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Last Updated: April 18, 2024

Claims for Patent: 5,055,415

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Summary for Patent: 5,055,415

Title:	Method for immunoassay procedure for the detection of antigen by reacting with antibody which increases its electrical charge and electrophoretic mobility
Abstract:	A method for immunoassay for an antigen involves the following steps: reacting a sample possibly containing an antigen to be determined, a definite amount of a conjugate of the antigen and a charged substance and a definite amount of labeled antibody specific for antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached antibody specific for the charged substance, so that when the reaction products contact the carrier any reaction product of labeled antibody and antigen passes through the carrier and any reaction product of the labeled antibody and conjugate binds to the carrier attached antibody specific for the charged substance; determining the amount of labeled antibody thereby bound to the carrier and relating the determined amount of labeled antibody to the amount of antigen in the sample.
Inventor(s):	Imai; Kyoko (Katsuta, JP), Nomura; Yasushi (Mito, JP)
Assignee:	Hitachi, Ltd. (Tokyo, JP)
Application Number:	07/177,347
Patent Claims:	1. A method for immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of labeled antibody specific for antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached antibody specific for the charged substance, whereby when said reaction products contact said carrier 1) any reaction product of labeled antibody and antigen passes through said carrier, and 2) any reaction product of labeled antibody and conjugate binds to the carrier attached antibody specific for the charged substance; determining the amount of labeled antibody thereby bound to the carrier; relating the determined amount of labeled antibody to the amount of antigen in the sample. 2. A method for immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of a non-labeled first antibody specific for the antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the charged substance, whereby when said reaction products contact said carrier 1) any reaction product of first antibody and antigen passes through said carrier, and 2) any reaction product of first antibody and conjugate becomes bound to the carrier attached second antibody specific for the charged substance; reacting the first antibody thereby bound to the carrier with a labeled third antibody specific for the non-labeled first antibody; determining the amount of labeled third antibody thereby bound to the carrier; relating the determined amount of labeled third antibody to the amount of antigen in the sample. 3. A method of immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a labeled charged substance, and 3) a definite amount of a first antibody specific for antigen; allowing any reaction products or unreacted conjugate to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the first antibody, whereby when said reaction products or unreacted conjugate contact said carrier 1) any unreacted conjugate passes through said carrier, and 2) any reaction product of first antibody with antigen or conjugate binds to the carrier attached second antibody; determining the amount of label in the conjugate thereby bound to the carrier; relating the determined amount of label to the amount of antigen in the sample. 4. A method of immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of a first antibody specific for the antigen; allowing any reaction products or unreacted conjugate to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the first antibody, whereby when said reaction products or unreacted conjugate contact said carrier 1) any unreacted conjugate passes through said carrier, and 2) any reaction product of first antibody with antigen or conjugate binds to the carrier attached second antibody; reacting the conjugate thereby bound to the carrier with a labeled third antibody specific for the charged substance; determining the amount of labeled third antibody thereby bound to the carrier; relating the determined amount of labeled third antibody to the amount of antigen in the sample. 5. The method for immunossay according to any one of claimes 1 to 4 wherein the antigen to be determined is apparently electrically neutral or has no or slight electric chage so that the antigen cannot migrate or can slightly migrate by electrophoresis. 6. The method for immunossay according to any one of claims 1 to 4 wherein the charged substance is a protein or a lipid. 7. The method for immunossay according to claim 6 wherein the protein is bovine serum albumin, rabbit serum albumin, human serum albumin, or globulin. 8. The method for immunossay according to claim 6 wherein the lipid is dicetyl phosphoric acid.

Details for Patent 5,055,415

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Grifols Therapeutics Llc	ALBUKED, PLASBUMIN-20, PLASBUMIN-25, PLASBUMIN-5	albumin (human)	For Injection	101138	10/21/1942	⤷ Try a Trial	2008-10-08
Baxalta Us Inc.	BUMINATE, FLEXBUMIN	albumin (human)	Injection	101452	03/03/1954	⤷ Try a Trial	2008-10-08
Csl Behring Ag	ALBURX	albumin (human)	Injection	102366	07/23/1976	⤷ Try a Trial	2008-10-08
Grifols Biologicals Llc	ALBUTEIN	albumin (human)	Injection	102478	08/15/1978	⤷ Try a Trial	2008-10-08
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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