claim 1, wherein the primary amino group is N-terminal .alpha.-amino group or .xi.-amino group of lysine residue in the protein. 12. The method according to claim 6, which has the group of formula (I) bonded to 5 to 80% of .xi.-amino groups of lysine residue in the protein. 13. The method according to claim 1, wherein the group of formula (I) has molecular weight corresponding to 1 to 10% of the molecular weight of the modified protein.

Details for Patent 5,109,120

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Microbix Biosystems Inc.	KINLYTIC	urokinase	For Injection	021846	01/16/1978		2009-04-28
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2009-04-28