Claims for Patent: 4,494,547

Summary for Patent: 4,494,547

Title:	2H-isoindolediones, their synthesis and use as radiosensitizers
Abstract:	Isoindoledione compounds of the formula: ##STR1## wherein: R.sub.1 and R.sub.3 each separately is phenyl, substituted phenyl, alkyl of 1 to 4 carbons, --CHO, --CH.sub.2 OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, hydrogen, or together with R.sub.2 is a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.2 is phenyl, substituted phenyl, --CH.sub.2 OR.sub.6, --CH.sub.2 CH.sub.2 OR.sub.6, alkyl of 1 to 4 carbons or with either R.sub.1 or R.sub.3 is a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.4 and R.sub.5 which may be the same or different each separately is hydrogen, alkyl of 1 to 4 carbons, --OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, --CHO, --CH.sub.2 OR.sub.6 or together R.sub.4 and R.sub.5 is a butadiene radical which forms a benzene ring; R.sub.6 is hydrogen or alkyl of 1 to 4 carbons; provided that R.sub.1 and R.sub.3 are not both phenyl and that when R.sub.4 and R.sub.5 are both hydrogen, R.sub.1, R.sub.2 and R.sub.3 are not all methyl. The compounds are useful as radio-sensitizers in the therapeutic radiation of cancerous tissues.
Inventor(s):	Myers; John A. (Durham, NC)
Assignee:	North Carolina Central University (Durham, NC)
Application Number:	06/248,793
Patent Claims:	1. An isoindoledione compound of the formula: ##STR13## wherein: R.sub.1 and R.sub.3 each separately is phenyl, substituted phenyl, alkyl of 1 to 4 carbons, --CHO, --CH.sub.2 OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, hydrogen, or together with R.sub.2 is a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.2 is phenyl, substituted phenyl, --CH.sub.2 OR.sub.6, --CH.sub.2 CH.sub.2 OR.sub.6, alkyl of 1 to 4 carbons or with either R.sub.1 or R.sub.3 is a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.4 and R.sub.5 which may be the same or different each separately is hydrogen, alkyl of 1 to 4 carbons, --OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, --CHO, --CH.sub.2 OR.sub.6 or together R.sub.4 and R.sub.5 is a butadiene radical which forms a benzene ring; R.sub.6 is hydrogen or alkyl of 1 to 4 carbons; provided that R.sub.1 and R.sub.3 are not both phenyl and that when R.sub.4 and R.sub.5 are both hydrogen, R.sub.1, R.sub.2 and R.sub.3 are not all methyl. 2. An isoindoledione compound according to claim 1 wherein R.sub.1 and R.sub.3 are selected from phenyl, alkyl of 1 to 4 carbons, and together with R.sub.2, R.sub.1 or R.sub.3 forms a cyclic ring; R.sub.4 and R.sub.5 which may be the same or different are selected from hydrogen, alkyl of 1 to 4 carbons, and methoxy. 3. A compound according to claim 1 selected from the group consisting of: 1,2-dimethyl-3-phenyl-2H-benz[f]isoindole-4,9-dione; 1-phenyl-2,3-trimethylene-2H-benz[f]isoindole-4,9-dione; 1,2,5-trimethyl-3-phenyl-2H-isoindole-4,7-dione; 1,2,6-trimethyl-3-phenyl-2H-isoindole-4,7-dione; 5-methoxy-1,2,6-trimethyl-3-phenyl-2H-isoindole-4,7-dione; 6-methoxy-1,2,5-trimethyl-3-phenyl-2H-isoindole-4,7-dione; 5-methyl-1-phenyl-2,3-trimethylene-2H-isoindole-4,7-dione; 6-methyl-1-phenyl-2,3-trimethylene-2H-isoindole-4,7-dione; 5-methoxy-6-methyl-3-phenyl-1,2-trimethylene-2H-isoindole-4,7-dione; 6-methoxy-5-methyl-3-phenyl-1,2-trimethylene-2H-isoindole-4,7-dione; 1-methyl-2,3-trimethylene-2H-benz[f]isoindole-4,9-dione; 2-methyl-1-phenyl-2H-benz[f]isoindole-4,9-dione; 2,5-dimethyl-1-phenyl-2H-isoindole-4,7-dione; 2,6-dimethyl-1-phenyl-2H-isoindole-4,7-dione; 1,5-dimethyl-2,3-trimethylene-2H-isoindole-4,7-dione; 1,6-dimethyl-2,3-trimethylene-2H-isoindole-4,7-dione; 1-carboethoxy-5-methyl-2,3-trimethylene-2H-isoindole-4,7-dione; 1-carboethoxy-6-methyl-2,3-trimethylene-2H-isoindole-4,7-dione; 1-carboethoxy-2,5-dimethyl-3-phenyl-2H-isoindole-4,7-dione; 1-carboethoxy-2,6-dimethyl-3-phenyl-2H-isoindole-4,7-dione; 5-methyl-1,2-trimethylene-2H-isoindole-4,7-dione; 6-methyl-1,2-trimethylene-2H-isoindole-4,7-dione; and 1,2,3,5-tetramethyl-2H-isoindole-4,7-dione. 4. A method for the preparation of 2H-isoindolediones by the 1,3-dipolar addition of oxazolium-5-oxides to 1,4-quinones which comprises the steps of: (a) generating, at a temperature of from about 20.degree. to about 80.degree. C. in the presence of a non-polar aprotic solvent and a dehydration agent, an oxazolium 5-oxide of the formula: ##STR14## from an N-acyl amino acid of the formula ##STR15## (b) reacting the oxazolium 5-oxide obtained in step (a) with a 1,4-quinone of the formula ##STR16## in the presence of a non-polar aprotic solvent for a period of time sufficient to produce a product of the formula ##STR17## wherein: R.sub.1 and R.sub.3 each separately is phenyl, substituted phenyl, alkyl of 1 to 4 carbons, --CHO, --CH.sub.2 OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, hydrogen, or together with R.sub.2 are a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.2 is phenyl, substituted phenyl, --CH.sub.2 OR.sub.6, --CH.sub.2 CH.sub.2 OR.sub.6, alkyl of 1 to 4 carbons or with either R.sub.1 or R.sub.3 is a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.4 and R.sub.5 which may be the same or different is hydrogen, alkyl of 1 to 4 carbons, --OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, --CHO, --CH.sub.2 OR.sub.6 or together R.sub.4 and R.sub.5 are a butadiene radical which form a benzene ring; R.sub.6 is hydrogen or alkyl of 1 to 4 carbons; and (c) separating and recovering the product from the solvent solution. 5. The method of claim 4 wherein the dehydration agent is acetic anhydride or dicyclohexylcarbodiimide. 6. The method of claim 4 wherein step (b) is carried out at a temperature ranging from about 20.degree. to about 55.degree. C. 7. The method of claim 4 or 5 wherein steps (a) and (b) are carried out in an inert atmosphere. 8. The method of claim 5 wherein said agent is acetic anhydride and prior to step (b) the reaction solution is neutralized by the addition of sodium carbonate and sodium sulfate. 9. The method of claim 4 wherein R.sub.1 and R.sub.2 each separately is selected from the group consisting of phenyl, methyl R.sub.3 is methyl or phenyl; R.sub.4 is hydrogen, or methoxy; and R.sub.5 is hydrogen, or methyl. 10. The method of claim 4 wherein said 1,4-quinone is selected from the group consisting of 1,4-benzoquinone; 1,4-naphthoquinone; 2-methyl-1,4-benzoquinone and 2-methyl-3-methoxy-1,4-benzoquinone. 11. The method of claim 4 or 10 wherein said oxazolium 5-oxide is selected from the group consisting of 3-methyl-2,4-diphenyloxazolium-5-oxide; 2,3-dimethyl-4-phenyloxazolium-5-oxide and 2-phenyl-3,4-trimethyleneoxazolium-5-oxide. 12. A radiosensitizing composition for use in the radiotherapeutic treatment of cancerous tissues which comprises as an active ingredient an isoindoledione compound of the formula ##STR18## wherein: R.sub.1 and R.sub.3 each separately is phenyl, substituted phenyl, alkyl of 1 to 4 carbons, --CHO, --CH.sub.2 OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, hydrogen, or together with R.sub.2 are a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.2 is phenyl, substituted phenyl, --CH.sub.2 OR.sub.6, --CH.sub.2 CH.sub.2 OR.sub.6, alkyl of 1 to 4 carbons or with either R.sub.1 or R.sub.3 is a divalent alkyl or alkenyl group of 3 to 5 carbons which form a cyclic ring; R.sub.4 and R.sub.5 which may be the same or different is hydrogen, alkyl of 1 to 4 carbons, --OR.sub.6, --CO.sub.2 R.sub.6, --COR.sub.6, --CHO, --CH.sub.2 OR.sub.6 or together R.sub.4 and R.sub.5 are a butadiene radical which form a benzene ring; R.sub.6 is hydrogen or alkyl of 1 to 4 carbons; together with a pharmaceutically acceptable carrier. 13. An injectable composition according to claim 12, wherein said carrier is selected from the group consisting of sterile buffered saline solution and sterile water. 14. The composition of claim 12 in the form of a tablet, capsule, syrup or elixir. 15. A method for the radiotherapeutic treatment of cancerous tissues in mammals which comprises: (a) administering to said mammal, in an amount effective to enhance subsequent radiation of cancerous tissue, a radiosensitizing composition as claimed in claim 12; and (b) subsequently exposing the cancerous tissue to radiation whereby enhanced radiation of the tissue is achieved. 16. The method of claim 13 wherein the amount of said composition administered ranges from about 0.1 to 0.8 mg of active ingredient per kg body weight of the mammal. 17. The method of claim 16 wherein the amount of said composition administered is about 0.4 mg per kg of body weight. 18. The method of claim 12 wherein said radiation is gamma radiation. 19. The method of claim 12 wherein the time between steps (a) and (b) is from between about 15 to about 60 minutes.

Details for Patent 4,494,547

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006		2039-09-23