Claims for Patent: 4,478,000

Summary for Patent: 4,478,000

Title:	Process for stimulating asexual propagation of Simmondsia chinensis tissue
Abstract:	The present invention embodies a process for mass propagation of plant tissue preferably of a Simmondsia chinensis, commonly known as Jojoba, and plant tissue having similar characteristics thereto. The process is directed to asexual reproduction of plantlets from tissue of immature and mature plants from a single piece of donor tissue and involves performing the steps thereon of: sterilization; chemical pretreatment with a cytokinin to increase the likelihood of tissue survival; washing again in sterile water; placing and retaining for a specified period the prepared tissue on first and second culture media to support and stimulate tissue and shoot development; separating the shoots by surgical division and transferring to a shoot multiplication medium for further development for a desired period; then transferring to a rooting medium for development into plantlets which are then transferred to a soil mix. The process of the present invention including the steps of the chemical pretreatment soak in a cytokinin of preferred concentrations; the use of certain chemicals in the culture media are complementary to the pretreatment soak and provide for an increase in plantlet survival and multiplication over former processes.
Inventor(s):	Wochok; Zachary S. (Manchester, MO), Sluis; Carolyn J. (Davis, CA)
Assignee:	Plant Resources Institute (Salt Lake City, UT)
Application Number:	06/446,378
Patent Claims:	1. A process for initiating mass propagation of plant tissue from undifferentiated Simmondsia chenensis plant tissue comprising the steps of, sterilization of the donor tissue; pretreatment of said tissue with a solution containing a cytokinin that is N.sub.6 -benzyladenine; placing and culturing said treated tissue on a first culture medium appropriate to support and promote tissue growth under appropriate conditions of temperature and light; further culturing the tissue on a second culture medium compatible with the pretreatment step to promote additional tissue growth and shoot development; separating the developed tissue into individual shoots; placing and culturing said shoots on a shoot multiplication medium; separating the multiplied shoots into individual shoots for development on a root initiation medium into plantlets; and transplanting the plantlets into a soil mix. 2. A process for initiating mass propagation of plant tissue as recited in claim 1, wherein the plant tissue is sterilized by agitating it in a solution containing sodium hypochlorite and TWEEN-20. 3. A process for initiating mass propagation of plant tissue as recited in claim 1, wherein the plant tissue is sterilized by agitating it in a solution containing hydrogen peroxide and TWEEN-20. 4. A process for initiating mass propagation of plant tissue as recited in claim 1, wherein the pretreatment solution is maintained at a pH of 7.0.+-.0.5. 5. A process for initiating mass propagation of plant tissue as recited in claim 1, wherein the N.sub.6 -benzyladenine concentration is in a range from 200 ppm to 400 ppm in a sterile water solution. 6. A process for initiating mass propagation of plant tissue as recited in claim 1, further including, after pretreating said tissue, the step of at least one washing of the tissue in a sterile water solution. 7. A process for initiating mass propagation of plant tissue as recited in claim 1, wherein the shoot multiplication medium consists of a modified Murashige and Skoog basal salts medium and N.sub.6 -benzyladenine(BA)/naphtalene acetic acid(NAA) in relative concentrations, in milligrams per liter, that range from 0.5BA/0.0NAA to 1.0BA/1.0NAA. 8. A process for initiating mass propagation of plant tissue as recited in claim 1, further including the step of applying a fixing solution of emollient waxes to the plantlet at the time of transfer to a soil mix. 9. A process for initiating mass propagation of plant tissue from undifferentiated Simmondsia chenensis plant tissue comprising the steps of, sterilization of the donor tissue; pretreatment of said tissue with a solution containing a cytokinin that is 6-gamma-gamma-dimethyallyl amino purine; placing and culturing said treated tissue on a first culture medium appropriate to support and promote tissue growth under appropriate conditions of temperature and light; further culturing the tissue on a second culture medium compatible with the pretreatment step to promote additional tissue growth and shoot development; separating the developed tissue into individual shoots; placing and culturing said shoots on a shoot multiplication medium; separating the multiplied shoots into individual shoots for development on a root initiation medium into plantlets; and transplanting the plantlets into a soil mix. 10. A process for initiating mass propagation of plant tissue as recited in claim 9, wherein the plant tissue is sterilized by agitating it in a solution containing sodium hypochlorite and TWEEN-20. 11. A process for initiating mass propagation of plant tissue as recited in claim 9, wherein the plant tissue is sterilized by agitating it in a solution containing hydrogen peroxide and TWEEN-20. 12. A process for initiating mass propagation of plant tissue as recited in claim 9, wherein the pretreatment solution is maintained at a pH of 7.0.+-.0.5. 13. A process for initiating mass propagation of plant tissue as recited in claim 9, wherein the 6-gamma-gamma-dimethyally amino purine concentration is in a range from 200 ppm to 400 ppm in a sterile water solution. 14. A process for initiating mass propagation of plant tissue as recited in claim 9, further including, after pretreating said tissue, the step of at least one washing of the tissue in a sterile water solution. 15. A process for initiating mass propagation of plant tissue as recited in claim 9, wherein the shoot multiplication medium consists of a modified Murashige and Skoog basal salts medium and N.sub.6 -benzyladenine(BA)/naphtalene acetic acid(NAA) in relative concentrations, in milligrams per liter, that range from 0.5BA/0.0NAA to 1.0BA/1.0NAA. 16. A process for initiating mass propagation of plant tissue as recited in claim 9, further including the step of applying a fixing solution of emollient waxes to the plantlet at the time of transfer to a soil mix.

Details for Patent 4,478,000

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006		2001-10-23