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Last Updated: March 28, 2024

Claims for Patent: 5,010,010


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Summary for Patent: 5,010,010
Title: Production of human parathyroid hormone from microorganisms
Abstract:The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. Finally, the invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast.
Inventor(s): Gautvik; Kaare M. (Oslo, NO), Alestrom; Peter (Sollihogda, NO), Oven; Tordis B. (Oslo, NO), Gabrielsen; Odd S. (Oslo, NO)
Assignee: Selmer-Sande, A.S. (NO)
Application Number:07/393,851
Patent Claims:1. A DNA sequence encoding Saccharomyces mating factor alpha 1 operably linked to human parathyroid hormone wherein said DNA sequence can stably transform a yeast cell to express and secrete an intact human parathyroid hormone.

2. The DNA sequence of claim 1 wherein said DNA sequence comprises the nucleotide sequence set forth in FIG. 11.

3. The yeast cell of claim 1 wherein said cell is in the genus Saccharomyces.

4. The yeast cell of claim 1 wherein said cell is of the species Saccharomyces cerevisiae.

5. The yeast cell of claim 1 wherein said cell is a budding yeast cell.

6. The human parathyroid hormone of claim 1 wherein said hormone has biological activity substantially equivalent to naturally occurring human parathyroid hormone.

7. A plasmid comprising the DNA sequence of claim 1.

8. A plasmid according to claim 7 wherein said nucleotide sequence comprises: ##STR2##

9. A plasmid according to claim 7 wherein the nucleotide sequence comprises: ##STR3##

10. A plasmid according to claim 7 wherein the nucleotide sequence comprises: ##STR4##

11. A plasmid according to claim 7 wherein the nucleotide sequence comprises: ##STR5##

12. A microorganism containing the plasmid of claim 8.

13. A microorganism according to claim 12 wherein said microorganism is Escherichia coli.

14. A microorganism containing the plasmid of claim 9.

15. A microorganism according to claim 14 wherein the microorganism is Escherichia coli.

16. A microorganism containing the plasmid of claim 10.

17. A microorganism according to claim 16 wherein the microorganism is Escherichia coli.

18. A microorganism containing the plasmid of claim 11.

19. A microorganism according to claim 18 wherein the microorganism is Escherichia coli.

20. A plasmid according to claim 7, wherein the nucleotide sequence comprises: ##STR6##

21. The plasmid of claim 7, wherein the nucleotide sequence comprises: ##STR7##

22. A microorganism containing the plasmid of claim 7.

23. A transformed yeast cell comprising a DNA sequence encoding Saccharomyces mating factor alpha 1 operably linked to human parathyroid hormone, said cell capable of expressing said DNA and secreting said expressed DNA into an extracellular environment, whereby said secreted, expressed DNA is intact human parathyroid hormone.

24. The transformed yeast cell of claim 23 wherein said DNA sequence comprises the nucleotide sequence of FIG. 11.

25. The transformed yeast cell of claim 23 wherein said yeast cell is of the genus Saccharomyces.

26. The transformed yeast cell of claim 23 wherein said yeast cell is of the species Saccharomyces cerevisiae.

27. The transformed yeast cell of claim 23 wherein said yeast cell is a budding yeast cell.

28. The transformed cell of claim 23 wherein said expressed human parathyroid hormone has a biological activity substantially equivalent to naturally occurring human parathyroid hormone.

29. A DNA sequence comprising a vector capable of stably transforming yeast wherein said DNA sequence encodes Saccharomyces mating factor alpha 1 operably linked to human parathyroid hormone wherein said vector can stably transform a yeast cell to express and secrete an intact human parathyroid hormone.

30. The transformed yeast cell of claim 29 wherein said yeast cell is a budding yeast.

31. The vector of claim 30 wherein said vector is an autonomous replicating plasmid.

32. The vector of claim 30 wherein said vector is an integrating plasmid.

33. The transformed yeast of claim 30 wherein said yeast is from the genus Saccharomyces.

34. The transformed yeast of claim 30 wherein said yeast is of the species Saccharomyces cerevisiae.

35. A plasmid, pSSHPTH-10, deposited in the American Type Culture Collection under ATCC No. 40267.

36. A transformed E. coli containing pSSHPTH-10, deposited in the American Type Culture Collection under ATCC No. 67223.

37. A transformed S. cerevisiae containing pSS.alpha.L.times.5-HPTH1, deposited in the American Type Culture Collection under ATCC No. 20821.

38. A plasmid, pSS.alpha.L.times.5-HPTH1, deposited in the American Type Culture Collection under ATCC No. 40266.

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