Claims for Patent: 4,525,456

Summary for Patent: 4,525,456

Title:	Support matrix and immobilized enzyme system
Abstract:	A support matrix which immobilizes enzymes by ion exchange forces comprises a core support deposited on which is a functionalized polyethylenimine insoluble in water. Such support matrices may be prepared from a virtually limitless variety of core supports since the functionalized polyethylenimines of this invention show excellent adhesive properties even to smooth surfaces. Such support matrices are particularly useful where the enzyme to be immobilized has a rather limited inherent half-life. The functionalized polyethylenimine is a carboxylic acid amide of polyethylenimine, a sulfonic acid amide of polyethylenimine or polyalkylated polyethylenimine.
Inventor(s):	Rohrbach; Ronald P. (Forest Lake, IL)
Assignee:	UOP Inc. (Des Plaines, IL)
Application Number:	06/439,958
Patent Claims:	1. A method of preparing a support matrix comprising depositing a water-insoluble functionalized polyethylenimine, where the polyethylenimine has a molecular weight from about 1200 to about 100,000, selected from the group consisting of carboxylic acid amides of polyethylenimine, sulfonic acid amides of polyethylenimine, and polyalkylated polyethylenimine on a core support, and recovering the resulting support matrix. 2. The method of claim 1 where the functionalized polyethylenimine is a carboxylic or sulfonic acid amide of polyethylenimine wherein substantially all primary amino groups and a major proportion of the secondary amino groups have been converted to amides. 3. The method of claim 2 where the carboxylic acid is a saturated aliphatic carboxylic acid containing more than about 4 carbon atoms. 4. The method of claim 2 where the carboxylic acid is an aromatic carboxylic acid. 5. The method of claim 4 where the acid is benzoic acid. 6. The method of claim 2 where the sulfonic acid is an aromatic sulfonic acid. 7. The method of claim 6 where the acid is benzene sulfonic acid. 8. The method of claim 6 where the acid is toluene sulfonic acid. 9. The method of claim 1 where the core support is selected from the group consisting of inorganic oxides, glass, ceramics, metals, plastics and membranes. 10. A support matrix comprising a core support on whose surface is deposited a water-insoluble functionalized derivative of polyethylenimine, where the polyethylenimine has a molecular weight from about 1200 to about 100,000, selected from the group consisting of carboxylic acid amides of polyethylenimine, sulfonic acid amides of polyethylenimine, and polyalkylated polyethylenimne. 11. The support matrix of claim 10 where the functionalized polyethylenimine is a carboxylic or sulfonic acid amide of polyethylenimine wherein substantially all the primary amino groups and a major proportion of the secondary amino groups have been converted to amides. 12. The support matrix of claim 11 where the carboxylic acid is a saturated aliphatic carboxylic acid containing more than about 4 carbon atoms. 13. The support matrix of claim 11 where the carboxylic acid is an aromatic carboxylic acid. 14. The support matrix of claim 13 where the acid is benzoic acid. 15. The support matrix of claim 11 where the sulfonic acid is an aromatic sulfonic acid. 16. The support matrix of claim 15 where the acid is benzene sulfonic acid. 17. The support matrix of claim 15 where the acid is toluene sulfonic acid. 18. The support matrix of claim 10 where the core support is selected from the group consisting of inorganic oxides, glass, ceramics, metals, plastics and membranes. 19. An immobilized enzyme system comprising the support matrix of claim 10 to which there is bound an enzyme. 20. The immobilized enzyme system of claim 19 where the enzyme is selected from the group consisting of glucoamylase, alphaamylase, beta-amylase, pullulanase, lactase, rennin, glucose oxidase, protease, lipase, urease, arginase, asparaginase, catalase, chymotrypsin, cellulase, peroxidase, lysozyme, and papain.

Details for Patent 4,525,456

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2039-03-29