Claims for Patent: 10,196,611

Summary for Patent: 10,196,611

Title:	Device and surgical system for rapid aseptic isolation and concentration of autologous fat derived stem cells
Abstract:	The present disclosure provides an apparatus for harvesting stem cells from fat tissue. The apparatus may include a first transducer coupled to a first end of an resonant horn to form an ultrasonic resonator. The resonant horn may include an elongated body having a plurality of through-holes configured to accommodate a plurality of specimen containers that are positioned substantially perpendicular to the elongated body. The apparatus may also include a wave generator coupled to the first transducer to generate an ultrasonic wave, wherein the elongated body has a length that is multiples of the half-wavelength of the ultrasonic wave.
Inventor(s):	Henley; Julian (Fort Collins, CO)
Assignee:
Application Number:	15/016,561
Patent Claims:	1. An apparatus for harvesting stem cells from fat tissue, the apparatus comprising: a first transducer coupled to a first end of a resonant horn of an ultrasonic resonator, the resonant horn including an elongated body having a plurality of through-holes configured to accommodate a plurality of specimen containers that are positioned substantially perpendicular to the elongated body; and a wave generator coupled to the first transducer to generate an ultrasonic wave, wherein the elongated body has a length that is multiples of the half-wavelength of the ultrasonic wave, wherein the plurality of through-holes in the resonant horn is positioned at the maximum energy transfer locations of the resonator. 2. The apparatus of claim 1, further comprising a second transducer coupled to an opposing end of the resonant horn. 3. The apparatus of claim 2, wherein the wave generator is configured to generate first and second square waves that have a 180 degrees phase shift for the respective first and second transducers. 4. The apparatus of claim 1, wherein the first transducer comprises a piezoelectric transducer. 5. The apparatus of claim 1, wherein the resonant horn comprises one of titanium or aluminum. 6. The apparatus of claim 1, wherein the plurality of specimen containers comprises two, four, or higher even numbers. 7. The apparatus of claim 1, wherein the specimen container comprises a cap that is configured to extract stem cells by using a syringe. 8. The apparatus of claim 1, wherein the specimen container comprises a plurality of ridges inside the specimen container configured to provide acoustic coupling between the horn and the fat tissue to maximize the energy output to the fat tissue. 9. The apparatus of claim 1, wherein the specimen container comprises a plurality of grooves outside the specimen container configured to provide acoustic coupling between the horn and the fat tissue to maximize the energy output to the fat tissue. 10. The apparatus of claim 1, further comprising a temperature sensor coupled to the resonant horn configured to measure specimen temperature in the specimen container during ultrasonic disruption. 11. The apparatus of claim 10, further comprising a safety circuit for adjusting the duty cycle of the wave generator in real time based upon the feedback from the temperature sensor. 12. The apparatus of claim 1, further comprising an acceleration sensor coupled to the horn and a self-tuning circuit configured to boost optimal resonance based upon the feedback from the acceleration sensor. 13. The apparatus of claim 1, wherein each of the plurality of specimen containers is configured to be sealed to preserve the sterility of the fat tissue during harvesting the stem cells. 14. The apparatus of claim 1, further comprising a self-tuning circuit configured to tune the ultrasonic energy when the plurality of specimen containers containing the fat tissue is in the plurality of through-holes of the resonant horn. 15. A method for sterilely extracting stem cells from fat, the method comprising separating stem cells from the fat by using the apparatus of claim 1 in a medical care room to sterilely extract stem cells from the fat, wherein the plurality of through-holes in the resonant horn is positioned at the maximum energy transfer locations of the resonator. 16. The method of claim 15, wherein the method takes less than 30 minutes. 17. The method of claim 15, the step of separating stem cell comprising placing the fat tissue without reagent in each of the plurality of specimen containers and applying electro-mechanical energies from the ultrasonic resonator to the fat tissue. 18. The method of claim 15, the step of separating stem cell further comprising placing the fat tissue mixed with reagent in each of the plurality of specimen containers to accelerate the enzymatic breakdown of the fat tissue and applying electro-mechanical energies from the ultrasonic resonator to the fat tissue mixed with reagent. 19. The method of claim 18, wherein the reagent comprises one of collagenase, enzymatic digestion, or enzymes. 20. The apparatus of claim 2, wherein the ultrasonic resonator further comprises the plurality of specimen containers containing the fat tissue. 21. The apparatus of claim 20, wherein the apparatus creates sufficient energy to achieve desired fat tissue disruption without compromising sterility of the fat tissue.

Details for Patent 10,196,611

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2035-02-07