Claims for Patent: 10,047,353
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Summary for Patent: 10,047,353
Title: | Grimonitia-hollisae-derived recombinant collagenase and enzyme agent for cell and tissue dissociation |
Abstract: | Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae-derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae-derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity. |
Inventor(s): | Teramura; Naoko (Tokyo, JP), Iijima; Katsumasa (Tokyo, JP), Hayashida; Osamu (Tokyo, JP), Tanaka; Keisuke (Tokyo, JP), Hattori; Shunji (Tokyo, JP), Okitsu; Teru (Tokyo, JP), Takeuchi; Shoji (Tokyo, JP) |
Assignee: | Nippi, Incorporated (Tokyo, JP) The University of Tokyo (Tokyo, JP) |
Application Number: | 15/123,986 |
Patent Claims: | 1. A recombinant collagenase that is genetically derived from Grimontia hollisae collagenase (SEQ ID No: 1) which comprises, from N terminal to C-terminal, a
collagenase catalytic domain, a linker region sequence, and a prepeptidase C-terminal domain, which recombinant collagenase comprises at least the collagenase catalytic domain but does not comprise at least the prepeptidase C-terminal domain of Grimontia
hollisae collagenase; wherein the recombinant collagenase has the amino acid sequence represented in SEQ ID No: 3, SEQ ID No: 4, SEQ ID No: 14, or SEQ ID No:15.
2. An enzyme agent for cell and tissue dissociation comprising the recombinant collagenase according to claim 1. 3. The enzyme agent for cell and tissue dissociation according to claim 2 that is used for separation of one or more cells selected from the group consisting of: pancreatic islets, a liver, a heart, lungs, kidneys, a spleen, adrenal glands, muscles, thyroid glands, salivary glands, parotid gland acini, mammary tissues, bones, cartilages, endothelial cells, epithelial cells, adipose tissues and fibroblasts. 4. An enzyme agent for digestion of collagen IV, collagen V, or collagen VI comprising the recombinant collagenase according to claim 1. |
Details for Patent 10,047,353
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Smith & Nephew, Inc. | SANTYL | collagenase | Ointment | 101995 | 06/04/1965 | ⤷ Try a Trial | 2034-03-06 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
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