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Last Updated: March 29, 2024

Claims for Patent: 10,168,318


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Summary for Patent: 10,168,318
Title:Method of measuring cell-substrate impedance in living cells to identify compounds affecting receptor tyrosine kinase (RTK) activity and pathways
Abstract: A method of identifying a potential therapeutic compound that affects a Receptor Tyrosine Kinase (RTK) pathway in cancer cells, which includes: providing a device capable of measuring cell-substrate impedance; culturing cancer cells in serum-free media in at least two wells of the device; adding to a first well a proposed therapeutic compound that affects a RTK pathway and a RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells and optionally determining cell indices from the monitored cell-substrate impedance; and determining a difference in impedance or optionally cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells.
Inventor(s): Abassi; Yama A. (San Diego, CA), Yu; Naichen (San Diego, CA), Atienza; Josephine (San Diego, CA), Xu; Xiao (San Diego, CA), Wang; Xiaobo (San Diego, CA)
Assignee: ACEA Biosciences, Inc. (San Diego, CA)
Application Number:15/613,052
Patent Claims:1. A method of identifying a potential therapeutic compound that affects a Receptor Tyrosine Kinase (RTK) pathway in cancer cells, the method comprising: a) providing a device capable of measuring cell-substrate impedance, wherein the device comprises at least two wells, each well comprising an electrode array, further wherein the device is operably connected to an impedance analyzer; b) culturing cells embodied as cancer cells in serum-free media in the at least two wells; c) adding to a first well a proposed therapeutic compound that affects an RTK pathway and an RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; d) continuously monitoring cell-substrate impedance of the at least two wells and optionally determining cell indices from the monitored cell-substrate impedance; and e) determining a difference in impedance or optionally in cell index between the test well and control well; and if significantly different, f) concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells.

2. The method of claim 1, wherein the cancer cells are primary cells.

3. The method according to claim 1, wherein the cancer cells are selected from the group consisting of breast cancer cells, lung cancer cells, and colon cancer cells.

4. The method according to claim 1, wherein the cancer cells are selected from the group consisting of ovarian cancer cells, prostate cancer cells, hepatosarcoma cells, epidermoid cancer cells, and fibrosarcoma cells.

5. The method of claim 1, wherein the proposed therapeutic compound is a kinase inhibitor.

6. The method of claim 1, wherein the proposed therapeutic compound is Trastuzumab (HERCEPTIN) or 4- Quinazolinamine N-(3-chloro-4-fluorophenyl)-7-methoxy-6-[3-(4-morpholinyl) propoxy](GEFITINIB).

7. The method of claim 1, wherein the proposed therapeutic compound is added before the RTK stimulating factor.

8. The method of claim 1, wherein the RTK stimulating factor is a growth factor.

9. The method of claim 1, wherein the RTK stimulating factor is selected from the group consisting of epidermal growth factor (EGF), platelet derived growth factor (PDGF), a nerve growth factor (NGF), hepatocyte growth factor (HGF), fibroblast growth factor (FGF), and an antibody capable of activating RTK.

Details for Patent 10,168,318

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Genentech, Inc. HERCEPTIN trastuzumab For Injection 103792 09/25/1998 ⤷  Try a Trial 2022-12-20
Genentech, Inc. HERCEPTIN trastuzumab For Injection 103792 02/10/2017 ⤷  Try a Trial 2022-12-20
Genentech, Inc. HERCEPTIN HYLECTA trastuzumab and hyaluronidase-oysk Injection 761106 02/28/2019 ⤷  Try a Trial 2022-12-20
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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