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Last Updated: March 18, 2024

Claims for Patent: 5,102,796


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Summary for Patent: 5,102,796
Title: Plant structural gene expression
Abstract:The invention provides genetically modified plant cells having a plant structural gene introduced and expressed therein under the control of a T-DNA promoter. In particular, the invention provides genetically modified plant cells having the phaseolin gene introduced and expressed therein under the control of a T-DNA promoter. Also provided are novel strains of bacteria containing and replicating T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter. Further, the invention provides plasmids having the ability to replicate in E. coli and comprising T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter.
Inventor(s): Hall; Timothy C. (College Station, TX), Kemp; John D. (Las Cruces, NM), Slightom; Jerry L. (Kalamazoo, IL), Sutton; Dennis W. (Las Cruces, NM)
Assignee: Lubrizol Genetics, Inc. (Wickliffe, OH)
Application Number:07/144,775
Patent Claims:1. A DNA vector comprising T-DNA having a plant structural gene inserted therein under control of a T-DNA promoter.

2. A DNA vector according to claim 1 wherein the plant structural gene comprises an intron.

3. A DNA vector according to claim 1 wherein the plant structural gene is under control of a promoter selected, from the group of T-DNA genes consisting of tmr, tml, tms, nopaline synthase, octopine synthase, or the 1.6 transcript.

4. A DNA vector according to claim 1 wherein the plant structural gene is modified.

5. A DNA vector according to claim 4 wherein the plant structural gene modification comprises removal of an intron.

6. A DNA vector according to claim 4 wherein the plant structural gene comprises cDNA.

7. A DNA vector according to claim 4 wherein the plant structural gene modification comprises a DNA segment insertion.

8. A DNA vector according to claim 4 wherein the plant structural gene modification comprises a DNA segment deletion.

9. A DNA vector according to claim 1 wherein the T-DNA is modified.

10. A DNA vector according to claim 9 wherein the T-DNA modification comprises a mutation in tms.

11. A DNA vector according to claim 9 wherein the T-DNA modification comprises a mutation in tmr.

12. A DNA vector according to claim 9 wherein the T-DNA modification comprises a deletion in T-DNA.

13. A DNA vector according to claim 4 wherein the T-DNA promoter includes part of the coding region of the T-DNA gene normally controlled by said promoter.

14. A DNA vector according to claim 13 wherein the inserted plant structural gene comprises an intron.

15. A DNA vector according to claim 13 wherein the plant structural gene codes for phaseolin.

16. A DNA vector according to claim 15 wherein the plant structural gene coding for phaseolin is inserted under control of a promoter selected from the group of T-DNA genes consisting of tmr, tml, tms, nopaline synthase, octopine synthase, or the 1.6 transcript.

17. A DNA vector according to claim 1 selected from the group consisting of pKS4, p3.8, pcDNA31, or pPVL134.

18. A Bacterial strain containing and replicating a plasmid comprising T-DNA having a plant structural gene inserted therein under control of a T-DNA promoter.

19. The bacterial strain of claim 18 comprising a TIP plasmid modified to contain within it said T-DNA having a plant structural gene inserted therein under control of a T-DNA promoter.

20. The bacterial strain of claim 19 comprising Agrobacterium tumefaciens or Agrobacterium rhizogenes.

21. The bacterial strain of claim 20 wherein the TIP plasmid is p15955-12A.

22. The bacterial strain of claim 20 wherein the TIP plasmid comprises a modification that inactivates the tms gene.

23. The bacterial strain of claim 20 wherein the TIP plasmid comprises a modification that inactivates the tmr gene.

24. The bacterial strain of claim 22 wherein the TIP plasmid is pA66-12A.

25. The bacterial strain of claim 18 comprises a TIP plasmid and a sub-TIP plasmid, the sub-TIP plasmid having a plant structural gene inserted therein under control of a T-DNA promoter.

26. A bacterial strain according to claim 18 wherein the plasmid comprising T-DNA and having a plant structural gene inserted therein under control of a T-DNA promoter is selected from the group: pKS4, p3.8, pcDNA 31 or pPVL 134.

27. A bacterial strain according to claim 18 selected from the group A. tumefaciensp15955-12A, E. coli c600/pKS4, E. coli HB101/p3.8, E. coli HB101/pcDNA31, or E. coli HB101/pPVL134.

28. A DNA molecule comprising in linear sequence:

(a) a first DNA segment comprising plant genomic DNA;

(b) a second DNA segment comprising T-DNA;

(c) a third DNA segment comprising a plant structural gene and a T-DNA promoter in such position and orientation with respect to each other that said plant structural gene is expressible in a plant cell under the control of said T-DNA promoter;

(d) a fourth DNA segment comprising T-DNA; and

(e) a fifth DNA segment comprising plant genomic DNA.

29. A DNA molecule according to claim 28 that is a plant chromosome.

30. A DNA molecule according to claim 28 wherein said T-DNA promoter is selected from the group consisting of tmr, tml, tms, nopaline synthase, octopine synthase, and the 1.6 transcript.

31. A DNA molecule according claim 28 wherein said plant structural gene containes at least one intron.

32. A DNA molecule according to claim 28 wherein said plant structural gene comprises cDNA.

Details for Patent 5,102,796

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2009-04-07
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2009-04-07
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2009-04-07
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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