Claims for Patent: 4,752,578
✉ Email this page to a colleague
Summary for Patent: 4,752,578
Title: | Collagenase inducing factor |
Abstract: | A method for the production of collagenase inducing factor is disclosed which comprises growing the human liver adenocarcinoma cell line SK-HEP-1 in nutrient culture medium at 35.degree.-38.degree. C. and recovering the resulting collagenase inducing factor from the spent cells or conditioned medium. |
Inventor(s): | Moore; William M. (St. Charles, MO), Spilburg; Curtis A. (Chesterfield, MO) |
Assignee: | Monsanto Company (St. Louis, MO) |
Application Number: | 06/851,039 |
Patent Claims: | 1. Method for the production of collagenase inducing factor that stimulates synthesis of synovial cell collagenase and prostaglandin E.sub.2 comprising growing the human liver
adenocarcinoma cell line SK-HEP-1 in nutrient culture media at 35.degree.-38.degree. C. and recovering the resulting collagenase inducing factor from the spent cells or conditioned medium.
2. The method of claim 1 in which the collagenase inducing factor is recovered from the spent cells by freezing and thawing said cells suspended in low ionic buffer followed by subjecting the resulting cell lysate to the stepwise sequence of chromatofocusing with Polybuffer exchanger, Phenyl-Sepharose chromatography, and Bio-Gel P-30 gel filtration chromatography. 3. The method of claim 1 in which the collagenase inducing factor is recovered from the conditioned medium by the stepwise sequence of ammonium sulfate fractionation, Affi-Gel Blue chromatography, Bio-Gel HTP chromatography, and Sephadex G-75 gel filtration chromatography. |
Details for Patent 4,752,578
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Smith & Nephew, Inc. | SANTYL | collagenase | Ointment | 101995 | 06/04/1965 | ⤷ Try a Trial | 2039-03-29 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
Make Better Decisions: Try a trial or see plans & pricing
Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.