➤ Get the DrugPatentWatch Daily Briefing

Get Daily Updates on Generic Entry, Litigation, Biosimilars, and more …

Serving leading biopharmaceutical companies globally:

Moodys
Boehringer Ingelheim
Baxter
Dow
Medtronic
McKesson

Last Updated: October 21, 2021

DrugPatentWatch Database Preview

Claims for Patent: 4,687,740

➤ Get the DrugPatentWatch Daily Briefing

« Back to Dashboard

Summary for Patent: 4,687,740
Title: Decapeptides produced from bioadhesive polyphenolic proteins
Abstract:Methods are described for the preparation and isolation of novel decapeptides of the formula: ##STR1## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, wherein each R is independently selected from the group containing hydrogen and methyl, from bioadhesive polyphenolic proteins which comprise: ##STR2## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl. Such decapeptides may be used to construct large polyphenolic molecules comprising from about 1 to about 1000 decapeptide repeating units and wherein the linking group is selected from the group comprising amino acid, oligopeptide and bifunctional spacer.
Inventor(s): Waite; J. Herbert (Collinsville, CT)
Assignee: University of Connecticut Research & Development Corp. (Farmington, CT)
Application Number:06/820,143
Patent Claims:1. A method for the preparation of decapeptides of the formula: ##STR10## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of:

(a) isolating bioadhesive proteins from mussel phenol glands;

(b) enzymatically digesting said bioadhesive protein; and

(c) recovering said decapeptides.

2. A method, as claimed in claim 1, which enzymatic digestion step comprises digesting said bioadhesive protein with trypsin.

3. A method, as claimed in claim 1, which enzymatic digestion step comprises:

(a) digesting said bioadhesive protein with clostridial collagenase; and

(b) digesting the resultant collagenase-resistant fragment with trypsin.

4. A method for the preparation of decapeptides of the formula: ##STR11## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of:

(a) isolating from marine mussels bioadhesive polyphenolic proteins which comprise: ##STR12## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl;

(b) digesting said bioadhesive proteins with clostridial collagenase;

(c) digesting the resultant collagenase-resistant fragment with trypsin; and

(d) recovering said decapeptides.

5. A method for the preparation of decapeptides of the formula: ##STR13## wherein each x is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen amd methyl which comprises the steps of:

(a) isolating from marine mussles bioadhesive polyphenolic proteins which comprise: ##STR14## wherein n is a whole number from about 60 to about 100, and wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl;

(b) digesting the bioadhesive proteins with trypsin; and

(c) recovering said decapeptides.

6. A method, as claimed in claim 2, wherein the isolation of said bioadhesive proteins comprises the steps of:

a. disecting mussel phenol glands;

b. homogenizing said glands in neutral buffer;

c. centrifuging the resultant homogenized gland to a pellet;

d. homogenizing said pellet in cold-dilute acetic acid;

e. centrifuging said homogenized pellet; and

f. recovering the bioadhesive protein from the resultant supernatant.

Make Better Decisions: Try a trial or see plans & pricing

Serving leading biopharmaceutical companies globally:

McKinsey
McKesson
Moodys
Colorcon
Johnson and Johnson
Harvard Business School

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.