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Last Updated: March 29, 2024

Claims for Patent: 5,034,428


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Summary for Patent: 5,034,428
Title: Immobilized biomolecules and method of making same
Abstract:A method of biomolecule immobilization is described in which the biomolecule itself or, alternatively, a monomer-conjugated biomolecule, is grafted with free monomer onto a hydrophilic, solid-phase, polymeric substrate which has been pre-irradiated with ionizing radiation. The pre-irradiation step is carried out, preferably at -78.degree. C. in air, while the grafting step is carried out at 0.degree. C. in a substantially oxygen-free atmosphere. The technique is applicable to immobilization of a wide variety of biomolecules, such as enzymes, catalysts, hormones, lectins, drugs, vitamins, antibodies, antigens, nucleic acids, DNA and RNA segments, pesticides, dyes and fertilizers. The products may be used for therapeutic or diagnostic applications or bioseparations.
Inventor(s): Hoffman; Allan S. (Seattle, WA), Dong; Liang C. (Seattle, WA)
Assignee: Board of Regents of the University of Washington (Seattle, WA)
Application Number:07/348,049
Patent Claims:1. A method of producing an immobilized biomolecule comprising the steps of:

exposing a solid-phase polymeric substrate to ionizing radiation at a temperature between about 0.degree. C. to about -78.degree. C.; and

contacting the irradiated substrate with a biomolecule and free monomer, in aqueous solution in a substantially oxygen-free atmosphere at a temperature less than the temperature at which the biomolecule inactivates, to form a graft copolymer, wherein the biomolecule is immobilized at least in part within the graft copolymer.

2. The method of claim 1 wherein the biomolecule is an enzyme.

3. The method of claim 2 wherein the enzyme is asparaginase.

4. The method of claim 1 wherein the biomolecule is a catalyst.

5. The method of claim 1 wherein the biomolecule is an antibody.

6. The method of claim 1 wherein the biomolecule is an antigen.

7. The method of claim 1 wherein the biomolecule is a drug.

8. The method of claim 1 wherein the biomolecule is a hormone.

9. The method of claim 1 wherein the biomolecule is a pesticide.

10. The method of claim 1 wherein the biomolecule is a fertilizer.

11. The method of claim 1 wherein the biomolecule is an insecticide.

12. The method of claim 1 wherein the biomolecule is a dye.

13. The method of claim 1 wherein the biomolecule is a vitamin.

14. The method of claim 1 wherein the biomolecule is a single or double stranded sequence of DNA or RNA.

15. The method of claim 1 wherein the biomolecule is a nucleic acid.

16. The method of claim 1 wherein the substrate is hydrophilic.

17. The method of claim 1 wherein the substrate is a polysaccharide.

18. The method of claim 1 wherein the substrate is selected from the group consisting of cellulose, agarose, sepharose and cross-linked dextran.

19. The method of claim 1 wherein the source of ionizing radiation is .sup.60 Co.

20. The method of claim 1 wherein the source of ionizing radiation is X-rays.

21. The method of claim 1 wherein the source of ionizing radiation is high-energy electrons.

22. The method of claim 1 wherein the source of ionizing radiation is radio frequency plasma gas discharge.

23. The method of claim 1 wherein a substrate is exposed to ionizing radiation at -78.degree. C.

24. The method of claim 1 wherein the monomer is characterized by having at least one vinyl group.

25. The method of claim 1 wherein the monomer is characterized by having at least one allyl group.

26. The method of claim 1 wherein the monomer is selected from the group shown in Table 1.

27. A method of producing an immobilized biomolecule comprising the steps of:

exposing a solid-phase polymeric substrate to ionizing radiation at a temperature between about 0.degree. C. to about -78.degree. C.; and

contacting the irradiated substrate with a monomer-conjugated biomolecule and free monomer, in aqueous solution in a substantially oxygen-free atmosphere at a temperature less than the temperature at which the biomolecule inactivates, to form a graft copolymer, wherein the biomolecule is immobilized at least in part within the graft copolymer.

28. The method of claim 27 wherein the biomolecule is an enzyme.

29. The method of claim 28 wherein the enzyme is asparginase.

30. The method of claim 27 wherein the biomolecule is a catalyst.

31. The method of claim 27 wherein the biomolecule is an antibody.

32. The method of claim 27 wherein the biomolecule is an antigen.

33. The method of claim 27 wherein the biomolecule is a drug.

34. The method of claim 27 wherein the biomolecule is a hormone.

35. The method of claim 27 wherein the biomolecule is a pesticide.

36. The method of claim 27 wherein the biomolecule is a fertilizer.

37. The method of claim 27 wherein the biomolecule is an insecticide.

38. The method of claim 27 wherein the biomolecule is a dye.

39. The method of claim 27 wherein the biomolecule is a vitamin.

40. The method of claim 27 wherein the biomolecule is a single or double stranded sequence of DNA or RNA.

41. The method of claim 27 wherein the biomolecule is a nucleic acid.

42. The method of claim 27 wherein the substrate is hydrophilic.

43. The method of claim 27 wherein the substrate is a polysaccharide.

44. The method of claim 27 wherein the substrate is selected from the group consisting of cellulose, agarose, sepharose and cross-linked dextran.

45. The method of claim 27 wherein the source of ionizing radiation is .sup.60 Co.

46. The method of claim 27 wherein the source of ionizing radiation is X-rays.

47. The method of claim 27 wherein the source of ionizing radiation is high-energy electrons.

48. The method of claim 27 wherein the source of ionizing radiation is a radio frequency plasma gas discharge.

49. The method of claim 27 wherein a substrate is exposed to ionizing radiation at -78.degree. C.

50. The method of claim 27 wherein the monomer is characterized by having at least one vinyl group.

51. The method of claim 27 wherein the monomer is characterized by having at least one allyl group.

52. The method of claim 278 wherein the monomer is selected from the group shown in Table 1.

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