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Last Updated: March 29, 2024

Claims for Patent: 4,272,433


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Summary for Patent: 4,272,433
Title: Method for preparing antigen and antibody
Abstract:A method for preparing an antigen comprising a peptide-protein complex which comprises reacting a peptide represented by the general formula (I) ##STR1## wherein m is 0 or 1, as a hapten, with a protein as a carrier in the presence of a dialdehyde represented by the general formula (II) wherein n is an integer of 1 to 5 and a method for preparing an antibody specific to pancreatic glucagon comprising administering the above described antigen to a mammal and recovering the antibody produced.
Inventor(s): Nishino; Tomoyoshi (Tokushima, JP)
Assignee: Otsuka Pharmaceutical Co., Ltd. (Tokyo, JP)
Application Number:06/077,221
Patent Claims:1. A peptide-protein complex antigen consisting essentially of a peptide of the formula (I) ##STR5## wherein m is 0 or 1, as a hapten, linked by a dialdehyde of the formula (II)

wherein n is an integer of 1 to 5, to a protein carrier selected from the group consisting of equine serum albumin, bovine serum albumin, rabbit serum albumin, human serum albumin, equine serum globulin, bovine serum globulin, rabbit serum globulin and human serum globulin conventionally used to prepare antigen.

2. The antigen of claim 1, wherein m is 0.

3. The antigen of claim 1, wherein m is 1.

4. The antigen of claim 1, wherein there is present peptide of the formula (I), wherein m is 0 and also peptide of the formula (I) wherein m is 1.

5. The antigen of claim 1, wherein said dialdehyde is malonaldehyde, succinaldehyde, glutaraldehyde, or adipaldehyde.

6. The antigen of claim 1, wherein there is on the average 5 to 15 moles of peptide per mole of carrier protein.

7. The antigen of claim 6, containing on the average 9 to 12 moles of peptide per mole of carrier protein.

8. The antigen of claim 1, wherein the proportion by weight of said protein as a carrier to said peptide represented by the general formula (I) ranges from about 2:1 to about 6:1 and wherein the molar proportion of said dialdehyde represented by the general formula (II) to said peptide represented by the general formula (I) ranges from about 5:1 to about 10:1.

9. The antigen of claim 1, wherein the carrier is bovine serum albumin.

10. The antigen of claim 1, wherein n is 3.

11. The antigen of claim 10, wherein the carrier is bovine serum albumin.

12. The antigen of claim 10, wherein m is 0.

13. The antigen of claim 10, wherein m is 1.

Details for Patent 4,272,433

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Grifols Therapeutics Llc ALBUKED, PLASBUMIN-20, PLASBUMIN-25, PLASBUMIN-5 albumin (human) For Injection 101138 10/21/1942 ⤷  Try a Trial 1997-02-10
Baxalta Us Inc. BUMINATE, FLEXBUMIN albumin (human) Injection 101452 03/03/1954 ⤷  Try a Trial 1997-02-10
Csl Behring Ag ALBURX albumin (human) Injection 102366 07/23/1976 ⤷  Try a Trial 1997-02-10
Grifols Biologicals Llc ALBUTEIN albumin (human) Injection 102478 08/15/1978 ⤷  Try a Trial 1997-02-10
Instituto Grifols, S.a. HUMAN ALBUMIN GRIFOLS albumin (human) Injection 103352 02/17/1995 ⤷  Try a Trial 1997-02-10
Instituto Grifols, S.a. HUMAN ALBUMIN GRIFOLS albumin (human) Injection 103352 06/11/2003 ⤷  Try a Trial 1997-02-10
Csl Behring Llc ALBUMINAR, ALBUMINAR-20, ALBUMINAR-25, ALBUMINAR-5 albumin (human) Injection 103955 03/17/2000 ⤷  Try a Trial 1997-02-10
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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