Claims for Patent: 10,173,938

Summary for Patent: 10,173,938

Title:	Process for producing a biofertilizer comprising the steps of solid-state fermentation, immobilization through allophane nanoparticles and a second fermentation; and the said biofertilizer
Abstract:	Process for producing a biofertilizer, comprising the following steps: a) solid-state fermentation to produce enzymes and nutrients critical for plant nutrition; b) immobilization through allophane nanoparticles of the enzymes and substrates produced during stage a); and c) a second fermentation to favor the development of microorganisms that improve the quality of the biofertilizer. Protection is also sought for the biofertilizer that is produced from this process.
Inventor(s):	Rosas Gajardo; Anali Estela (Concepcion, CL), Lopez Emparan; Roxana Sofia (Concepcion, CL)
Assignee:	UNIVERSIDAD DE CONCEPCION (Concepcion, CL) UNIVERSIDAD DE TALCA (Concepcion, CL)
Application Number:	15/109,204
Patent Claims:	1. Process for producing a biofertilizer comprising the following stages: a) subjecting an agricultural, livestock and agro-industrial waste to a first solid-state fermentation (SSF) for producing organic substrates, microorganisms and enzymes selected from the group consisting of phosphatases, sulfatases, asparaginases and glutaminases; b) immobilizing the enzymes and substrates produced during stage a) by adding allophane nanoparticles, wherein the allophane nanoparticles are spherules having a diameter of 3-8 nm that form porous aggregates, to obtain an intermediate product, and protecting the enzymes and organic substrates from microbial degradation by the microorganisms; and c) subjecting the intermediate product obtained from stage b) to a second solid-state fermentation (SFF) to reduce the number of pathogenic microorganisms thereby improving the quality of the obtained biofertilizer which is innocuous to plants. 2. Process for producing a biofertilizer according to claim 1, wherein stage a) of the process is carried out in reactors or piles. 3. Process for producing a biofertilizer according to claim 1, wherein stage a) is carried out at a temperature of between 25.degree. C. and 70.degree. C., with a moisture level of between 55% and 80%, and lasts between 12 and 18 days when carried out in reactors and between 5 and 10 weeks when carried out in piles. 4. Process for producing a biofertilizer according to claim 1, wherein in stage b) allophane is added in a proportion of between 10% and 40% weight/weight with regard to a dry fermented matter. 5. Process for producing a biofertilizer according to claim 1, wherein the SSF stage c) lasts between 30 and 60 days and is carried out at a temperature of between 15.degree. C. and 50.degree. C., with a moisture level of between 60% and 85%. 6. The process for producing a biofertilizer according to claim 1, wherein the obtained biofertilizer has a concentration of encapsulated enzymes in the allophane comprising 1.09-4.4 IU/g of alkaline phosphatase, 0.2-1.2 IS/g of acid phosphatase, 0.05-0.1 IU/g of arylsulfatase, 0.6-1.05 IU/g of L-asparaginase, and 3.0-4.6 IU/g of L-glutaminase.

Details for Patent 10,173,938

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2033-12-30