Claims for Patent: 4,415,663

Summary for Patent: 4,415,663

Title:	Support matrix for immobilized enzymes
Abstract:	A support matrix comprising a porous support impregnated with a polyamine substantially all of whose nitrogens bear pendant epoxide groups can be readily used to immobilize enzymes. Because immobilization results from formation of a hydrolytically stable carbon-nitrogen single bond, the resulting immobilized enzyme system may be advantageously used where hydrolytic instability of the bound enzyme is an important consideration.
Inventor(s):	Symon; Ted (Lombard, IL), Barszcz; Chester F. (Chicago, IL)
Assignee:	UOP Inc. (Des Plaines, IL)
Application Number:	06/411,157
Patent Claims:	1. A support matrix comprising a porous support selected from the group consisting of alumina, silica, thoria, magnesia, porous glass, ceramics, and combinations thereof, impregnated with a polyamine selected from the group consisting of poly(ethyleneamines) and epiamines, substantially all of whose nitrogens bear a pendant epoxide group, wherein said epiamines are the reaction products of polyepichlorohydrin with an alkylenediamine containing from 2 to about 10 carbon atoms. 2. The support matrix of claim 1 where the support is alumina. 3. The support matrix of claim 1 where the polyamine is selected from the group consisting of diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentamethylenehexamine, and polyethyleneimine. 4. The support matrix of claim 3 where the polyamine is polyethyleneimine. 5. The support matrix of claim 1 where the alkylenediamine is selected from the group consisting of ethylenediamine, 1,2-diaminopropane, and 1,3-diaminopropane. 6. The support matrix of claim 1 where the group pendant to the nitrogen of the polyamine is a 2,3-epoxypropyl group. 7. A method of making a support matrix comprising impregnating a porous support selected from the group consisting of alumina, silica, thoria, magnesia, porous glass, ceramics, and combinations thereof with a polyamine selected from the group consisting of poly(ethyleneamines) and epiamines wherein said epiamines are the reaction products of polyepichlorohydrin with an alkylenediamine containing from 2 to about 10 carbon atoms, removing excess polyamine, treating the impregnated support with an excess of an epihalohydrin so as to react substantially all of the nitrogens of the polyamine with the epihalohydrin, removing excess unreacted epihalohydrin, contacting the resulting material with base so as to regenerate an epoxide moiety, removing excess base, and recovering the resulting support matrix. 8. The method of claim 7 where the support is alumina. 9. The method of claim 7 where the polyamine is selected from the group consisting of diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentamethylenehexamine, and polyethyleneimine. 10. The method of claim 9 where the polyamine is polyethyleneimine. 11. The method of claim 7 where the epihalohydrin contains from about 3 to about 6 carbon atoms. 12. The method of claim 11 where the epihalohydrin is a 3-halo-1,2-epoxypropane. 13. The method of claim 12 where the epihalohydrin is 3-chloro-1,2-epoxypropane. 14. An immobilized enzyme system comprising the support matrix of claim 1 having an enzyme covalently bonded thereto. 15. The immobilized enzyme system of claim 14 where the enzyme is selected from the group consisting of glucose isomerase, glucoamylase, cholesteroloxidase, alcohol dehydrogenase, amino acid oxidase, arginase, asparaginase, catalase, chymotrypsin, cellulase, collagenase, deoxyribonuclease, ficin, histidase, lactase, peroxidase, lysozyme, gamma amylase, papain, rennin, ribonuclease and urease.

Details for Patent 4,415,663

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2039-03-29
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2039-03-29