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Last Updated: April 23, 2024

Claims for Patent: 4,849,407

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Summary for Patent: 4,849,407

Title:	Biologically active mosaic proteins
Abstract:	Dimeric proteins having substantially the same biological activity as PDGF are disclosed. More specifically, the proteins may have two polypeptide chains, one of the chains being a mosaic of amino acid sequences substantially identical to portions of the A- or B-chains of PDGF, the second of the chains being substantially homologous to either the A-chain or the B-chain of PDGF, the proteins being chemotactic or mitogenic for fibroblasts. Alternatively, each of the two polypeptide chains may be a mosaic of amino acid sequences as described above. Therapeutic compositions containing these proteins and methods for enhancing the wound-healing process in warm-blooded animals are also disclosed.
Inventor(s):	Murray; Mark J. (Seattle, WA), Kelly; James D. (Seattle, WA)
Assignee:	ZymoGenetics, Inc. (Seattle, WA)
Application Number:	06/941,970
Patent Claims:	1. A protein having two polypeptide chains, one of said chains being a mosaic of amino acid sequences substantially identical to portions of the A- or B-chains of PDGF, the second of said chains being substantially homologous to the A-chain of PDFG, said protein being chemotactic or mitogenic for fibroblasts, wherein said mosaic chain comprises a sequence selected from the group consisting of: (a) A-chain amino acids 1-17 fused in reading frame to B-chain amino acids 24-109; and (b) A-chain amino acids 1-17 fused in reading frame to B-chain amino acids 24-97 fused in reading frame to A-chain amino acids 92-104; and wherein said second chain comprises a sequence selected from the group consisting of: (a) the amino acid sequence of the A-chain of PDGF from amino acid 15 to amino acid 104; (b) the amino acid sequence of the A-chain of PDGF from amino acid 23 to amino acid 104; (c) the amino acid sequence of the A-chain of PDGF from amino acid 9 to amino acid 95; (d) the amino acid sequence of the A-chain of PDGF from amino acid 23 to amino acid 95; (e) the amino acid sequence of the A-chain of PDGF from amino acid 1 to amino acid 95; and (f) the amino acid sequence of the A-chain of PDGF from amino acid 1 to amino acid 104. 2. A protein having two polypeptide chains, one of said chains being a mosaic of amino acid sequences substantially identical to portions of the A- or B-chains of PDGF, the second of said chains being substantially homologous to the B-chain of PDGF, said protein being chemotactic or mitogenic for fibroblasts, wherein said mosaic chain comprises a sequence selected from the group consisting of: (a) A-chain amino acids 1-17 fused in reading frame to B-chain amino acids 24-109; and (b) A-chain amino acids 1-17 fused in reading frame to B-chain amino acids 24-97 fused in reading frame to A-chain amino acids 92-104; and wherein said second chain comprises a sequence selected from the group consisting of: (a) the amino acid sequence of the B-chain of PDGF from amino acid 15 to amino acid 109; (b) the amino acid sequence of the B-chain of PDGF from amino acid 29 to amino acid 109; (c) the amino acid sequence of the B-chain of PDGF from amino acid 15 to amino acid 101; (d) the amino acid sequence of the B-chain of PDGF from amino acid 29 to amino acid 101; (e) the amino acid sequence of the B-chain of PDGF from amino acid 1 to amino acid 101; and (f) the amino acid sequence of the B-chain of PDGF from amino acid 1 to amino acid 109. 3. A protein having two polypeptide chains, each of said chains being a mosaic of amino acid sequences selected from the group consisting of: (a) A-chain amino acids 1-17 fused in reading frame to B-chain amino acids 24-109; and (b) A-chain amino acids 1-17 fused in reading frame to B-chain amino acids 24-97 fused in reading frame to A-chain amino acids 92-194. 4. A wound-healing composition comprising a protein according to claim 1, in combination with a physiologically acceptable carrier or diluent. 5. A wound-healing composition comprising a protein according to claim 2, in combination with a physiologically acceptable carrier or diluent. 6. A wound-healing composition comprising a protein according to claim 3, in combination with a physiologically acceptable carrier or diluent. 7. The wound-healing composition of claim 4, 5 or 6 wherein the carrier or diluent is selected from the group consisting of albumin, saline and sterile water. 8. The wound-healing composition of claim 4, 5 or 6, including an adjuvant. 9. The wound-healing composition of claim 8 wherein the adjuvant is selected from the group consisting of collagen, hyaluronic acid preparations, fibronectin and factor XIII. 10. A method for enhancing the wound-healing process in warm-blooded animals, comprising administering to a warm-blooded animal an effective amount of a wound-healing composition according to claim 4, 5 or 6.

Details for Patent 4,849,407

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006	⤷ Try a Trial	2004-10-12
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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