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Last Updated: March 28, 2024

Claims for Patent: 4,671,954


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Summary for Patent: 4,671,954
Title: Microspheres for incorporation of therapeutic substances and methods of preparation thereof
Abstract:Novel hydrophilic protein or polypeptide microspheres prepared by dispersing an aqueous solution or dispersion of protein or polypeptide in an organic solvent solution of a high molecular weight polymer to form a stabilized dispersion of microspheres and cross-linking said microspheres with a polyfunctional cross-linking agent.
Inventor(s): Goldberg; Eugene P. (Gainesville, FL), Longo; William E. (Gainesville, FL), Iwata; Hiroo (Suita, JP)
Assignee: University of Florida (Gainesville, FL)
Application Number:06/825,789
Patent Claims:1. A method of preparing novel hydrophilic microspheres consisting essentially of cross-linked protein or polypeptide comprising

(a) providing a dispersion of an aqueous solution or dispersion of polypeptide or protein microspheres in an organic, substantially water immiscible solvent solution of a high molecular weight polymer, said organic solvent being substantially a non-solvent for said microspheres and said polymer solution stabilizing the dispersion of microspheres,

(b) incorporating a polyfunctional cross-linking agent for said protein or polypeptide in the organic phase of said dispersion, and

(c) allowing said cross-linking agent to react with said protein or polypeptide microspheres for a time sufficient to cross-link at least a portion of the microspheres, thereby providing microspheres containing free reactive functional groups.

2. The method of claim 1 including the step of separating said cross-linked protein or polypeptide microspheres from said dispersion.

3. The method of claim 1 wherein said protein is albumin.

4. The method of claim 3 wherein said albumin is human serum albumin.

5. The method of claim 3 wherein said albumin is bovine serum albumin.

6. The method of claim 1 or 2 wherein said organic solvent solution of high molecular weight polymer is a solution of polymethylmethacrylate in a mixture of toluene and chloroform.

7. The method of claim 1 or 2 wherein said organic solvent solution of high molecular weight polymer is a solution of polyoxyethylene-polyoxypropylene copolymer in chloroform.

8. The method of claim 1 or 2 wherein said organic solvent solution of high molecular weight polymer is a solution of cellulose acetate butyrate in ethylene dichloride.

9. The method of claim 1 or 2 wherein said organic solvent solution of high molecular weight polymer is a solution of bisphenol polycarbonate in chloroform.

10. The method of claim 1 or 2 wherein the concentration of high molecular weight polymer in organic solvent is from about 1% to about 40%, by weight.

11. The method of claim 1 or 2 wherein said stabilized dispersion of protein or polypeptide microspheres is formed by dispersing an aqueous solution of said protein or polypeptide in said organic solvent solution of high molecular weight polymer.

12. The method of claim 1 or 2 wherein said microspheres have an average size in the range of from about 0.05 microns to about 500 microns.

13. The method of claim 1 or 2 wherein said cross-linking agent is a dialdehyde.

14. The method of claim 13 wherein said cross-linking agent is glutaraldehyde.

15. The method of claim 1 or 2 wherein said cross-linking agent is a diisocyanate.

16. The method of claim 15 wherein said diisocyanate is 2,4-tolylene diisocyanate or 1,6-diisocyanate hexane.

17. The product produced according to the method of claim 1 or 2.

18. The product produced according to the method of claim 3.

19. The method of claim 1 or 2 including the step of reacting reactive functional groups of said cross-linked microspheres with a first substance containing at least one functional group reactive therewith to form a covalent bond between said cross-linked microspheres and said substance.

20. The method of claim 19 wherein said first substance is a biologically active substance suitable for administration to a biological system.

21. The method of claim 19 wherein said substance contains at least one additional functional group which is non-reactive with the reactive functional groups of said cross-linked microspheres.

22. The method of claim 21 wherein said at least one additional functional group is reacted with a second substance containing a functional group reactive therewith to form a bond therebetween.

23. The method of claim 22 wherein said second substance is a biologically active substance suitable for administration to a biological system.

24. The product produced according to the method of claim 19.

25. The product produced according to the method of claim 20.

26. The product produced according to the method of claim 21.

27. The product produced according to the method of claim 22.

28. The product produced according to the method of claim 23.

29. A composition in unit dosage form adapted for administration to a biological system comprising a biologically effective amount of the product of any of claims 24 to 28 and a biologically acceptable carrier therefor.

30. A method of administering a biologically active substance to a biological system comprising administering thereto the product of any of claims 24 to 28.

Details for Patent 4,671,954

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Grifols Therapeutics Llc ALBUKED, PLASBUMIN-20, PLASBUMIN-25, PLASBUMIN-5 albumin (human) For Injection 101138 10/21/1942 ⤷  Try a Trial 2004-06-09
Baxalta Us Inc. BUMINATE, FLEXBUMIN albumin (human) Injection 101452 03/03/1954 ⤷  Try a Trial 2004-06-09
Csl Behring Ag ALBURX albumin (human) Injection 102366 07/23/1976 ⤷  Try a Trial 2004-06-09
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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