Claims for Patent: 4,687,740

Summary for Patent: 4,687,740

Title:	Decapeptides produced from bioadhesive polyphenolic proteins
Abstract:	Methods are described for the preparation and isolation of novel decapeptides of the formula: ##STR1## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, wherein each R is independently selected from the group containing hydrogen and methyl, from bioadhesive polyphenolic proteins which comprise: ##STR2## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl. Such decapeptides may be used to construct large polyphenolic molecules comprising from about 1 to about 1000 decapeptide repeating units and wherein the linking group is selected from the group comprising amino acid, oligopeptide and bifunctional spacer.
Inventor(s):	Waite; J. Herbert (Collinsville, CT)
Assignee:	University of Connecticut Research & Development Corp. (Farmington, CT)
Application Number:	06/820,143
Patent Claims:	1. A method for the preparation of decapeptides of the formula: ##STR10## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of: (a) isolating bioadhesive proteins from mussel phenol glands; (b) enzymatically digesting said bioadhesive protein; and (c) recovering said decapeptides. 2. A method, as claimed in claim 1, which enzymatic digestion step comprises digesting said bioadhesive protein with trypsin. 3. A method, as claimed in claim 1, which enzymatic digestion step comprises: (a) digesting said bioadhesive protein with clostridial collagenase; and (b) digesting the resultant collagenase-resistant fragment with trypsin. 4. A method for the preparation of decapeptides of the formula: ##STR11## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of: (a) isolating from marine mussels bioadhesive polyphenolic proteins which comprise: ##STR12## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; (b) digesting said bioadhesive proteins with clostridial collagenase; (c) digesting the resultant collagenase-resistant fragment with trypsin; and (d) recovering said decapeptides. 5. A method for the preparation of decapeptides of the formula: ##STR13## wherein each x is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen amd methyl which comprises the steps of: (a) isolating from marine mussles bioadhesive polyphenolic proteins which comprise: ##STR14## wherein n is a whole number from about 60 to about 100, and wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl; (b) digesting the bioadhesive proteins with trypsin; and (c) recovering said decapeptides. 6. A method, as claimed in claim 2, wherein the isolation of said bioadhesive proteins comprises the steps of: a. disecting mussel phenol glands; b. homogenizing said glands in neutral buffer; c. centrifuging the resultant homogenized gland to a pellet; d. homogenizing said pellet in cold-dilute acetic acid; e. centrifuging said homogenized pellet; and f. recovering the bioadhesive protein from the resultant supernatant.

Details for Patent 4,687,740

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2004-08-18