Claims for Patent: 4,624,924

Summary for Patent: 4,624,924

Title:	Novel collagenase \"discolysin\" and production method thereof
Abstract:	Disclosed herein is a novel collagenase \"discolysin\". It is useful for biochemical researches and the treatment of many diseases caused by collagen, such as hernia of intervertebral disc. It can be produced by culturing a discolysin-producing bacterium belonging to genus of Streptomyces in a culture medium and then collecting discolysin from the culture medium.
Inventor(s):	Endo; Akira (Tokyo, JP)
Assignee:	Kabushiki Kaisha Yakult Honsha (Tokyo, JP)
Application Number:	06/708,637
Patent Claims:	1. A collagenase "discolysin" having the following physical and chemical properties and produced by culturing a bacterium belonging to the genus of Streptomyces in a culture medium and then collecting discolysin from the culture medium: (1) Molecular weight: 60,000-70,000 (SDS-Gel electrophoresis); 90,000-110,000 (SDS-Gel electrophoresis; presence of 10 mM EDTA); (2) Formation of two collagenase-active bands by disc gel electrophoresis; (3) Isoelectric point: pH 4.8 and 4.9 (determined by focal electrophoresis); (4) Elemental analysis data: C, about 43%; H, about 7%; N, about 13%; (5) U.V. spectrum: FIG. 1; (6) I.R. spectrum: FIG. 2; (7) Precipitated by 45-81% saturation with ammonium sulfate; (8) Adsorbed on an ion exchanger, DEAE cellulose; (9) Decomposes insoluble collagens and denatured collagen but is extremely inert to casein; (10) Optimum pH for decomposition of insoluble collagens: 7.6-8.0; and (11) Inhibited by EDTA. 2. A method for producing a novel collagenase "discolysin",as recited in claim 1, which comprises culturing a discolysin-producing bacterium belonging to the genus of Streptomyces in a culture medium and then collecting discolysin from the culture medium. 3. A method according to claim 2, wherein the discolysin-producing bacterium is Streptomyces C-51 strain. 4. The method of claim 2, wherein said culturing is conducted aerobically in a culture medium at 25.degree.-35.degree. C.

Details for Patent 4,624,924

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2004-03-09